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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: Iranian Journal of Veterinary Research
- نوع مقاله: Journal Article
- کلمات کلیدی: PCR,Goats,electrophoresis,Babesia ovis,Serum protein
- چکیده: مطالعه حاضر جهت تعیین الگوی الکتروفورتیک پروتئینهای سرم در بزهای آلوده به بابزیا اویس انجام شد. محاسبه مقدار پروتئین کل سرم و الکتروفورز پروتئینهای سرم 15 بز آلوده و سالم انجام شد. آلودگی به بابزیا اویس با استفاده از آزمایش میکروسکوپی مشخص گردید. تایید تشخیص آلودگی با استفاده از واکنش زنجیرهای پلیمراز و یک جفت پرایمر اختصاصی بابزیا اویس انجام شد. مقدار متوسط پروتئین کل و قطعات آلفا و گاما گلوبولین در گروه آلوده نسبت به گروه سالم به طور معنیداری بالاتر بود (P<0.05).
- چکیده انگلیسی: This study was conducted to determine the electrophoretic pattern of serum protein in goats infected with
Babesia ovis. Serum total protein was calculated and serum electrophoresis from 15 goats naturally infected
with B. ovis as well as same number of healthy goats was performed. Microscopic examination of Giemsastained peripheral blood smears revealed B. ovis infection. The parasitological diagnosis was confirmed using polymerase chain reaction (PCR) assay by using a pair of B. ovis-specific primers. Mean values total protein and α and γ globulin fractions in infected goats were significantly higher (P<0.05) than those observed in the healthy group.- انتشار مقاله: 10-11-1390
- نویسندگان: B. Esmaeilnejad,M. Tavassoli,S. Asri-Rezaei,B. Dalir-Naghadeh,K. Mardani,F. Farhaghpajouh,S. M. Abtahi
- مشاهده
- جایگاه : پژوهشی
- مجله: Archives of Razi Institute
- نوع مقاله: Journal Article
- کلمات کلیدی: Drug resistance,bovine,mastitis,Shige toxin-producing Escherichia coli (STEC),virulence
- چکیده:
- چکیده انگلیسی: Bovine subclinical mastitis is regarded as a devastating disease due to the economic costs imposed on dairy husbandry. Moreover, it is a hazard in the public sector in the cases of zoonotic bacteria because of the potential role of unpasteurized milk and dairy products to propagate the infectious agent to the human food chain. The present study aimed to evaluate the frequency, virulence content, and antimicrobial resistance profile of Shiga toxin-producing Escherichia coli (STEC) strains isolated from bovine subclinical mastitis in Kurdistan Province, West of Iran. A total of 400 bovine subclinical mastitis milk samples recognized in the California Mastitis Test were collected aseptically and analyzed for the presence of E. coli phenotypically and molecularly. The isolates were genotypically screened for stx1, stx2, and eae genes. Furthermore, O157:H7 STEC strain was searched among the isolates in a duplex polymerase chain reaction. The antimicrobial resistance scheme of the isolates was determined using the agar disk diffusion method. In general, 173 (43.25%) E. coli isolates were detected among which 39 (22.54%) isolates were STEC. The frequency of STEC virulence genotypes was stx2 (25 isolates, 64.10%), stx2+eae (6 isolates, 15.38%), stx1+stx2 (6 isolates, 15.38%), and stx1+stx2+eae (2 isolates, 5.12%). In addition, three O157: H7 strains were identified with the genetic content of stx1+stx2+eae (2 isolates) and stx1+stx2 (1 isolate). The most prevalent antimicrobial resistance was observed against streptomycin, tetracycline, and ampicillin. Gentamycin, amoxicillin-clavulanic acid, and trimethoprim-sulfadiazine were the most effective antibiotics against O157 strains, whereas gentamycin, ciprofloxacin, and nitrofurantoin were effective against non-O157 strains. The results revealed the significant role of STEC in bovine subclinical mastitis in the studied region. In addition, the distribution of O157:H7 strain and high prevalence of multidrug resistance among the isolates is a matter of concern. Therefore, there is a potential threat of human infection following the consumption of contaminated milk with STEC in Kurdistan Province, Iran.
- انتشار مقاله: 14-09-1397
- نویسندگان: E. Ahmadi,K. Mardani,A. Amiri
- مشاهده
- جایگاه : پژوهشی
- مجله: Archives of Razi Institute
- نوع مقاله: Journal Article
- کلمات کلیدی: Iran,Sheep,Neospora caninum,Toxoplasma gondii,Goats
- چکیده:
- چکیده انگلیسی: Toxoplasma gondii and Neospora caninum are Apicomplexan intracellular protozoa with global distribution. Small ruminants play an important role as intermediate hosts for N. caninum and T. gondii, parasites of great public health concern. The main goal of the current survey was to evaluate N. caninum and T. gondii infection rate in sheep and goats of Khuzestan Province, southwest of Iran, using enzyme-linked immunosorbent assay (ELISA). In this cross-sectional study during February-April 2016, whole blood samples were taken randomly from 735 animals from 37 herds. The animals were reared under the traditional husbandry system in different parts of the province. Among 550 sheep and 185 goats, 37 (6.8%) sheep and 20 (10.8%) goats were seropositive for N. caninum and 59 (10.8%) sheep and 37 (20%) goats were seropositive for T. gondii. The incidence rates of mixed infection with N. caninum and T. gondii were 3.2% and 5.4% in sheep and goats, respectively. Seroprevalence rate of N. caninum was significantly higher in goats at <1 years of age. There was a significant association between the number of sheep infected with T. gondii and abortion (18.2%). Also, a significant correlation was detected between seroprevalence of N. caninum and T. gondii and mixed infection in goats with a history of abortion. This is the first report of IgG antibody production against N. caninumand T. gondii co-infection in small ruminants in Iran. Our findings indicated that neosporosis and toxoplasmosis may be responsible for abortion in small ruminants in this region. Therefore, further investigations are needed to improve sanitary strategies in animals’ husbandry and launching control programs.
- انتشار مقاله: 23-12-1395
- نویسندگان: J. Gharekhani,M. Yakhchali,B. Esmaeilnejad,K. Mardani,G. Majidi,A. Sohrabei,R. Berahmat,M. Hazhir Alaei
- مشاهده