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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: Advanced Research in Microbial Metabolites & Technology
- نوع مقاله: Journal Article
- کلمات کلیدی: Bio-controlling,Caspian Sea,Cystoviridae,Dental Plaque,Streptococcus salivarius,Tectiviridae
- چکیده:
- چکیده انگلیسی: The aggregation of oral Streptococci is resulted in the commence of enamel calcium degradation of the teeth and subsequently emergence of dental caries. The bacteriophages have considerable effects in controlling the populations of various bacteria. The isolation and characterization of specific lytic bacteriophages against oral streptococci were the main goals of this study. The BHI culture medium was used for oral streptococci enrichment from dental plaque. The MSA culture medium was applied to isolate oral streptococci from dental plaque samples. The membrane filtrate of Caspian Sea water was added to streptococcal isolate suspension and cultured using overlay method. The isolated bacterium on MSA according to macroscopic, microscopic and biochemical examinations identified as Streptococcus salivarius. The BLASTN of gtfK gene after DNA sequencing reconfirmed that our isolate was S. salivarius. The strain was named S. salivarius KBM-ISF-2 and its gtfK gene was deposited at GenBank, NCBI under accession number of KJ634208.1 Transmission electron microscopy revealed two specific lytic bacteriophages that attacked S. salivarius we isolated from dental plaques. The first lytic phage was a Tectivirus with icosahedral symmetry measuring 46.67 nm as thickness. The second phage was a Cystovirus with icosahedral symmetry and diameter of 85.56 nm. This is the first report of isolation and characterization of Tectiviruses and Cystoviruses from Caspian Sea and their specific preventing effects on oral streptococci. The lytic specific bacteriophages could be applied as a therapeutic measure for preventing the infectious diseases in human oral cavity.
- انتشار مقاله: 18-02-1398
- نویسندگان: Keivan Beheshti-Maal,Keivan Beheshti-Maal,Keivan Beheshti-Maal,Keivan Beheshti-Maal
- مشاهده
- جایگاه : پژوهشی
- مجله: Advanced Research in Microbial Metabolites & Technology
- نوع مقاله: Journal Article
- کلمات کلیدی: Vinegar,Ethanol,acetic acid,Acetobacter pasteurianus,Taguchi Statistical Design,Thermo-Tolerant Acetic Acid Bacteria
- چکیده:
- چکیده انگلیسی: Because of the high energy consumption for fermentor cooling, the isolation of thermo-tolerant Acetobacter strains for vinegar production has a high priority. The aims of this study were the isolation and identification of a high ethanol-thermo-tolerant Acetobacter spp. from grapes as well as the optimization of conditions for increasing the acetic acid production. The grape extract was cultured on Frateur and Carr media. The isolates were characterized against macroscopic, microscopic, and molecular traits. The resistance of the isolates against different concentrations of ethanol as well as high temperatures were measured. The best ethanol- thermo-tolerant isolate using ribotyping was identified as Acetobacter pasteurianus KBMNS-IAUF-2 and its 16s-rDNA sequence was deposited in GenBank, NCBI under the accession number MG547344. This strain was able to grow in Carr medium with 9% (v/v) ethanol after 48h incubation at 40°C. A Taguchi design with L9 analysis revealed that the highest yield of acetic acid production occurred at 34°C in the medium containing of 2% (v/v) ethanol and 2% (w/v) yeast extract. The temperature and yeast extract concentration with 76.70% and 6.29% were the most and least effective factors in acetic acid production, respectively. The acetic acid production yield after 24h under the optimized condition was measured as 97%. This thermo-ethanol-tolerant strain could be applied as an industrial starter for producing vinegar with grapevine flavor.
- انتشار مقاله: 04-03-1398
- نویسندگان: Keivan Beheshti-Maal,Noushin Shafiee
- مشاهده
- جایگاه : پژوهشی
- مجله: Advanced Research in Microbial Metabolites & Technology
- نوع مقاله: Journal Article
- کلمات کلیدی: Bio-controlling,Caspian Sea,Cystoviridae,Dental Plaque,Streptococcus salivarius,Tectiviridae
- چکیده:
- چکیده انگلیسی: The aggregation of oral Streptococci is resulted in the commence of enamel calcium degradation of the teeth and subsequently emergence of dental caries. The bacteriophages have considerable effects in controlling the populations of various bacteria. The isolation and characterization of specific lytic bacteriophages against oral streptococci were the main goals of this study. The BHI culture medium was used for oral streptococci enrichment from dental plaque. The MSA culture medium was applied to isolate oral streptococci from dental plaque samples. The membrane filtrate of Caspian Sea water was added to streptococcal isolate suspension and cultured using overlay method. The isolated bacterium on MSA according to macroscopic, microscopic and biochemical examinations identified as Streptococcus salivarius. The BLASTN of gtfK gene after DNA sequencing reconfirmed that our isolate was S. salivarius. The strain was named S. salivarius KBM-ISF-2 and its gtfK gene was deposited at GenBank, NCBI under accession number of KJ634208.1 Transmission electron microscopy revealed two specific lytic bacteriophages that attacked S. salivarius we isolated from dental plaques. The first lytic phage was a Tectivirus with icosahedral symmetry measuring 46.67 nm as thickness. The second phage was a Cystovirus with icosahedral symmetry and diameter of 85.56 nm. This is the first report of isolation and characterization of Tectiviruses and Cystoviruses from Caspian Sea and their specific preventing effects on oral streptococci. The lytic specific bacteriophages could be applied as a therapeutic measure for preventing the infectious diseases in human oral cavity.
- انتشار مقاله: 18-02-1398
- نویسندگان: Keivan Beheshti-Maal,Keivan Beheshti-Maal,Keivan Beheshti-Maal,Keivan Beheshti-Maal
- مشاهده