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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: Nanomedicine Research Journal
- نوع مقاله: Journal Article
- کلمات کلیدی: Morphine,naloxone,silver nanoparticles,c-Fos,Central amygdala
- چکیده:
- چکیده انگلیسی: Abstract
Background: Silver nanoparticles (Ag-NPs) that are used daily in care service can enter the body and create free radicals. Despite the toxicity at high concentrations, these particles are non-toxic and useful at low concentrations. Thus, we investigated the effectiveness of nontoxic Ag-NPs to interfere with the aversive effect of naloxone (NLX) and low expression of c-Fos during testing of morphine-induced conditioned place preference (CPP) in rats. Methods: The Wistar rats (weighing 300-350 g) were cannulated bilaterally by stereotaxic apparatus for the CeA (AP= –2.12 mm; L= ±4.1 mm; V= 7.8 mm). CPP was conducted via a three-phase unbiased procedure. Morphine (0.5-7.5 mg/kg) was injected subcutaneously (sc) during the conditioning phase. NLX (0.4 µg/rat) was given, intra-CeA, 10 min before the test. Ag-NPs (0.01 µg/rat) was administered prior to the antagonist. The control group received saline (1 µL/rat, intra-CeA). c-Fos expression was quantified immunohistochemically in rats subsequent the injections: Results: The CeA and hippocampal cornu ammonis 1 (CA1) of rats that treated by NLX showed low c-Fos protein levels during testing, whereas levels of protein were high in the brains of morphine conditioned rats. Interestingly, both areas (CeA and CA1) showed similar increases in protein levels when the injection of NLX was combined with the Ag-NPs. However, these regions were not significantly different in the single Ag-NPs receiving and control groups. Conclusions: This indicates that the two regions interact with each other when NLX is injected and that in presence of Ag-NPs the protein levels are elevated in the regions.- انتشار مقاله: 13-05-1399
- نویسندگان: Mahnaz Rahimpour,Manizheh Karami,Ali Haeri Rohani
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Basic Medical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: Amygdala,morphine dependence,naloxone,Nitric oxide
- چکیده:
- چکیده انگلیسی: Objective(s) Single injection of naloxone, a selective antagonist of morphine, prior to the drug conditioning testing was used to investigate on morphine dependence. Materials and Methods Conditioning to morphine (2.5-10 mg/kg, s.c.) was established in adult male Wistar rats (weighing 200-250 g) using an unbiased procedure. Nitric oxide agents were microinjected into the central amygdala prior to naloxone-paired place conditioning testing. Results The results showed that morphine produced a significant dose-dependent place preference in animals. Naloxone (0.1-0.4 mg/kg, i.p.) injections pre-testing of the response to morphine (7.5 mg/kg, s.c.) caused a significant aversion at the higher doses (0.4 mg/kg, i.p.). This response was reversed by microinjection of L-arginine (0.3-3 μg/rat, intra-central amygdala) prior to naloxone on the day of the testing. The response to L-arginine was blocked by pre-injection of NG-nitro-L-arginine methyl ester (L-NAME) (intra-central amygdala). Conclusion A single injection of naloxone on the test day of morphine place conditioning may simply reveal the occurrence of morphine dependence in rats, and that the nitric oxide in the central amygdala most likely plays a key role in this phenomenon.
- انتشار مقاله: 30-06-1394
- نویسندگان: Mahnaz Rahimpour,Manizheh Karami,Sara Karimi,Abbas Haghparast,Mohammad Reza Jalali,Farzaneh Sabouni
- مشاهده