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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: Archives of Razi Institute
- نوع مقاله: Journal Article
- کلمات کلیدی: Guinea Pig,Cytokine,mRNA expression,FMD,Real-time PCR
- چکیده:
- چکیده انگلیسی: Foot-and-mouth disease (FMD) is a severely contagious viral disease that mainly affects cloven-hoofed
livestock and wildlife. This study quantifies the cytokines mRNA expression of vaccinated guinea pigs with
FMD type O inactivated vaccine. Blood samples were collected from eight guinea pigs at 7 and 28 days after the
first vaccination. Extracted mRNAs were reverse-transcribed into cDNA and analyzed for quantification of IFN-
γ, TNF-α and IL-10 expression using relative real-time PCR assay. Our results showed that all of the genes were
upregulated. The expression of TNF-α and IL-10 genes significantly increased (P<0.05) in day 28th in
comparison to the day 7th post the first vaccination. It can be concluded that the vaccine induced immune
responses by increasing expression of the cytokines. Therefore, effects of DNA vaccines on immune system also
may be evaluated using these genes.- انتشار مقاله: 26-10-1392
- نویسندگان: R. Pasandideh,M.R. Nassiri,M. Raouf Delgosha,A.A. Aslaminejad,M. Tahmoorespur,S. Zibaei,M. Doosti,M. Pasandideh
- مشاهده