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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: Breast cancer,Polymorphism,Vitamin D,Vitamin D receptor,Fok1
- چکیده:
- چکیده انگلیسی: Breast cancer is the leading cause of death among women worldwide. It is a multi-factorial disease caused by
genetic and environmental factors. Vitamin D has been hypothesized to lower the risk of breast cancer via the nuclear
vitamin D receptor (VDR). Genetic variants of these vitamin D metabolizing genes may alter the bioavailability of
vitamin D, and hence modulate the risk of breast cancer. Materials and Methods: The distribution of Fok1 VDR gene
(rs2228570) polymorphism and its association with breast cancer was analysed in a case–control study based on 125
breast cancer patients and 125 healthy females from North Indian population, using PCR-RFLP. An In silico exploration
of the probable mechanism of increased risk of breast cancer was performed to investigate the role of single nucleotide
polymorphisms (SNPs) in cancer susceptibility. Results: The Fok1 ff genotype was significantly associated with an
increased risk of breast cancer (p=0.001; χ2=13.09; OR=16.909; %95 CI=2.20 - 130.11). In silico analysis indicated
that SNPs may lead to a loss in affinity of VDR to calcitriol, and may also cause the impairment of normal interaction
of liganded VDR with its heterodimeric partner, the retinoid X receptor (RXR), at protein level, thereby affecting target
gene transcription. Conclusion: Breast cancer risk and pathogenesis in females can be influenced by SNPs. SNPs in
VDR may cause alterations in the major molecular actions of VDR, namely ligand binding, heterodimerization and
transactivation. VDRE binding and co-activator recruitment by VDR appear to be functionally inseparable events that
affect vitamin D-elicited gene transcription. This indicates that breast cancer risk and pathogenesis in females may be
influenced by SNPs.- انتشار مقاله: 04-05-1397
- نویسندگان: Sana Raza,Anupam Dhasmana,Madan Lal Brahma Bhatt,Mohtashim Lohani,Jamal M Arif
- مشاهده
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: Gene expression,immunohistochemistry,OSCC,c-Fos
- چکیده:
- چکیده انگلیسی: Background: The c-Fos nuclear protein dimerizes with Jun family proteins to form the transcription factor AP-1
complex which participates in signal transduction and regulation of normal cellular processes. In tumorigenesis, c-Fos
promotes invasive growth through down-regulation of tumor suppressor genes but its role in oral carcinogenesis is not
clear. Objectives: This study concerned c-fos gene expression in normal and malignant tissues of the oral cavity, with
attention to associations between expression status and clinico-pathological profiles of OSCC patients. Method: A total
of 65 histopathologically confirmed OSCC tissue samples were included in case group along with an equal number of
age and sex-matched normal tissue samples of oral cavity for the control group. c-Fos protein and m-RNA expressions
were analyzed using immunohistochemistry and qRT-PCR, respectively. Results: A significant low expression of c-Fos
protein was observed in OSCC cases than normal control subjects (p= <0.001). The mean percent positivity of c-Fos
protein in cases vs. controls was 24.91± 2.7 vs. 49.68± 2.2 (p= <0.001). Most OSCC tissue samples showed weak or
moderate c-Fos expression whereas 53.8% of normal tissue sections presented with strong immunostaining. Moreover,
the relative m-RNA expression for the c-fos gene was significantly decreased in case group (0.93± 0.48) as compared to
the control group (1.22± 0.87). Majority of c-Fos positive cases were diagnosed with well developed tumor. The mean
percent positivity of c-Fos protein was significantly lower in higher grade tumor as compared with normal oral mucosa
(p= < 0.001). Conclusion: The present study suggested that the c-fos gene is downregulated in oral carcinomas. The
disparity of c-Fos protein levels in different pathological grades of tumor and normal oral tissue samples may indicate
that loss of c-Fos expression is related with the progression of OSCC.- انتشار مقاله: 29-09-1396
- نویسندگان: Akhilesh Krishna,Madan Lal Brahma Bhatt,Vineeta Singh,Shraddha Singh,Pravin Kumar Gangwar,Uma Shankar Singh,Vijay Kumar,Divya Mehrotra
- مشاهده
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: Oral sqamous cell carcinoma (OSCC),Paternally Expressed Gene 10 (PEG10),radiotherapy response,Biomarker
- چکیده:
- چکیده انگلیسی:
Background: Oral squamous cell carcinoma (OSCC) is one of the most common forms of cancer occurring worldwide. PEG10 is well known as a paternally expressed gene from a newly recognized imprinted region at human chromosome 7q21. Previous study had demonstrated that the significant expression of PEG10 was found in radioresistant OSCC cell line and its expression was significantly associated with poor survival in several cancers. Therefore it has been evaluated as a potential marker in OSCC patients undergoing radiotherapy. Objective: This study was conducted to analyze the mRNA expression of PEG10 in OSCC and its expression in relation to clinicpathological features, radiotherapy treatment response and survival. Methods: This study included tissue specimens obtained via biopsy of 118 patients with OSCC who were recommended for radiotherapy treatment and 80 healthy control tissues analysis of mRNA expression of PEG10 was done by real-time quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR). Patients were treated with 70 Gy of radiation dose by shrinking field technique using Cobalt-60 teletherapy machine. Results: Significantly higher mRNA expression of PEG10 was found in OSCC patients when compared with matched controls. High level of PEG10 mRNA expression showed a significant correlation with lymph node metastasis (p = 0.0047) and tumor stage (p = 0.0499). Multivariate Cox regression analysis revealed that high level of mRNA expression of PEG10 was significantly associated with poor survival (p < 0.05). Our research demonstrated that the expression of PEG10 was higher in radioresistant tumor. Conclusion: We observed significantly increased expression of PEG10 in context of lymph node status, advanced stage and poor survival in our study. Thus PEG10 gene can be used as potential predictive and prognostic biomarker in OSCC patients undergoing radiotherapy.- انتشار مقاله: 11-11-1395
- نویسندگان: Shankar Sharan Singh,Rajendra Kumar,Vandana Singh Kushwaha,Madan Lal Brahma Bhatt,Anshuman Singh,Anupam Mishra,Hari Ram,Devendra Parmar,Rajeev Gupta
- مشاهده