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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: Breast cancer,PCR-RFLP,XRCC4 SNP,Filipinos
- چکیده:
- چکیده انگلیسی: Background: The identification of cancer-associated single nucleotide polymorphisms (SNP) and mutation
genes is a promising approach in recognizing individuals who are at risk of developing cancer. Hence, this study
was conducted to determine the association between XRCC4 c.1394G>T SNP and breast cancer development among
Filipinos. Methods: Genotyping for XRCC4 c.1394G>T SNP was performed on breast cancer patients (n=103) and
their age- and sex- matched clinically healthy controls (n=103) by polymerase chain reaction – restriction fragment
length polymorphism. Results: Significant difference in genotype (p=0.007) and allele (p=0.003) frequencies in XRCC4
c.1394G>T was observed between the breast cancer cases and controls. Carriers of the XRCC4 c.1394 G>T genotype
were observed to have significantly higher risk of developing breast cancer compared to individuals with T/T genotype
(OR=2.67, 95% CI: 1.36 – 5.25). XRCC4 c.1394G>T combined with passive smoking may also significantly increase
risk of breast cancer (OR=14.73; 95% CI= 9.88-18.86). Conclusion: XRCC4 c. 1394G>T may be associated with
breast cancer development among Filipinos.- انتشار مقاله: 12-05-1397
- نویسندگان: Julius Adrie Garcia,Noel Angelo Kalacas,Teresa Sy Ortin,Maria Cristina Ramos,Pia Marie Albano
- مشاهده
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: Breast cancer,Genetic polymorphism,GSTM1,GSTT1
- چکیده:
- چکیده انگلیسی: Background: The association of genetic polymorphisms with cancer development has been shown to be race- and
tumor site-specific. Thus, this study aimed to determine whether polymorphisms in the GSTM1 and GSTT1 genes
are associated with breast cancer among selected Filipinos. Methods: A total of 136 histologically confirmed breast
cancer cases were age- and sex-matched with 136 clinically healthy controls. Genomic DNA extracted from blood
samples of participants were screened for GSTM1 and GSTT1 genetic polymorphisms by multiplex PCR. Results:
The frequency of null genotypes among the cases (GSTM1: n=78; 57.4%; GSTT1: n=61; 44.9%) was not significantly
different (p>0.05) from the controls (GSTM1: n=93; 68.4%; GSTT1: n=59; 43.4%). It was also demonstrated that risk
for breast cancer was increased in passive smokers carrying the GSTM1 null (OR=2.56; 95% CI=1.38-4.75) or GSTT1
positive (OR=2.00; 95% CI=1.05-3.83) genotypes. Moreover, risk was decreased in alcohol users carrying the GSTT1
null (OR=0.39; 95% CI=0.16-0.97) genotype. Conclusion: This study suggests that variants of GSTM1 and GSTT1
may not be risk factors for breast cancer development among Filipinos. However, the risk may be increased when these
genotypes were combined with lifestyle or environmental factors.- انتشار مقاله: 14-04-1397
- نویسندگان: Noel Angelo Kalacas,Julius Adrie Garcia,Teresa Sy Ortin,Alfredo Valdez Jr,Allan Fellizar,Maria Cristina Ramos,Pia Marie Albano
- مشاهده