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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: long non-coding RNA,Polymorphism,Acute Lymphoblastic Leukemia,lnc-LAMC2-1:1,CASC8
- چکیده:
- چکیده انگلیسی:
Long non-coding RNAs (lncRNAs) are a novel class of non-protein coding RNAs that are involved in a wide variety of biological processes. There are limited data regarding the impact of lnc-LAMC2-1:1 rs2147578 as well as CASC8 rs10505477 T>C polymorphisms on cancer development. Here we examined for the first time whether rs2147578 and rs10505477 polymorphisms are associated with childhood acute lymphoblastic leukemia (ALL) in a total of 110 cases and 120 healthy controls. Genotyping was achieved by the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. The rs2147578 variant increased the risk of ALL in codominant (OR=4.33, 95%CI=2.00-9.37, p<0.0001, CG vs CC, and OR=5.81, 95%CI=2.30-14.69, p=0.0002, GG vs CC), dominant (OR=4.63, 95%CI=2.18-9.86, p<0.0001, CG+GG vs CC), overdominant (OR=1.74, 95%CI=1.02-2.97, p=0.0444, CG vs CC+GG) and allele (OR=1.91, 95%CI=1.32-2.77, p=0.0008, G vs C) inheritance models tested. No significant association was found between the CASC8 rs10505477 T>C variant and risk of childhood ALL. In conclusion, the present study revealed that the lnc-LAMC2-1:1 rs2147578 polymorphism may be a risk factor for developing childhood ALL. Further studies with larger sample sizes with different ethnicities are now required to confirm our findings.- انتشار مقاله: 17-07-1395
- نویسندگان: Mohammad Hashemi,Gholamreza Bahari,Majid Naderi,Simin Sadeghi-Bojd,Mohsen Taheri
- مشاهده
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: Telomere Length,TERT genetic polymorphisms, Childhood acute lymphoblastic leukemia
- چکیده:
- چکیده انگلیسی: Background: Telomeres are involved in chromosomal stability, cellular immortality and tumorigenesis. Human
telomerase reverse transcriptase (TERT) is essential for the maintenance of telomere DNA length. Recently, a variable
tandem-repeats polymorphism, MNS16A, located in the downstream region of the TERT gene, was reported to have
an effect on TERT expression and telomerase activity. Previous studies have linked both relative telomere length
(RTL) and TERT variants with cancer. Therefore, we evaluated associations between RTL, TERT gene polymorphisms
(hTERT, rs2735940 C/T and MNS16A Ins/Del) and risk of childhood acute lymphoblastic leukemia (ALL) in an Iranian
population. Methods: RTL was determined by a multiplex quantitative PCR-based method, and variants of the hTERT,
rs2735940 C/T and MNS16A Ins/Del, were genotyped by amplification refractory mutation system PCR (ARMS-PCR),
and PCR, respectively. Results: Our results indicated that RTL was shorter in ALL patients (1.53±0.12) compared to
the control group (2.04±0.19) (P=0.029). However, no associations between hTERT gene variants or haplotypes and
the risk of childhood ALL were observed (P>0.05). Also hTERT polymorphisms were not associated with RTL or
patient clinicopathological characteristics, including age (P=0.304), sex (P=0.061) organomegally (P=0.212) CSF
involvement (P=0.966) or response to treatment (P=0.58). Conclusions: We found that telomere attrition may be
related to the pathogenesis of childhood ALL, irrespective to TERT variants.- انتشار مقاله: 04-05-1396
- نویسندگان: Ebrahim Eskandari,Mohammad Hashemi,Majid Naderi,Gholamreza Bahari,Vahid Safdari,Mohsen Taheri
- مشاهده