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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: Ki-67 antigen,Oral epithelial dysplasia (OED),Oral squamous cell carcinomas (OSCC),Normal oral epithelium (NOE)
- چکیده:
- چکیده انگلیسی: Introduction: Abnormal cell proliferation appears to be a possible predictor of tumorigenesis, Ki-67 protein
expression is closely related to the cell proliferation and could be used as a biomarker for the growth in the most of
human tumors. The aim of the study: Investigating of Ki-67 expression in the pathological grades of oral epithelial
dysplasia and oral squamous cell carcinomas. Materials and Methods: The sample consisted of 30 formalin-fixed,
paraffin-embedded specimens of oral epithelial dysplasia (OED), 30 other of oral squamous cell carcinomas (OSCC), and
10 normal oral epithelium (NOE) were conventionally stained with hematoxylin and eosin and immunohistochemically
stained with Ki-67 monoclonal antibody. Results: Expression of Ki-67 was restricted to the basal layers in the normal
oral epithelium whereas Ki-67 positive cells in oral epithelial dysplasia (OED) were located in the basal, suprabasal
and spinous layers, Ki-67 expression was increased in high-risk cases. Ki-67 positive cells in well-differentiated
(OSCC) were located mainly in the periphery of the tumor nests, in moderately-differentiated (OSCC) were located
in both peripheral and part of a center of the tumor nests whereas it was diffused in most of the Poorly-differentiated
(OSCC). Statistical analysis indicated a significant difference between the expression in (OED) and (NOE), (OSCC)
and (NOE), and no differences between (OED) and (OSCC). Conclusion: This study has concluded that Ki-67 antigen
could be used as a marker for the histological grading of OED and OSCC, Expression of Ki 67 increased according to
the severity of oral epithelial dysplasia.- انتشار مقاله: 09-04-1397
- نویسندگان: Amer Takkem,Charif Barakat,Safa Zakarea,Khaled Zaid,Johnny Najmeh,Mahdi Ayoub,Mohammad Yaman Seirawan
- مشاهده
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: p53,Shisha,Malignancy,oral mucosa
- چکیده:
- چکیده انگلیسی: Introduction: Shisha (waterpipe) smoking is becoming a very prevalent form of tobacco consumption in the Middle
east and use is growing over the world. Smoking-related malignancies have a high genome-wide burden of mutations,
including examples in the gene encoding p53. Aims: To investigate alterations in p53 immunohistochemical expression
in the normal, pre-malignant, malignant oral mucosa in relation to Shisha smoking habits. Materials and Methods:
A total of 105 paraffin embedded tissue sections of OSCCs (52 smokers,53 non-smokers), 96 of premalignant lesions
(48 smokers,48 non-smokers) and 60 normal oral mucosa. Some 30 patients with a history of Shisha smoking daily
for more than 5 years were also investigated for mutant expression of p53. Tissue samples were considered positive
for p53 staining when any positive cells of epithelial origin could be detected. Results: The majority (74.3%) of oral
squamous cell carcinomas showed positive staining for p53 expression (83.1% and 65.5% with Shisha smokers and
non-smokers, respectively). In the 96 premalignant lesions, about 23% from non-smokers and 41.7% from smokers
showed p53 positivity. In normal epithelium, P53 positive cells were noted in 6.6% of non-smokers and 16.6% of
smokers. Positive correlations with Shisha smoking were evident for the following groups: WDOSCC, MDOSCC,
mild dysplasia G1, moderate dysplasia G2 and in normal mucosa using Student’s t- test, P valueThese results strongly suggest that p53 mutations are associated with Shisha smoking in OSCC, pre-malignant lesions
and normal mucosa of the oral cavity.- انتشار مقاله: 20-10-1396
- نویسندگان: Khaled Zaid,Elie Azar-Maalouf,Charif Barakat,Mansour Chantiri
- مشاهده
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: Ameloblastoma,Keratocystic odontogenic tumor,Amelogenin,Oncogenesis
- چکیده:
- چکیده انگلیسی: Amelogenin (AMEL), the major structural protein of the enamel organic matrix, constitutes more than 90% of the
enamel’s protein content, Aberrations of amelogenin are thought to be involved in the oncogenesis of odontogenic
epithelium. The expression of amelogenin is possibly an indicator of differentiation of epithelial cells in the odontogenic
tumors. Aim of the study: Investigating the expression of amelogenin in some odontogenic tumors, using an
anti-amelogenin polyclonal antibody, and then compare it with AMEL expression in tooth buds as control. Materials
and Methods: study sample consisted of 10 formalin-fixed, paraffin- embedded specimens of ameloblastoma,
10 Keratocystic odontogenic tumors, and 10 tooth buds were conventionally stained with hematoxylin-eosin and
immunohistochemically with AMEL polyclonal antibody. Results: All of the odontogenic tumors expressed AMEL
in the epithelial component, Intensity of expression in ameloblastoma and Keratocystic odontogenic tumor was lower,
compared with tooth buds, Statistical analysis indicated a significant differences between the tumors and tooth buds.
Conclusion: Amelogenin can be used as a marker for odontogenic epithelium, and the expression of amelogenin is
possibly an indicator of epithelial cells differentiation in the odontogenic tumors, and therefore in prediction of the
histological behavior of odontogenic tumors.- انتشار مقاله: 07-11-1396
- نویسندگان: Safa Zakaraia,Mamdouh Almohareb,Khaled Zaid,Mazen Doumani,Mohammad Yaman Seirawan
- مشاهده