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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: Iranian Journal of Pathology
- نوع مقاله: Journal Article
- کلمات کلیدی: Hirschsprung Disease,immunohistochemistry,Calretinin,ganglion cell,intrinsic nerve fibers
- چکیده:
- چکیده انگلیسی: Background: Hirschsprung disease is a complex genetic disorder of the enteric nervous system (ENS), often called congenital aganglionic megacolon and characterized by the absence of enteric neurons along a variable length of the intestine. The definitive diagnosis of Hirschsprung disease relies on histologic and/or histochemical staining of sections from suction rectal biopsies. Calretinin immunohistochemistry (IHC) may be a useful in its diagnosis. This study aimed to proof the usefulness of immunohistochemical staining for calretinin in rule out of Hirschsprung disease.
Methods: Paraffin blocks and slides were retrieved from the pathology archives of Ali Asghar Hospital, Tehran, Iran from 2007 to 2011 with pathology report based on the presence (14 patients) or absence (70 patients) of ganglion cells and transitional zone anatomical region (10 patients). Slides were stained with hematoxylin and eosin method to confirm the initial diagnosis was verification again. After preparing the slides, they were stained by IHC for calretinin. Then, the results were analyzed using SPSS software.
Results: In most patients, IHC for calretinin provided highly compatible results with hematoxylin-eosin findings in diagnosis of Hirschsprung disease. The values of specificity and accuracy between calretinin and standard histology (H&E) compared by the Fisher exact test declared calretinin presented significantly higher specificity and accuracy values than H&E staining (P <0.0001).
Conclusion: Calretinin IHC overcomes most of the difficulties encountered using the histology hematoxylin-eosin. Then, IHC for calretinin is a good ancillary method used by pathologists in diagnosis of Hirschsprung disease.- انتشار مقاله: 04-08-1394
- نویسندگان: Nasser Rakhshani,Mohammadreza Araste,Farid Imanzade,Mahshid Panahi,Fahimeh Safarnezhad Tameshkel,Masoud Reza Sohrabi,Mohammad Hadi Karbalaie Niya,Farhad Zamani
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Pathology
- نوع مقاله: Journal Article
- کلمات کلیدی: Chronic HCV infection,Sustained virologic response,Interleukin 28B polymorphism
- چکیده:
- چکیده انگلیسی: Background: Nowadays, the immune response to hepatitis C (HCV) treatment has become a crucial issue mostly due to the interleukin 28B (IL-28B) polymorphism effects in chronic HCV patients. The aim of this study was to detect the polymorphism of IL-28B gene (rs12979860) in HCV genotype 1 patients treated with pegylated Interferon and Ribavirin. Methods: From the 2010 to 2012, a total of 115 peripheral blood mononuclear cells (PBMCs) of HCV patients who presented to Gastrointestinal & Liver Disease Research Center (GILDRC), Firoozgar Hospital, Tehran, Iran were enrolled in this retrospective cross sectional study. Samples were then categorized based on the presence of sustained virologic response (SVR and no-SVR). Variables including age, gender, serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels of the two groups were investigated based on different IL-28B genotypes. Results: Analysis by the variables of age and gender showed a mean age ± SD of 42.1±14.0 and gender variability of 44 females (38.2%) and 71 males (61.8%). Adding up these results, the analysis of ALT levels revealed that there was between 293 and 14 mg/ml; AST levels ranged between 217 and 17 mg/ml; the viral load (HCV RNA) ranged between 7,822,000 and 50 IU/ml; the prevalence of CC, CT and TT genotypes were 90.9%, 54% and 25.0%. Conclusion: IL-28B polymorphism has an effective impact on the therapeutic response to ribavirin and peginterferon combination therapy in chronic HCV patients infected by different genotypes. This polymorphism is crucial in natural clearance. How to cite this article: Safarnezhad Tameshkel F, Karbalaie Niya MH, Sohrabi M, Panahi M, Zamani F, Imanzade F, et al. Polymorphism of IL-28B Gene (rs12979860) in HCV Genotype 1 Patients Treated by Pegylated Interferon and Ribavirin. Iran J Pathol. 2016; 11(3):216-21.
- انتشار مقاله: 03-05-1394
- نویسندگان: Fahimeh Safarnezhad Tameshkel,Mohmmad Hadi Karbalaie Niya,Masuodreza Sohrabi,Mahshid Panahi,Farhad Zamani,Farid Imanzade,Nasser Rakhshani
- مشاهده
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: Phylogenetic analysis,Colorectal cancer (CRC),Human bocavirus (HBoV)
- چکیده:
- چکیده انگلیسی: Background: Colorectal cancer (CRC) as a worldwide human health concern is identified being a multifactorial
subject that infection with specific viral particles such as oncogenic viruses is research interest. Human bocavirus (HBoV)
as a recent isolated virus has been investigated in many respiratory and enteric diseases but rare studies evaluates it in
tissue specimens especially in cancerous sections. The aim of this study was to detect the presence of HBoV genome
and its genotyping in CRC patient’s tissue and compare the result with matched healthy control group tissue. Method:
in this retrospective case-control study, CRC cases were sporadic and non-familial cancerous while control subjects had
healthy or non-malignant lesions in colon tissue. A conventional-PCR performed by specific primers for HBoV VP1
gene. After sequencing of positive PCR products, raw data used for trimming and alignment by bioinformatics software
CLC Main Workbench 5 and MEGA5. SPSS v.22 used for statistical calculations. Result: a total of 157 subjects were
participated that 66 were diagnosed as CRC cases and 91 were non-CRC colon tissue as control group that matched by
the cases. The mean age (y) ± standard deviation of each case and control groups were 59.35±14.48 and 57.21±14.66,
respectively. PCR results showed there were 1.3% (2/157) HBoV positive (of each groups one was positive). Sequencing
analysis showed all were HBoV-1 genotype. Conclusion: our study showed there are low rate of HBoV genome in
Iranian CRC and non-CRC colon tissue. Furthermore, the predominant genotype in our studied subsets were HBoV-1
according to phylogenetic analysis.- انتشار مقاله: 01-05-1397
- نویسندگان: Mohammad Hadi Karbalaie Niya,Hossein Ajdarkosh,Fahimeh Safarnezhad Tameshkel,Mahshid Panahi,Maryam Tabasi,Behnaz Bouzari,Mahdi Alemrajabi,Hossein Keyvani
- مشاهده
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: Human papillomavirus (HPV),Head and Neck Squamous Cell Carcinoma (HNSCC),Molecular epidemiology,Nested-PCR,INNO-LiPA HPV genotyping
- چکیده:
- چکیده انگلیسی:
Background: Head and neck squamous cell carcinomas (HNSCC) are a major health issue in many parts of the world. Recently, attention has focused on the human papilloma virus (HPV) as a potential causative agent for HNSCC. This study aimed to survey HPV occurrence in HNSCCs as part of a comprehensive molecular epidemiology approach. Methods: In this retrospective study, patients were recruited from hospitals affiliated to the Iran University of Medical Sciences, Tehran, Iran. Formalin-fixed paraffin-embedded (FFPE) blocks were subjected to DNA isolation by QIAamp® DNA FFPE Tissue Kit and nested PCR, HPV-16 specific conventional PCR, and extra INNO-LiPA HPV genotyping assays were subsequently performed. PCR products were purified with a High Pure PCR Product Purification Kit and sequenced with an ABI 3730 XL sequencer. CLC Main Workbench 5 and MEGA5 bioinformatics software was used to analyze the raw data and to create the phylogenetic tree. SPSS v.20 was applied for statistical analysis. Results: A total of 156 FFPE blocks were collected from 2011 to 2017. Total mean age (y) of participants was 60.5 ± 12.6; 77.6 % (121/156) being men and 22.4% (35/156) e women. Overall, 5/156 (3.2%) patients (3 females and 2 males) were found to be HPV positive using the three methods. HPV genotyping revealed HPV types 16, 2, 27, and 43 in these malignancies. Tumor location and lymph node involvement indicated significant differences between the sexes. Conclusion: Although high risk HPV genotypes have been associated with HNSCCs, our findings indicate a potential of low risk HPV types to also contribute to such malignancies.- انتشار مقاله: 01-05-1396
- نویسندگان: Mohammad Hadi Karbalaie Niya,Fahimeh Safarnezhad Tameshkel,Mahshid Panahi,Farah Bokharaei Salim,Seyed Hamid Reza Monavari,Hossein Keyvani
- مشاهده
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: Human papillomavirus (HPV),Head and Neck Squamous Cell Carcinoma (HNSCC),Molecular epidemiology,Nested-PCR,INNO-LiPA HPV genotyping
- چکیده:
- چکیده انگلیسی:
Background: Head and neck squamous cell carcinomas (HNSCC) are a major health issue in many parts of the world. Recently, attention has focused on the human papilloma virus (HPV) as a potential causative agent for HNSCC. This study aimed to survey HPV occurrence in HNSCCs as part of a comprehensive molecular epidemiology approach. Methods: In this retrospective study, patients were recruited from hospitals affiliated to the Iran University of Medical Sciences, Tehran, Iran. Formalin-fixed paraffin-embedded (FFPE) blocks were subjected to DNA isolation by QIAamp® DNA FFPE Tissue Kit and nested PCR, HPV-16 specific conventional PCR, and extra INNO-LiPA HPV genotyping assays were subsequently performed. PCR products were purified with a High Pure PCR Product Purification Kit and sequenced with an ABI 3730 XL sequencer. CLC Main Workbench 5 and MEGA5 bioinformatics software was used to analyze the raw data and to create the phylogenetic tree. SPSS v.20 was applied for statistical analysis. Results: A total of 156 FFPE blocks were collected from 2011 to 2017. Total mean age (y) of participants was 60.5 ± 12.6; 77.6 % (121/156) being men and 22.4% (35/156) e women. Overall, 5/156 (3.2%) patients (3 females and 2 males) were found to be HPV positive using the three methods. HPV genotyping revealed HPV types 16, 2, 27, and 43 in these malignancies. Tumor location and lymph node involvement indicated significant differences between the sexes. Conclusion: Although high risk HPV genotypes have been associated with HNSCCs, our findings indicate a potential of low risk HPV types to also contribute to such malignancies.- انتشار مقاله: 01-05-1396
- نویسندگان: Mohammad Hadi Karbalaie Niya,Fahimeh Safarnezhad Tameshkel,Mahshid Panahi,Farah Bokharaei Salim,Seyed Hamid Reza Monavari,Hossein Keyvani
- مشاهده
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: Human papillomavirus (HPV),Head and Neck Squamous Cell Carcinoma (HNSCC),Molecular epidemiology,Nested-PCR,INNO-LiPA HPV genotyping
- چکیده:
- چکیده انگلیسی:
Background: Head and neck squamous cell carcinomas (HNSCC) are a major health issue in many parts of the world. Recently, attention has focused on the human papilloma virus (HPV) as a potential causative agent for HNSCC. This study aimed to survey HPV occurrence in HNSCCs as part of a comprehensive molecular epidemiology approach. Methods: In this retrospective study, patients were recruited from hospitals affiliated to the Iran University of Medical Sciences, Tehran, Iran. Formalin-fixed paraffin-embedded (FFPE) blocks were subjected to DNA isolation by QIAamp® DNA FFPE Tissue Kit and nested PCR, HPV-16 specific conventional PCR, and extra INNO-LiPA HPV genotyping assays were subsequently performed. PCR products were purified with a High Pure PCR Product Purification Kit and sequenced with an ABI 3730 XL sequencer. CLC Main Workbench 5 and MEGA5 bioinformatics software was used to analyze the raw data and to create the phylogenetic tree. SPSS v.20 was applied for statistical analysis. Results: A total of 156 FFPE blocks were collected from 2011 to 2017. Total mean age (y) of participants was 60.5 ± 12.6; 77.6 % (121/156) being men and 22.4% (35/156) e women. Overall, 5/156 (3.2%) patients (3 females and 2 males) were found to be HPV positive using the three methods. HPV genotyping revealed HPV types 16, 2, 27, and 43 in these malignancies. Tumor location and lymph node involvement indicated significant differences between the sexes. Conclusion: Although high risk HPV genotypes have been associated with HNSCCs, our findings indicate a potential of low risk HPV types to also contribute to such malignancies.- انتشار مقاله: 01-05-1396
- نویسندگان: Mohammad Hadi Karbalaie Niya,Fahimeh Safarnezhad Tameshkel,Mahshid Panahi,Farah Bokharaei Salim,Seyed Hamid Reza Monavari,Hossein Keyvani
- مشاهده