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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: Prostate cancer,progesterone receptor (PR),benign prostatic hyperplasia,transcription and growth factors,AKT/mTOR signaling pathway components
- چکیده:
- چکیده انگلیسی: Background: Progesterone receptor (PR) is a critical regulator in reproductive tissues that controls a variety of cellular processes. The objective of the study was to study the PR expression in patients with benign prostatic hyperplasia and prostate cancers in connection with the transcription, growth factors, AR, ERα, ERβ, and components of the AKT/mTOR signaling pathway expression. Materials and methods: Ninety-seven patients with prostate pathology were enrolled in the study. Forty-two patients had benign prostatic hyperplasia (BH). Fifty-five patients had locally advanced prostate cancer (PCa). The PSA level and the amount of testosterone in the serum were measured using an ELISA assay. The expression level of NF-κB p65, NF-κB p50, HIF-1, HIF-2, growth factor VEGF, VEGFR2, CAIX, as well as AR, ERα, ERβ, PR, Brn-3α, TRIM16 were quantified by RT-PCR. The protein level of Brn-3α, TRIM16 was detected by Western Blotting. Results: Growth in PR expression was observed in PCa tissues compared to BH ones without changes in the clinical and pathological features of the patients. An increase in PR expression was detected in patients with PCa compared to BH. Its mRNA level depended on the expression of AR, Brn-3α, and TRIM16, components of the AKT/mTOR signaling pathway, transcription, and growth factors. An increase in the TRIM16 expression in the PCa tissues was noted in the case of a low PR level. We revealed the growth in PR expression was accompanied by the suppression of the signaling cascade activity, AR, Brn-3α mRNA level, and the enhanced PTEN expression in PCa tissues. The increase in PR expression in PCa led to a decrease in the level of mRNA of NF-κB, HIF-1, VEGF, and VEGFR2. Conclusion: In general, the data indicated the significance of the PR expression in the development of the prostate pathology that affected the cross-talk between the steroid hormone reception and signal transduction.
- انتشار مقاله: 02-05-1398
- نویسندگان: Liudmila V Spirina,Irina V Kovaleva,Evgeny A Usynin,Alexey K Goorbunov,Irina V Kondakova
- مشاهده
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: Colorectal cancer,exosomes,20S proteasome,ADAM10,ADAM17
- چکیده:
- چکیده انگلیسی: Purpose: Exosomal proteases are important in regulation of molecular signaling from growth factor receptors and
adhesion molecules and also the regulation of cell motility and protein folding. The aim of this study was to evaluate
the level of ADAM10, ADAM17 and 20S proteasomes in exosomes isolated from colorectal cancer patients (CRCPs)
in relation with clinical and histopathological parameters. Methods: Blood plasma exosomes of 60 CRCPs at stage
T2-4N0-2M0-1 and 10 control subjects (CSs) with colorectal polyps were isolated using ultrafiltration in combination
with ultracentrifugation. The level of tetraspanin-associated (ADAM20 and ADAM17) and tetraspanin-non-associated
(20S proteasome) proteases were evaluated by flow cytometry and western blot analysis. Results: The ADAM10-/
ADAM17- population predominated in plasma exosomes of CRCPs and the level of ADAM10+ exosomes was
significantly higher in exosomes of CSs compared with CRCPs. No difference was found between subpopulations
of ADAM10/ADAM17 exosomes and level of exosomal 20S proteasomes in terms of sex, age and tumor grade.
Simultaneous decrease of ADAM10+/ADAM17-subpopulation of exosomes and level of exosomal 20S proteasomes in
patients with metastatic CRC was observed compared with patients with non-metastatic CRC. The level of ADAM17+
exosomes significantly reduced in exosomes of CRCPs with metabolic syndrome compared to CRCPs without
metabolic syndrome( 3.97±0.71 (%) vs. 13.04±1.34 (%), respectively (p<0.05). A decrease in the 20S proteasomes
level in plasma exosomes was revealed in CRCPs with metabolic syndrome compared with CRCPs without metabolic
disorders ( 1.90±0.25 (r.u.) vs. 2.92±0.42 (r.u.) respectively( (p<0.05). Conclusion: According to findings of this study,
it seems that exosomal proteases can be promising molecular predictors of hematogenous metastasis in patients with
non-metastatic CRC. Further studies on subpopulation composition of exosomes CRCPs are need for elucidating the
role of tetraspanin-associated and tetraspanin-non-associated exosomal proteases in CRC development and progression.- انتشار مقاله: 17-05-1397
- نویسندگان: Elena A Tugutova,Svetlana N Tamkovich,Marina R Patysheva,Sergey G Afanas’ev,Anastasia A Tsydenova,Alina E Grigor’eva,Elena S Kolegova,Irina V Kondakova,Natalia V Yunusova
- مشاهده
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: Breast cancer,Ovarian Cancer,exosomes,ADAM-10,20S proteasome
- چکیده:
- چکیده انگلیسی: Background: As is known, exosomes play an important role in promoting progression of cancers by increasing
its invasive potential. The aim of this study was to evaluate the levels of tetraspanine-associated (ADAM-10) and
tetraspanine-nonassociated proteases (20S proteasomes) in exosomes from culture medium, plasma exosomes of
patients with breast tumors and plasma and ascites of ovarian tumor patients. Methods: MCF-7 and SVO-3 culture
mediums and blood samples from healthy females (n = 30, HFs), patients with diffuse dyshormonal dysplasia of the
breast (n=28, BBTPs), breast cancer patients (n=32, BCPs), borderline ovarian tumor patients (n=20, BOTPs) and
blood and ascites samples ovarian cancer patients (n=35, OCPs) were included in the study. Exosomes from plasma,
ascites and culture mediums were isolated and characterized in according to Extracellular Vesicles Society. The
expression levels of 20S proteasome and ADAM-10 in exosomes were determined using flow cytometry and western
blot analysis, correspondingly. Results: The subpopulation composition of the exosomes from MCF-7 culture medium
and from blood plasma of HFs and breast diseases patients is similar, however CD9/CD24 subpopulation significantly
increased at cell supernatant. The similar results was obtained for exosomes from SVO-3 medium and blood plasma
and ascites of ovary tumor patients, but CD9/CD24 subpopulation significantly decreased at cells and illness samples,
however CD63/CD24 exosomes increased significantly from cell supernatant. 20S proteasome level is significantly
increased in exosomes from MCF-7 and SVO-3 culture medium, breast tumor patients and OCPs plasma in comparison
to HUVEC culture medium and HFs plasma samples. At CD9-positive exosomes from BCPs plasma and MCF-7 was
reveal a high expression of ADAM-10 and low expression is from BBDPs plasma and ovarian tumor patients plasma/
ascites samples. Exosomes from ascites OCP had high expression of ADAM-10 in the CD24-positive subpopulation.
Conclusion: Breast and ovarian cancer development is connected with functioning of immune proteasome forms in
plasma and ascites exosomes, while increased ADAM10 expression at CD9-positive exosome was associated with
breast cancer and at CD24-positive subpopulation – with ovarian cancer. Obtained data confirm role of exosomal
proteases in tumor progression.- انتشار مقاله: 31-05-1397
- نویسندگان: Svetlana N Tamkovich,Natalia V Yunusova,Elena Tugutova,Anton K Somov,Ksenia V Proskura,Larisa A Kolomiets,Marina N Stakheyeva,Alina E Grigor’eva,Pavel P Laktionov,Irina V Kondakova
- مشاهده