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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: Cholangiocarcinoma,proteomics,signaling pathway,Atractylodin,β-eudesmol
- چکیده:
- چکیده انگلیسی: Objective: The study aimed to identify potential cell signaling pathways and protein targets of actions of atractylodin and β-eudesmol in cholangiocarcinoma, the two active compounds isolated from Atracylodes lancea using proteomics approach. Method: The cholangiocarcinoma cell line, CL-6, was treated with each compound for 3 and 6 hours, and the proteins from both intra- and extracellular components were extracted. LC-MS/MS was applied following the separation of the extract proteins by SDS-PAGE and digestion with trypsin. Signaling pathways and protein expression were analyzed by MASCOT and STITCH software. Results: A total of 4,323 and 4,318 proteins were identified from intra- and extracellular components, respectively. Six and 4 intracellular proteins were linked with the signaling pathways (apoptosis, cell cycle control, and PI3K-AKT) of atractylodin and β-eudesmol, respectively. Four and 3 extracellular proteins were linked with the signaling pathways (NF-κB and PI3K-AKT) of atractylodin and β-eudesmol, respectively. Conclusion: In conclusion, a total of 17 proteins associated with four cell signaling pathways that could be potential molecular targets of anticholangiocarcinoma action of atractylodin and β-eudesmol were identified through the application of proteomics approach.
- انتشار مقاله: 23-04-1398
- نویسندگان: Kanawut Kotawong,Wanna Chaijaroenkul,Sittiruk Roytrakul,Narumon Phaonakrop,Kesara Na-Bangchang
- مشاهده
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: Cyclin D1,MAPK,Silica,B(a) P,HELF
- چکیده:
- چکیده انگلیسی: Objective: Silica and Benzo(a)pyrene are listed as carcinogens. This study aims to explore Cyclin D1, CDK4 and difference of cell cycle adjusted by MAPK signal transduction pathway in silica and B(a)P-induced malignant transformation of human embryonic lung fibroblasts. Methods: Activity of the subfamily (ERK, p38 and JNK) of mitogen-activated protein kinase (MAPK), cyclin D1 and CDK4 (cyclin dependent kinase) were evaluated using Human embryonic lung fibroblast (HELF) purchased from the cell room, basic research institute, Chinese Academy of Medical Sciences. The expression of cyclin D1 and CDK4 (cyclin dependent kinase) were measured in silica and B(a)P induced malignant using Western blot (WB) assay. Result: P-ERK and P-JNK expression increased significantly (P<0.01), while CDK4 and P-p38 expression decreased (P<0.01, P<0.05) in silica-induced malignant transformation cells compared with the control group. P-ERK, P-JNK and Cyclin D1 expression increased (P<0.01, P<0.01, P<0.05) in B(a)P-induced group compared with the control group. P-ERK and P-JNK expression decreased (P<0.01), while P-p38, Cyclin D1 and CDK4 expression increased (P<0.05, P<0.05, P<0.01) in B(a)P-induced group compared with the silica-induced group. Conclusion: MAPK and cyclin D1/CDK4 activation expressed differently in human embryo lung fibroblasts malignant transformation induced by silica and benzopyrene.
- انتشار مقاله: 08-09-1396
- نویسندگان: Huan Wang,Shuyu Xiao,Yali Tang,Ke Han,Zheng Zhang,Yulan Jin,Fuhai Shen
- مشاهده
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: Cholangiocarcinoma,Systematic review,statistics,Statistical misuse
- چکیده:
- چکیده انگلیسی: Objective: The aim of the study was to perform a systematic review of research articles related to cholangiocarcinoma (CCA), the bile duct cancer in Southeast Asian (SEA) countries published during 2010-2015 including analysis of inappropriate use/misuse of statistics. Methods: Research articles were retrieved from the PubMed database using different ‘keywords’ for seven research disciplines/categories in biomedical sciences (medicine/physiology, epidemiology, immunology, pharmacology and toxicology, diagnosis/diagnostics, drug resistance, and biochemistry). Results: A total of 353 articles were finally included in the analysis based on the pre-defined eligibility criteria. Most were articles of which the studies were conducted in Thailand (335 articles, 94.90%). Disease diagnosis/diagnostics (n=266, 75.35%), biochemistry (n =223, 63.17%), and pharmacology and toxicology (n =218, 61.76%) were the three main research disciplines/categories for CAA conducted in SEA countries during 2010-2015. Thailand was the country which most published CCA-related research articles in all disciplines/categories. Drug resistance was the research category that most applied both descriptive and inferential statistics (100%). The student’s t-test was the most applied test (35.13%). Inappropriate use/misuse of statistics in all types was highest in diagnosis/diagnostics (73.59%) and pharmacology and toxicology (73.06%) research disciplines/categories and was lowest in medicine/pathophysiology (0.26%). Inappropriate use/misuse in almost all types (seven types) was found in the diagnosis/diagnostics category. Conclusion: Results of the systematic analysis of CCA-related research articles published from the ten SEA countries during 2010-2015 reveal high rates of inappropriate use/misuse of statistics. The readers should be aware of the reliability of the articles and the possibility of wrong interpretation and conclusion of these articles.
- انتشار مقاله: 16-01-1398
- نویسندگان: Panida Kongjam,Lakshmanan Jeyaseelan,Wanna Chaijaroenkul,Kesara Na-Bangchang
- مشاهده
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: response,Vitamin D pathway polymorphisms,pegylated-interferon,HBeAg-negative,Hepatitis B virus infection
- چکیده:
- چکیده انگلیسی: Background: Vitamin D deficiency is related to poor clinical outcomes in patients with chronic hepatitis B virus
(HBV) infection. Methods: We aimed to investigate the association between the genetic variants in the vitamin D
metabolic pathway and the response to pegylated interferon (Peg-IFN) therapy in patients with HBeAg-negative
chronic HBV infection. One hundred seven patients treated with Peg-IFN for 48 weeks were selected from 13 specialty
hospitals. Eight genotypes of vitamin D cascade genes, including CYP27B1 (rs10877012), DHCR7 (rs12785878),
CYP2R1 (rs2060793, rs12794714) and GC (rs4588, rs7041, rs222020, rs2282679), were found. Results: Eighty-two
patients (83.7%) were infected with HBV genotype C. Eight patients had compensated liver cirrhosis (8.7%). At 24
weeks after treatment discontinuation, 41 patients (42.3%) achieved sustained treatment response, 53 (55.2%) obtained
HBV DNA<2,000 IU/ml, 6 (5.6%) gained HBsAg seroclearance, 2 (1.9%) had HBsAg seroconversion and 69 (64.5%)
exhibited alanine aminotransferase (ALT) normalization. Multivariate analysis revealed that baseline HBsAg level (OR
=0.06, 95% CI: 0.08-0.49, p=0.008) and the GC rs222020 TT genotype (OR=17.72, 95% CI: 1.07-294.38, p=0.04)
independently predicted sustained HBsAg seroclearance. In addition, this genotype was a predictor for normalization of
ALT (OR=4.61, 95%CI: 1.59-13.40, p=0.005) after therapy. The HBsAg levels at baseline and during and post-treatment
tended to be reduced with the GC rs222020 TT compared with the non-TT genotypes. The other studied polymorphisms
were not associated with treatment response. Conclusions: The GC rs222020 TT genotype, which is a variant in the
vitamin D-binding protein gene, could identify HBeAg-negative patients who have a high probability to achieve HBsAg
clearance and ALT normalization after treatment with Peg-IFN.- انتشار مقاله: 09-10-1397
- نویسندگان: Kessarin Thanapirom,Sirinporn Suksawatamnuay,Wattana Sukeepaisarnjaroen,Sombat Treeprasertsuk,Tawesak Tanwandee,Phunchai Charatcharoenwitthaya,Satawat Thongsawat,Apinya Leerapun,Teerha Piratvisuth,Rattana Boonsirichan,Chalermrat Bunchorntavakul,Chaowalit Pattanasirigool,Bubpha Pornthisarn,Supot Tuntipanichteerakul,Ekawee Sripariwuth,Woramon Jeamsripong,Theeranan Sanpajit,Yong Poovorawan,Piyawat Komolmit
- مشاهده