در هنگام جستجو کلمه در قسمت عنوان میتوانید کلمات مورد جستجو را با کاراکتر (-) جدا کنید.
کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: Journal of Advances in Environmental Health Research
- نوع مقاله: Journal Article
- کلمات کلیدی: Gold nanoparticles,Wastewater,Enterococci,Vancomycin
- چکیده:
- چکیده انگلیسی: Industrial wastewater is one of the most dangerous and important sources of bacterial pathogens. This study aimed to determine the frequency of vancomycin-resistant enterococci (VRE) in samples taken from wastewater plants of Golestan Province, Iran, and evaluate the antimicrobial effect of gold nanoparticles (AuNPs) in combination with vancomycin on the growth of isolates resistant to vancomycin. Samples were taken from three plants in Gorgan, Kordkuy and Bandar Turkoman. Enterococcal species were identified based on the most probable number (MPN), filtration, microbiological and biochemical tests. Susceptibility to six antibiotics with monitoring of vancomycin was investigated using the Kirby-Bauer method, according to the CLSI-2015 guidelines. The antibacterial effect of AuNPs was evaluated using agar well diffusion method. More than 60% of wastewater samples were positive for enterococcal species, 65% of which were found in raw effluent, while the remaining 35% were found in the treated effluent. Based on the results, 88.2% of the isolates were resistant to ampicillin. The frequency of vancomycin-resistant enterococci was 47.1%. Our findings indicate the presence of multi-drug resistant enterococci and high rate of vancomycin resistance in wastewater samples from the Golestan Province, Iran. Results show good antibacterial effects of AuNp s in combination with vancomycin in high densities against all the drug-resistant enterococci strains.
- انتشار مقاله: 26-03-1399
- نویسندگان: Niayesh Enayatimoghaddam,Leila Fozouni,Ania Ahani Azari
- مشاهده
- جایگاه : پژوهشی
- مجله: Journal of Advances in Environmental Health Research
- نوع مقاله: Journal Article
- کلمات کلیدی: Gold nanoparticles,Wastewater,Enterococci,Vancomycin
- چکیده:
- چکیده انگلیسی: Industrial wastewater is one of the most dangerous and important sources of bacterial pathogens. This study aimed to determine the frequency of vancomycin-resistant enterococci (VRE) in samples taken from wastewater plants of Golestan Province, Iran, and evaluate the antimicrobial effect of gold nanoparticles (AuNPs) in combination with vancomycin on the growth of isolates resistant to vancomycin. Samples were taken from three plants in Gorgan, Kordkuy and Bandar Turkoman. Enterococcal species were identified based on the most probable number (MPN), filtration, microbiological and biochemical tests. Susceptibility to six antibiotics with monitoring of vancomycin was investigated using the Kirby-Bauer method, according to the CLSI-2015 guidelines. The antibacterial effect of AuNPs was evaluated using agar well diffusion method. More than 60% of wastewater samples were positive for enterococcal species, 65% of which were found in raw effluent, while the remaining 35% were found in the treated effluent. Based on the results, 88.2% of the isolates were resistant to ampicillin. The frequency of vancomycin-resistant enterococci was 47.1%. Our findings indicate the presence of multi-drug resistant enterococci and high rate of vancomycin resistance in wastewater samples from the Golestan Province, Iran. Results show good antibacterial effects of AuNp s in combination with vancomycin in high densities against all the drug-resistant enterococci strains.
- انتشار مقاله: 26-03-1399
- نویسندگان: Niayesh Enayatimoghaddam,Leila Fozouni,Ania Ahani Azari
- مشاهده
- جایگاه : پژوهشی
- مجله: International Journal of Molecular and Clinical Microbiology
- نوع مقاله: Journal Article
- کلمات کلیدی: Drug resistance,Staphylococcus aureus,nanoparticle,Zinc oxide,Vancomycin
- چکیده:
- چکیده انگلیسی: Staphylococcus aureus, as one of the main agentsfor hospital infections,is considered as highly important because they show resistance to a wide range of antibiotics.Resistance to selective antibiotics such as vancomycin is a serious problemin the medical community; thus it seems rational to use alternative substances for treating these bacteria.The aim of this study isto investigate the prevalence of resistance to vancomycin in clinical isolates of Staphylococcus aureus as well as theantimicrobial effects of zinc oxidenanoparticles on them. In this study, 70 samples of wound, boil, abscess and urine were isolated.Staphylococcus aureus strains resistant to vancomycin were then identified through routine laboratory tests using Broth Microdilution test. The antibacterial effect of ZnO nanoparticles(20 nanometer) was investigated at concentrations of 100,50,25,12.5 and 6.25 mg/ml using Agar well diffusion method over strains resistant to vancomycin. From the total of 70 samples, 30 samples were identified as Staphylococcus aureus out of which 23.3% showed resistance to vancomycin. During this study it was found that ZnO nanoparticles in concentrations of 50 and 100 mg/ml have a good antibacterial effect and can be a good alternative for controlling Staphylococcus aureus resistant to vancomycin. Considering the increasing trend in drug resistance, the growth of pathogenic bacteria can be inhibited by increasing the concentration of zinc oxide nanoparticles.
- انتشار مقاله: 04-06-1395
- نویسندگان: Fahimeh Esfahani,Leila Fozouni,Hamidreza Pordeli
- مشاهده
- جایگاه : پژوهشی
- مجله: International Journal of Molecular and Clinical Microbiology
- نوع مقاله: Journal Article
- کلمات کلیدی: Candida albicans,MIC,Eucalyptus essence,Lavandula essence
- چکیده:
- چکیده انگلیسی: Candida albicans is the most common and important factor causing candidiasis infections in human. As fungi are resistant to anti-fungal agents, and also with respect to the restrictions in treatment of fungal diseases such as their high costs and side effects, study of the combination of herbal medicine is truly required. The aim of the present study was to investigate the effects of herbal essences of eucalyptus and lavandula on the growth of clinical Candida albicans strains which have been separated from mucosal, cutaneous and visceral infections, and compared to those Candida albicans which are resistant and sensitive to caspofungin. The present study has been carried out on 41 Candida albicans taken from patients suffering from mucosal, cutaneous and visceral candidiasis. The degree of samples’ sensitivity to eucalyptus and lavandula were determined through applying broth microdilution method. In the present study, the MIC (minimum inhibitory concentration) of eucalyptus against Candida albicans was determined at 4-1024µl/ml, in which the most growth fluctuations were seen in the densities of 512 and 128 µl/ml. Moreover, the MIC of lavandula was determined at 1-256 µl/ml, and the most fluctuations in its growth were in the density of 32 and 16 µl/ml. Eucalyptus and lavandula essences have a very good anti-fungal effect against Candida albicans. In the present study, lavandula essence proved better anti-candidiasis effects while no considerable difference was seen between the strains sensitive and resistant to caspofungin in its MIC. In higher densities, eucalyptus essence prevents strains from growing. Therefore, it is recommended to investigate the effects of these essences on other pathogenic fungi and bacteria. It is also recommended to evaluate the use of combinations of two or more essences for curing infections or preventing their reoccurrence.
- انتشار مقاله: 01-03-1391
- نویسندگان: Leila Fozouni,Mansour Bayat,Shahla Roudbar Mohammadi,Mohammad Hassan Shahhosseiny
- مشاهده
- جایگاه : پژوهشی
- مجله: International Journal of Hospital Research
- نوع مقاله: Journal Article
- کلمات کلیدی: Drug resistance,Pseudomonas aeruginosa,Burn,Probiotic,Antagonistic effect
- چکیده:
- چکیده انگلیسی: Background and Objectives: Pseudomonas aeruginosa is an opportunistic bacterium and is one of the main causes of infection in burn patients. As this bacterium gets resistant to various types of antibiotics through different acquiring mechanisms, it can easily induce skin deformities and even patients’ death.
Methods: This descriptive study was carried out on 200 patients with burn injuries hospitalized in specialized burn hospitals. Culture methods and diagnosis tests were used to separate and diagnose P. aeruginosa, and disk diffusion Agar method with the Kirby-Bauer standard was applied to determine the pattern of drug-resistance. The antagonist effect of lactic bacteria isolated from 14 samples of milk and yogurt on the growth of P. aeruginosa was also assessed using Agar well diffusion method.
Findings: Out of 30 strains diagnosed and isolated as P. aeruginosa 45.2% were resistant to gentamicin, 51.6% to cefotaxime, 48.8 to imipenem, and 45.2 to ciprofloxacin. Study of probiotics revealed that Lactobacillus casei, with the average diameter of 20.3 mm, has the highest inhibitory effect against P. aeruginosa.
Conclusions: The results of this study showed that the spread of P. aeruginosa resistant to medicine is very high in the surveyed hospitals, and that the isolated lactobacilli have a significant inhibitory effect on drug resistant P. aeruginosa strains. Our results hence suggest that, using probiotic products would help better control of P. aeruginosa infection in burn patients.- انتشار مقاله: 25-03-1395
- نویسندگان: Mohadeseh Abasgholizade,Leila Fozouni
- مشاهده