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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: Asian Journal of Green Chemistry
- نوع مقاله: Journal Article
- کلمات کلیدی: Spirulina platensis,Alkalase,ACE inhibitory peptide
- چکیده:
- چکیده انگلیسی: Spirulina has attracted a great attention as it contains many nutrients, such as protein, vitamins and minerals. Spirulina contains peptides that have therapeutic and beneficial effects on the human body. Some medicinal properties of the biological peptides of Spirulina platensis include antioxidants, antifungal, antimicrobial, anti-diabetes and anticoagulant activity. This study provides an overview of the biological peptides derived from Spirulina and some biological activities with health benefits. In this study peptide Ile-Gln-Pro from spirulina platalsis was isolated using an alkalase enzyme and investigate its inhibitory effect on the angiotensin I-converting enzyme (ACE). This peptide was purified by gel filtration chromatography and Reverse-phase high-performance liquid chromatography (RP-HPLC) Enzymatic kinetic studies showed a non-competitive inhibitory activity of this peptide, and the Ki value was 5.8 μm.
- انتشار مقاله: 03-02-1397
- نویسندگان: Gholamreza Mahdieh,Mohamad Fazilati,Mahdieh Izadi
- مشاهده
- جایگاه : پژوهشی
- مجله: Asian Journal of Green Chemistry
- نوع مقاله: Journal Article
- کلمات کلیدی: Phycocyanin,Spirulina platensis,NADPH Oxidase
- چکیده:
- چکیده انگلیسی: Phycocyanin is a blue pigment and water-soluble biliprotein from the spirulina platensis. It has great medical and medicinal properties and has been used as a nutritional supplements. Phycocyanin is also a natural and powerful antioxidant and anti-inflammatory. In this study, the phycocyanin of Spirulina platensis was extracted by using two enzymatic and ultrasonic methods and purified by ammonium sulfate precipitation and dialysis. The UV spectrophotometer absorption of the extracted sample showed a broad peak range at 280, 615, and 652 nm. By using The FT-IR results confirmed the structure and molecular bonds from extracted and purificated phycocyanin. The concentrations obtained at the enzymatically was 0.405 mg/mL and for sonication 0.422 mg/mL. By using the SDS PAGE method, bands of phycocyanin were identified and the molecular weight was determined between 19-14 KD. The antioxidant and anti-inflammatory activity of the phycocyanin was approved with the reaction by DPPH and HOCl.
- انتشار مقاله: 09-01-1397
- نویسندگان: Mahdieh Izadi,Mohammad Fazilati
- مشاهده