در هنگام جستجو کلمه در قسمت عنوان میتوانید کلمات مورد جستجو را با کاراکتر (-) جدا کنید.
کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: Iranian Journal of Basic Medical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: Rat,adenine,Nuclear factor erythroid-2-related factor 2,Ozone therpy,Tubuleinterstitial injury
- چکیده:
- چکیده انگلیسی: Objective(s): This study aims to determine the effects of ozone therapy on restoring impaired Nrf2 activation to ameliorate chronic tubulointerstitial injury in rats with adenine-induced CKD.
Materials and Methods: Sprague–Dawley rats were fed with 0.75% adenine-containing diet to induce CKD and chronic tubulointerstitial injury. Ozone therapy was administered by rectal insufflation. After 4 weeks, serum and kidney samples were collected and analyzed. Renal function and systemic electrolyte level were detected. Pathological changes in kidney were assessed by hematoxylin–eosin staining and Masson trichrome staining. Nrf2 activation was detected by immunohistochemistry and Western blot analyses. The levels of SOD, CAT, GSH, PCO, and MDA were detected in the kidney. Immunohistochemistry, Western blot, and real-time PCR analyses were performed to evaluate the activation of the nuclear factor kappa B (NF-κB) P65 pathway and inflammation infiltration in the tubulointerstitium of the rats.
Results: Ozone therapy improved severe renal insufficiency and tubulointerstitial morphology injury as well as restored Nrf2 activation and inhibited the NF-κB pathway in rats with adenine-induced CKD. Ozone therapy also up-regulated anti-oxidation enzymes (SOD, CAT, and GSH) and down-regulated oxidation products (PCO and MDA), as well as inflammatory cytokines (IL-1β, IL-6, TNF-α, and ICAM-1) in the kidney.
Conclusion:These findings indicated that ozone therapy could attenuate tubulointerstitial injury in rats with adenine-induced CKD by mediating Nrf2 and NF-κB.- انتشار مقاله: 02-08-1395
- نویسندگان: Gang Yu,Xiuheng Liu,Zhiyuan Chen,Hui Chen,Lei Wang,Zhishun Wang,Tao Qiu,Xiaodong Weng
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Chemistry and Chemical Engineering
- نوع مقاله: Journal Article
- کلمات کلیدی: Gold,Acetylene hydrochlorination,Mercury-free catalyst,La,multi-tubular fixed bed reactor
- چکیده:
- چکیده انگلیسی: The metal chloride of LaCl3 was chosen to modify the Au-Cu/AC to decrease the noble metal of gold and enhance the catalytic performances. Then a mercury-free catalyst of Au-Cu-La/AC was prepared by the impregnation method, and the fresh Au-Cu-La/AC and Au-Cu/AC catalysts were also characterized in comparison. The catalytic performances of mercury-free catalysts for acetylene hydrochlorination were carried out for 3500 hours in a multi-tubular fixed bed reactor. The additives of La can make the active species dispersed well and retard the aggregation of particles. And the acetylene conversion rate remained stable over 98.5% with the fluctuations, less than 1%, and the selectivity of vinyl chloride maintained the stability of 99% or higher, which indicated that the mercury-free catalyst has excellent catalytic performances for acetylene hydrochlorination.
- انتشار مقاله: 10-03-1397
- نویسندگان: Lei Wang,Benxian Shen,Jigang Zhao,Chunlei Wu,Xiaotao Bi
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Chemistry and Chemical Engineering
- نوع مقاله: Journal Article
- کلمات کلیدی: Melamine,DFT calculation,Bimetallic catalyst,Acetylene hydrochlorination
- چکیده:
- چکیده انگلیسی: This paper highlights the experimental and theoretical studies on the Melamine treated Active Carbon (MAC) support for an Au-Cu bimetallic catalyst in acetylene hydrochlorination reaction. Compared to the original Active Carbon (AC) loaded with the same amount of 0.1wt% Au and 1.0wt% Cu, MAC supported catalyst(MACH), wherein Carbon/C6H6N6 mass ratio was 5:3, exhibited excellent catalytic activity. The initial conversion of acetylene increased from 77.5% to 82.7% at 150℃ and atmospheric pressure. The gas hourly space velocity (GHSV) was 120h-1 under a feed volume ratio VHCl/VC2H2 of 1.05. As polymerization of acetylene on the catalyst was the main cause of deactivation, accelerated deactivation test was carried out. The result indicated MACH performed good anti-coking capacity. Based on the characterization by using BET, XRD, SEM, TGA, TPD and XPS techniques, the variation of O1s XPS spectra of the synthesized catalysts was observed that was in line with DFT results. It is postulated that the better stability and the better dispersion of gold actions were ascribed to the additional metal and the modified substrate. The electron transmission from the auxiliary and the elevated groups on the catalyst surface partially inhibited the reduction of the Au3+ active species. Meanwhile, the stronger adsorption energy of HCl was also beneficial to catalytic activity and stability.
- انتشار مقاله: 30-05-1395
- نویسندگان: Lei Wang,Jigang Zhao,Benxian Shen,Yehui Zhang,Chunlei Wu
- مشاهده
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: Hepatocellular carcinoma,Autophagy,Key words: Sorafenib,cis-platinum
- چکیده:
- چکیده انگلیسی: Objective: To explore the effect of combined Sorafenib/ cisplatinum treatment on the autophagy and proliferation of hepatocellular carcinoma (HepG2) cells in vitro. Methods: HepG2 cells were cultured and treated with different concentrations of Sorafenib, cisplatinum, or a combination of both over a 24-hour period. Cell proliferation was evaluated using a CCK8 assay, and the mRNA expression of the autophagy-related proteins AKT, mTOR, and LC3 were detected using quantitative PCR (qPCR). AKT, pAKT (Ser473), mTOR, pmTOR (Ser2448), LC3I, and LC3II protein expression levels were evaluated by western blot. Results: We found that the survival rate of HepG2 cells was 47.42% when treated with Sorafenib (10 μmol/L) monotherapy, and 46.04% when treated with cisplatinum (10 mg/L) monotherapy. When Sorafenib(10 μmol/L) was combined with cisplatinum (10 mg/L), the cellular proliferation and survival rate was only 16.71% ( P <0.05). qPCR and western blot revealed that a combination of Sorafenib (10 μmol/L) and cisplatinum (10 mg/L) reduced the transcription and protein expression of autophagy-related AKT and mTOR but increased that of LC3 (P <0.05). Conclusion: Combining Sorafenib and cisplatinum can effectively induce cell autophagy and reduce cellular proliferation via the PI3K/AKT/mTOR signal pathway.
- انتشار مقاله: 06-01-1398
- نویسندگان: Yaoting Wang,Lei Wang
- مشاهده