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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: Desert
- نوع مقاله: Journal Article
- کلمات کلیدی: Yield,salt stress,Ions,Populus euphratica
- چکیده:
- چکیده انگلیسی: Euphrates poplar (Populus euphratica Oliv.) is a woody species that is naturally distributed in the desert areas of some parts of Asia and Africa. Because of its outstanding features, it is a model plant to study environmental stress tolerance. This research was conducted from 2014 to 2016 in order to study the relationship between performance indices and ion concentrations. The cuttings of 12 ecotypes were collected from different climatic conditions in Iran. Salinity stress was applied using four levels of NaCl (75, 150, 225 and 300 mM) and one control sample (salt-free). The performance indices [diameter and height growth, biomass production; leaf, stem, root and total biomass] showed significant differences in salt levels and ecotypes. The ion concentrations showed significant differences in salt levels (except Ca2+) and varied in different ecotypes. There was no significant difference in salt×ecotype interaction for most of the variables. The ecotypes, treatments, (salt levels) means of performance indices and ion concentrations were separated into different groups. Correlation coefficients showed that the concentration of macronutrients had positive correlations with performance indices, and that salt ions had negative correlations. Correlation coefficients also showed that the ion concentrations had synergistic or antagonistic effects on each other. The results of this study showed that the key mechanisms of salt tolerance in this specie include: exclusion of salt from the root, compartmentalization of Na+ in plant tissue, preventing excessive reduction of K+ absorption resulting in the maintenance of the K+/Na+ balance.
- انتشار مقاله: 30-01-1396
- نویسندگان: H. Tavakoli Neko,A. Shirvany,M.H. Assareh,M.R. Naghavi,M. Pessarakli,A. Pourmeidani
- مشاهده
- جایگاه : پژوهشی
- مجله: Archives of Razi Institute
- نوع مقاله: Journal Article
- کلمات کلیدی: Cytokines,Brucella melitensis,Interleukin,Omp31
- چکیده:
- چکیده انگلیسی: Brucellosis is a zoonotic disease in human and animals. Brucella melitensis is one of the most pathogenic species of Brucella in goat and sheep. Omp31 is an outer membrane protein of Brucella that acts as an immunogenic protein. Cytokines are glycoproteins with low molecular weight that play the role of an immune adjuvant and regulate immune responses. Interleukin-2 is one of the most important cytokines, which are secreted by the white blood cells and involved in T cell immune responses. In the present study, a chimeric Omp31-Interleukin2 recombinant protein was generated by means of genetic engineering techniques. This chimeric coding sequence was amplified by using specific primers and using Splicing Overlap Extension (SOE) PCR technique. The fusion of the two mentioned proteins was accomplished using a rigid linker. The generated chimeric IL2-Omp31 fragment was TA cloned, and then subcloned into pEt22b vector as an expression vector. The chimeric protein was successfully expressed in E. coli BL21 (DE3) and confirmed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and also Western-blotting analysis. Finally, in order to assess the antigenic features of the recombinant chimeric IL2-Opm31 protein, its secondary structure and antigenicity were predicted in silico.
- انتشار مقاله: 17-02-1396
- نویسندگان: M. Naghavi,M. H. Sekhavati,M. Tahmoorespur,M. R. Nassiri
- مشاهده