در هنگام جستجو کلمه در قسمت عنوان میتوانید کلمات مورد جستجو را با کاراکتر (-) جدا کنید.
کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: International Journal of Advanced Biological and Biomedical Research
- نوع مقاله: Journal Article
- کلمات کلیدی: Bioinformatics analysis,κ-casein,Camels,Transcription factors
- چکیده:
- چکیده انگلیسی: κ-casein is a glycosilated protein in mammalian milk that plays an essential role in the milk micelles. Control of κ-casein expression reflects this essential role, although an understanding of the mechanisms involved lags behind that of the other milk protein genes. Transcriptional regulation, a first mechanism for controlling the development of organisms, is carried out by transcription factors binding sites (TFBSs). We determined the 5’ region for Iranian Dromedaries and bactrianus camel κ-casein gene. We also determine their regulatory TFBSs regions. This region contained 14 potential TFBSs The four of them C/EBP-α, Oct1, MGF/STAT5 and TPB have almost perfectly conserved.
- انتشار مقاله: 09-05-1393
- نویسندگان: A.A. Khabiri,M. Tahmoorespur,M.R. Nassiri,M.H. Sekhavati
- مشاهده
- جایگاه : پژوهشی
- مجله: Archives of Razi Institute
- نوع مقاله: Journal Article
- کلمات کلیدی: Brucella melitensis,Omp25,BLS,Bioinformatics analysis,Recombinant vaccine
- چکیده:
- چکیده انگلیسی: Brucellosis is a well-known infection in domestic animals which caused by Brucella bacterium. Due to
serious economic and medical consequences of this disease, various efforts have been made to prevent the
infection through the use of recombinant vaccines based on Brucella outer membrane protein (OMP)
antigens. The objectives of the present study were cloning, sequencing and epitope prediction of Omp25
and BLS genes as two major Brucella melitensis antigens. The full-length open reading frame (ORF) of
Omp25 and BLS genes were amplified and cloned into pTZ57R/T vector. Phylogenetic analysis of
sequenced genes showed that both genes were nearly similar in different Brucella species. Several online
prediction softwares were used to predict B and T-cells epitopes, secondary and tertiary structures,
antigenicity ability and enzymatic degradation sites. Bioinformatic tools used in the current study were
confirmed by the results of three different experimental epitope predictions. Bioinformatic analysis
identified five and two B-cell and two and one T-cell epitopes for Omp25 and BLS antigens, respectively.
Finally, according to the antigenicity ability and proteosomal recognition site common B and T-cell epitope
was predicted for Omp25 (154-162 amino acids) and BLS (37-48 and 119-139 amino acids). Results of this
study might be useful for recombinant vaccine development.- انتشار مقاله: 05-06-1393
- نویسندگان: M. Tahmoorespur,M.H. Sekhavati,S. Yousefi,T. Abbassi-Daloii,M. Azghandi,R. Akbari
- مشاهده