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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: International Journal of Advanced Biological and Biomedical Research
- نوع مقاله: Journal Article
- کلمات کلیدی: Bioinformatics analysis,κ-casein,Camels,Transcription factors
- چکیده:
- چکیده انگلیسی: κ-casein is a glycosilated protein in mammalian milk that plays an essential role in the milk micelles. Control of κ-casein expression reflects this essential role, although an understanding of the mechanisms involved lags behind that of the other milk protein genes. Transcriptional regulation, a first mechanism for controlling the development of organisms, is carried out by transcription factors binding sites (TFBSs). We determined the 5’ region for Iranian Dromedaries and bactrianus camel κ-casein gene. We also determine their regulatory TFBSs regions. This region contained 14 potential TFBSs The four of them C/EBP-α, Oct1, MGF/STAT5 and TPB have almost perfectly conserved.
- انتشار مقاله: 09-05-1393
- نویسندگان: A.A. Khabiri,M. Tahmoorespur,M.R. Nassiri,M.H. Sekhavati
- مشاهده
- جایگاه : پژوهشی
- مجله: Archives of Razi Institute
- نوع مقاله: Journal Article
- کلمات کلیدی: Guinea Pig,Cytokine,mRNA expression,FMD,Real-time PCR
- چکیده:
- چکیده انگلیسی: Foot-and-mouth disease (FMD) is a severely contagious viral disease that mainly affects cloven-hoofed
livestock and wildlife. This study quantifies the cytokines mRNA expression of vaccinated guinea pigs with
FMD type O inactivated vaccine. Blood samples were collected from eight guinea pigs at 7 and 28 days after the
first vaccination. Extracted mRNAs were reverse-transcribed into cDNA and analyzed for quantification of IFN-
γ, TNF-α and IL-10 expression using relative real-time PCR assay. Our results showed that all of the genes were
upregulated. The expression of TNF-α and IL-10 genes significantly increased (P<0.05) in day 28th in
comparison to the day 7th post the first vaccination. It can be concluded that the vaccine induced immune
responses by increasing expression of the cytokines. Therefore, effects of DNA vaccines on immune system also
may be evaluated using these genes.- انتشار مقاله: 26-10-1392
- نویسندگان: R. Pasandideh,M.R. Nassiri,M. Raouf Delgosha,A.A. Aslaminejad,M. Tahmoorespur,S. Zibaei,M. Doosti,M. Pasandideh
- مشاهده