در هنگام جستجو کلمه در قسمت عنوان میتوانید کلمات مورد جستجو را با کاراکتر (-) جدا کنید.
کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: Iranian Journal of Immunology
- نوع مقاله: Journal Article
- کلمات کلیدی: MSCs,Immunomodulation,DCs
- چکیده:
- چکیده انگلیسی: Background: Mesenchymal stem cells (MSCs) possess a wide range of immunomodulatory functions mostly in immune cells including dendritic cells (DCs). DCs are the key cells in the immune response and play an important role in initiating cell-mediated immunity.
Objective: To evaluate the immunomodulatory effects of MSCs supernatant on maturation and function of DCs.
Methods: Bone marrow derived mice MSCs were isolated and cultured. Twenty-four, forty-eight and seventy-two hours after passage 6, supernatants were collected and MSCs were assessed by cytometric analysis for the expression of CD34, CD44, CD45 and SCA-1. Splenic DCs were isolated using MACS and then co-cultured with MSCs supernatant. Expression of CD86, CD40 and MHC-II on DCs were also evaluated by cytometry. H 3-thymidine incorporation by proliferating T cells was determined in two separate MLR assay settings. In one setting, DCs were co-cultured with T cells in the presence of MSCs supernatant, and in the other setting DCs were treated with MSCs supernatant and then were co-cultured with T cells. Production of IL-12, IL-6 and IL-10 cytokines was measured in the supernatant of DCs treated with MSCs supernatant. We also measured IFN- γ and IL-4 levels in MLR supernatant.
Results: The results showed that 72h MSCs supernatant could decrease the expression of MHC-II and CD86. The T cell proliferation was inhibited in the presence of MSCs supernatant and MSCs supernatant treated DCs as demonstrated by MLR assay. A significant increase in IL-4 level and a non significant decrease in IFN- γ level in MLR supernatant were observed. However, IL-6, IL-10 and IL-12 production did not change significantly.
Conclusion: MSCs supernatant has a time dependent effect on the maturation of DCs. Also, it could alter cytokine production from responding T cells toward Th2. Generally, the findings of this study supported the immunomodulatory effect of MSCs supernatant on DCs maturation and function.- انتشار مقاله: 15-05-1395
- نویسندگان: Ladan Sadeghi,Eskandar Kamali-Sarvestani,Negar Azarpira,Mehrdad Shariati,Mohammad Hossein Karimi
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Immunology
- نوع مقاله: Journal Article
- کلمات کلیدی: Hsp60,CRP,Ischemic Heart Disease,Chlamydia pneumoniae
- چکیده:
- چکیده انگلیسی: Background: Inflammation and infectious agents such as Chlamydia pneumoniae have been associated with cardiovascular disease.
Objective: To evaluate the serum high sensitivity C - reactive protein (hs-CRP) and antibodies against Chlamydia pneumoniae and Chlamydial heat shock protein-60 (Cp-HSP60) in patients with ischemic heart disease (IHD).
Methods: 62 patients with IHD having either acute myocardial infarction (AMI; n=31) or unstable an-gina (UA; n=31) and 31 sex- and age- matched healthy subjects as a control group were en-rolled in this study. Serum samples of participants were tested for the presence of hs-CRP and antibodies against C. pneumoniae and Cp-HSP60 using ELISA method.
Results: The sero-prevalence of anti-C. pneumoniae antibody in AMI group (93.5%) or UA group (90.3%) was significantly higher than the control group (61.3%; p<0.001). The sero-prevalence of anti-Cp-HSP60 IgG was 22.6% in healthy subjects with mean end titer of 43.1 ± 6.32. The seropositive rates of anti-Cp-HSP60 were 48.4%, 54.8% and 51.6% in AMI, UA and the overall IHD groups with mean end titers of 94 ± 22.86, 113.8 ± 24.25 and 103.9 ± 16.57, respectively. Both the seroprevalence and the mean titer of anti-Cp-HSP60 in patients groups were significantly higher than those observed in the control group (p<0.04 and p<0.03, respectively). Moreover, the mean serum hs-CRP levels was significantly higher in the IHD group as compared to the control group (21.6 μg/ml ± 3.73 vs 2.5 μg/ml ± 0.52; p<0.00001). The mean serum hs-CRP levels of AMI (30.3 μg/ml ± 6.07) or UA (12.9 μg/ml ± 3.85) groups were also significantly higher than those observed in the control group (p<0.00001 and p<0.001, respectively). Further-more, the difference of the mean serum hs-CRP levels between AMI and UA groups was also significant (p<0.02).
Conclusions: These results showed that the seroprevalence of antibodies against C. pneumoniae and Cp-HSP60 and the serum levels of hs-CRP and anti-Cp-HSP60 IgG were higher in patients with IHD.- انتشار مقاله: 16-05-1395
- نویسندگان: Abdollah Jafarzadeh,Ali Esmaeeli-Nadimi,Mehdi Shariati
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of English for Academic Purposes
- نوع مقاله: Journal Article
- کلمات کلیدی: Reading Comprehension,EFL Learners,Vocabulary Retention,Multimodality
- چکیده: هدف از این تحقیق بررسی تاثیر روش چندوجهی بر درک مطلب و یادگیری لغت در بین زبان آموزان ایرانی میباشد. نمونه آماری این تحقیق را 30 زبان آموز مذکر آموزشگاه زبان انگلیسی رازی شهرستان انار تشکیل میدهد که پس از برگزاری آزمون تعیین سطح آکسفورد به صورت تصادفی به دو گروه آزمایشی و کنترل تقسیم شدند. اطلاعات جمع آوری شده با استفاده از روش آماری تجزیه و تحلیل چندمتغیره کواریانس مورد تجزیه و تحلیل قرار گرفت و نتایج مرحله کمی این تحقیق نشان داد که استفاده از روش چندوجهی بر درک مطلب و یادگیری لغت زبان آموزان تاثیر مثبت دارد. همچنین در مرحله کیفی تحقیق نظرات و عقاید شرکت کنندگان گروه آزمایشی در ارتباط با تاثیر وجه تصویر، ویدئو یا صدا در درک مطلب، وضعیت کلاسهای درسی زبان آموزان، متون چندوجهی و ارتقاء سطح زبانی آنان از طریق پرسشنامه و مصاحبه مورد بررسی قرار گرفت. نتایج مرحله کیفی تحقیق نشان داد که زبان آموزان متونی را که با تصویر همراه است به سایر متون ترجیح میدهند. همچنین نتایج این مرحله از تحقیق نشان داد زبان آموزان از تکنولوزی و متون چندوجهی برای یادگیری، سرگرمی و ارتباطات استفاده میکنند. زبان آموزان بر این باور بودند که متون چندوجهی بر پیشرفت سطح زبانی آنان تاثیر دارد و آنها استفاده از روش متون چندوجهی را بر روش متون سنتی ترجیح میدهند. همچنین زبان آموزان اعتقاد داشتند که معلمان زبان باید از روش چندوجهی برای تدریس استفاده کنند در حالی که آنها از این روش برای تدریس در کلاس درس استفاده نمیکنند.
- چکیده انگلیسی: The studies that have investigated the effects of multimodality on reading comprehension and vocabulary retention of EFL learners in the context of Iran through mixed methodology are very limited. Accordingly, this two-phase study aimed to investigate the effect multimodalities might have on reading comprehension and vocabulary retention of Iranian EFL learners. To this end, the first phase of the study included a sample of 30 male EFL learners selected through intact group sampling who were homogenized by using an OPT (Oxford Placement Test). Then they divided into experimental and control groups randomly. Passages used for experimental group were accompanied by visual images, videos, and audio tracks, while passages used for control group were the same texts without any of these. For conducting the second phase of the study, in the control group the teacher introduced the new vocabularies of each text only by their L2 definition but in the experimental group the teacher used L2 definition and image for introducing the new vocabularies. In the qualitative part of the study, an open-ended questionnaire and interview were used. By comparing pre-tests and post-tests using MANCOVA, the results showed the effectiveness of using multimodality in L2 reading classrooms and vocabulary retention of EFL learners. The analysis of the results obtained from the qualitative phase revealed that learners preferred texts to be accompanied by visual images.
- انتشار مقاله: 13-07-1398
- نویسندگان: Nahid Naderi Anari,Ali Asghar Rostami Aboo Saeedi,Mohammad Shariati
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Basic Medical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: Vancomycin,Drug Delivery,MRSA,Nanopliposomes,Endophthalmitis
- چکیده:
- چکیده انگلیسی: Objective(s): The in vivo efficacy of nanoliposomal formulation of vancomycin against methicillin-resistant Staphylococcus aureus (MRSA) assessed.
Materials and Methods: Nanoliposomal formulations were prepared and characterized. The in vivo study was carried out on rabbits which received liquid culture medium containing MRSA under anesthesia. After 48 hr, the eyes treated with the liposomal and free form of vancomycin. The rabbits were euthanized at predesignate intervals at 12, 24, 48, 96, 144 hr intervals injection. The antibacterial activity of different vancomycin formulations was assayed by the time killing method.
Results: The zeta potential, mean sizes and encapsulation efficacy of liposomal vancomycin were 29.7 mV, 381.93±30.13 nm and 47%, respectively. The results of time–killing studies indicated that the liposomal formula was more effective than the free form of vancomycin.
Conclusion: The results of this study revealed that liposomal vancomycin formulation is a powerful nano-antibacterial agent to combat infectious endophthalmitis.- انتشار مقاله: 05-07-1398
- نویسندگان: Majid Abrishami,Mehrdad Motamed Shariati,Bizhan Malaekeh-Nikouei,Amineh Sadat Tajani,Asma Mahmoudi,Mojtaba Abrishami,Bahman Khameneh
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Basic Medical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: Vancomycin,Drug Delivery,MRSA,Nanopliposomes,Endophthalmitis
- چکیده:
- چکیده انگلیسی: Objective(s): The in vivo efficacy of nanoliposomal formulation of vancomycin against methicillin-resistant Staphylococcus aureus (MRSA) assessed.
Materials and Methods: Nanoliposomal formulations were prepared and characterized. The in vivo study was carried out on rabbits which received liquid culture medium containing MRSA under anesthesia. After 48 hr, the eyes treated with the liposomal and free form of vancomycin. The rabbits were euthanized at predesignate intervals at 12, 24, 48, 96, 144 hr intervals injection. The antibacterial activity of different vancomycin formulations was assayed by the time killing method.
Results: The zeta potential, mean sizes and encapsulation efficacy of liposomal vancomycin were 29.7 mV, 381.93±30.13 nm and 47%, respectively. The results of time–killing studies indicated that the liposomal formula was more effective than the free form of vancomycin.
Conclusion: The results of this study revealed that liposomal vancomycin formulation is a powerful nano-antibacterial agent to combat infectious endophthalmitis.- انتشار مقاله: 05-07-1398
- نویسندگان: Majid Abrishami,Mehrdad Motamed Shariati,Bizhan Malaekeh-Nikouei,Amineh Sadat Tajani,Asma Mahmoudi,Mojtaba Abrishami,Bahman Khameneh
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Basic Medical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: Kidney,Lung,Fluoxetine,Heart,SSRIs
- چکیده:
- چکیده انگلیسی: Objective(s): Depression during pregnancy negatively affects fetal development. Fluoxetine as a selective serotonin reuptake inhibitor (SSRIs) is used for treatment of gestational depression. This study is trying to determine the effects of fluoxetine on the renal, heart and lung development.
Materials and Methods: Fifteen pregnant rats were treated with fluoxetine at 7 mg/kg from days 0 to 21 of gestation. Immediately after born, heart and kidney samples were evaluated for genes expression and histological assessment. Lung sample were fixed for immunohistochemical study.
Results: The gene expression of BMP7 and WNT4 were reduced in the kidney of fluoxetine-treated group (P-value<0.05), but in the heart of both groups no significant difference was found in gene expression (P-value>0.05). Histological assessment showed that the glomeruli of the kidneys in treated group are more primordial compared to control. There was a developmental deficiency in Bowman’s capsule, and the capsular space was not clear. The arrangements of the filaments, the position of the nucleus and cells morphology were normal in the hearts of both groups. Immunohistochemical analysis demonstrated that in the fluoxetine-exposed group HoxB5 is more expressed in the mesenchymal cells, but in the control group the expression is limited to alveolar cells.
Conclusion: According to developmental changes in kidney, heart and lung, fluoxetine affects neonatal growth during pregnancy, which may lead to delay of some organs growth. So, it is essential to survey the roles of antidepressant drugs on fatal and neonatal development during pregnancy.- انتشار مقاله: 04-08-1396
- نویسندگان: Razieh Taghizadeh Ghvamabadi,Zahra Taghipour,Mahsa Hasanipoor,Marzieh Khademi,Mehdi Shariati
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Basic Medical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: Inflammation,TLR4,TLR2,Cerebral ischemia,TRPV1
- چکیده:
- چکیده انگلیسی: Objective(s): Stroke is known as a main cause of mortality and prolonged disability in adults. Both transient receptor potential V1 (TRPV1) channels and toll-like receptors (TLRs) are involved in mediating the inflammatory responses. In the present study, the effects of TRPV1 receptor activation and blockade on stroke outcome and gene expression of TLR2 and TLR4 were assessed following permanent middle cerebral artery occlusion in rats
Materials and Methods: Eighty male Wistar rats were divided into four groups as follows: sham, vehicle, AMG9810 (TRPV1 antagonist) -treated and capsaicin (TRPV1 agonist) -treated. For Stroke induction, the middle cerebral artery was permanently occluded and then behavioral functions were evaluated 1, 3 and 7 days after stroke.
Results: TRPV1 antagonism significantly reduced the infarct volume compared to the stroke group. Also, neurological deficits were decreased by AMG9810 seven days after cerebral ischemia. In the ledged beam-walking test, the slip ratio was enhanced following ischemia. AMG9810 decreased this index in stroke animals. However, capsaicin improved the ratio 3 and 7 days after cerebral ischemia. Compared to the sham group, the mRNA expression of TLR2 and TLR4 was significantly increased in the stroke rats. AMG9810 Administration significantly reduced the mRNA expression of TLR2 and TLR4. However, capsaicin did not significantly affect the gene expression of TLR2 and TLR4.
Conclusion: Our results demonstrated that TRPV1 antagonism by AMG9810 attenuates behavioral function and mRNA expression of TLR2 and TLR4. Thus, it might be useful to shed light on future therapeutic strategies for the treatment of ischemic stroke.- انتشار مقاله: 14-05-1396
- نویسندگان: Elham Hakimizadeh,Ali Shamsizadeh,Ali Roohbakhsh,Mohammad Kazemi Arababadi,Mohammad Reza Hajizadeh,Mehdi Shariati,Iman Fatemi,Amir Moghadam-ahmadi,Gholamreza Bazmandegan,Hossein Rezazadeh,Mohammad Allahtavakoli
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Basic Medical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: Learning,Morris Water Maze,Leptin resistance,Losartan,Memory performance
- چکیده:
- چکیده انگلیسی:
Objective(s):
Leptin is a hormone secreted by adipose tissue and is involved not only in the regulation of feeding and energy expenditure, but also its role in memory enhancement has been demonstrated as well. The partial transfer of leptin across the blood-brain barrier in obese individuals causes leptin resistance and prevents leptin reaching brain. On the other hand, studies have shown that angiotensin antagonists such as losartan can improve memory and learning abilities. The aim of this study was to evaluate the effects of losartan on improving memory and leptin resistance induced by high fat diet in obese rats.
Materials and Methods:
40 Wistar male rats were divided in 4 groups: control (C), losartan (LOS), high-fat diet (HFD) and high-fat diet and losartan (HFD and LOS). The spatial memory performances of the rats were assessed in the Morris water maze after 2 months of treatment. Then they were weighed and serum levels of leptin and triglyceride were measured.
Results:
In spite of receiving high-fat diet, no significant differences in body weight were observed in the (HFD & LOS) group. In the Morris water maze trial, the (LOS) and (HFD & LOS) groups also showed a significant reduction (P <0.05) in latency and path length. In addition, a significant decrease (P
<0.05) in serum levels of leptin and no significant difference in serum levels of triglyceride was observed in the (HFD & LOS) group.
Conclusion:
Losartan can improve leptin resistance induced by obesity and high fat diet. At the same time, it modulates body weight and enhances learning and memory.- انتشار مقاله: 05-11-1392
- نویسندگان: Seyydeh Gohar Sharieh Hosseini,Saeed Khatamsaz,Mehrdad Shariati
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Biotechnology
- نوع مقاله: Journal Article
- کلمات کلیدی: Schizophrenia,ARMS-PCR,Neuregulin1,SNP8NRG221533
- چکیده:
- چکیده انگلیسی: Schizophrenia is a severe neuropsychiatric disorder with symptoms such as hallucination, delusion and mental disorder. It is a complex disorder, in which genetic components play a crucial role in its pathogenesis. Among candidate genes for schizophrenia, Neuregulin 1 (NRG1) gene is the most important gene, association of which with the illness has been confirmed in several studies. Single nuclotide polymorphisms (SNPs) located 5´ upstream of NRG1 have shown significant association with schizophrenia in several populations. Here, we describe a designed simple Multiplex Tetra-Primer Amplification Refractory Mutation System - polymerase chain reaction (PCR) for genotyping single SNP (SNP8NRG221533) in the human NRG1 gene. No restriction site was found for distinguishing T and C alleles of this SNP. The developed method proved to be simple, rapid and cost effective. This technique was used to compare SNP8NRG221533 in 95 schizophernics and 95 healthy controls. Our data demonstrate that there is a significant difference between allelic and genotypic frequencies of the two groups. These preliminary results confirm the association of the NRG1 gene with schizophrenia in an Iranian population.
- انتشار مقاله: 06-02-1394
- نویسندگان: Seyed Ali Mohammad Shariati,Mehrdad Behmanesh,Hamid Galehdari,Ali Fathian
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Chemistry and Chemical Engineering
- نوع مقاله: Journal Article
- کلمات کلیدی: Response surface methodology,Hydrogenation,Fullerene,Aniline,Ruthenium,Nitrobenzene
- چکیده:
- چکیده انگلیسی: In this study fullerene functionalized using oleum (H2SO4·SO3), followed by the hydrolysis of the intermediate cyclosulfated fullerene as well as an oxidizing agent was employed to functionalize the fullerenes. Ruthenium was then added by the impregnation method or deposited on the functionalized fullerene. Subsequent to this step, Response Surface Methodology (RSM) was used to study the cumulative effect of various parameters including, pressure, temperature, time and loading. In order to maximize the hydrogenation of nitrobenzene (NB) to aniline (AN) these latter parameters were optimized. Furthermore, catalytic activity was evaluated over a temperature range of 25–150°C, hydrogen pressure of 1-30 atm, ruthenium content of 1-15%(w/w) and reaction time of 30-180 min in a bench scale reactor. The optimized model predicted that the hydrogenation should be at a maximum level (approximately 100%) with the following conditions; Ru loading of 15%, reaction temperature of 150 °C, reaction time of 180 min and hydrogen pressure of 22.33 atm.
- انتشار مقاله: 26-04-1392
- نویسندگان: Hassan Keypour,Mohammad Noroozi,Alimorad Rashidi,Masoud Shariati Rad
- مشاهده