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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: Iranian Journal of Medical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: Ovarian Neoplasms,Ki-67 antigen,Endothelin-1,Receptor,Endothelin A,Heparanase
- چکیده:
- چکیده انگلیسی: Background: Heparanase and endothelin-1/endothelin A receptor (ET-1/ETAR) expressions increase in cancer. This condition enhances tumor progression and correlates with poor survival. Limited data are documented regarding the role of heparanase and ET-1/ETAR in epithelial ovarian cancer (EOC). We sought to characterize the correlation between heparanase and ET-1/ETAR in EOC.Methods: Thirty patients with benign and malignant ovarian neoplasms were recruited in this study. Neoplasm subtypes were diagnosed by pathologists. RNA extraction was done in fresh frozen neoplasms while immunohistochemical (IHC) staining was done on ETAR, heparanase, and proliferation (Ki-67 antigen) in paraffin sections. Reverse transcriptase PCR was done to quantify the expression of preproET-1 (ppET-1), ETAR, and heparanase. ETAR and heparanase histoscores were done based on IHC staining. The Independent Samples t Test, ANOVA, and correlations were used for statistical analysis. Results: Heparanase and ETAR histoscores, ppET-1 and ETAR mRNA levels, and Ki-67 were significantly higher in the group with EOC than in the benign or borderline group, regardless of the histopathological types. The heparanase histoscore correlated with the ETAR histoscore (r=0.484, P=0.007) and the ETAR mRNA level (r=0.551, P=0.003). The level of ppET-1 mRNA correlated with both ETAR mRNA level and ETAR histoscore (r=0.603, P=0.001 and r=0.455, P=028, respectively). The ovarian neoplasms with high ppET-1 mRNA levels also tended to have high heparanase mRNA levels; however, the correlation was weak (r=0.354, P=0.07). Ki-67 correlated with the heparanase and ETAR histoscores (r=0.381, P=0.038 and r=0.477, P=0.008, respectively).Conclusion: Heparanase and ETAR were upregulated in EOC, and the correlation between heparanase and ETAR expressions was also elucidated in the current study.
- انتشار مقاله: 08-09-1395
- نویسندگان: Nungki Anggorowati,Ahmad Ghozali,Irianiwati Widodo,Dwi Cahyani Ratna Sari,Muhammad Mansyur Romi,Nur Arfian
- مشاهده
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: Breast cancer,Survival,VEGF,Ki-67,Omega-3
- چکیده:
- چکیده انگلیسی: Background: Omega-3 is a polyunsaturated fatty acid with an ability to regulate cell proliferation and apoptosis
through interaction with inflammatory mediators. The potential additional beneficial effects of Omega-3 on chemotherapy
patients with breast cancer is not yet completely revealed. Methods: A double-blind randomized control trial (RCT)
involving a total of 48 locally advanced breast cancer patients was conducted. Ki-67 and VEGF expressions, as well as
overall survival of patients receiving neoadjuvant cyclophosphamide-doxorubicin-5’fluorouracyl (CAF) chemotherapy
plus Omega-3 (intervention group) or placebo (control group), were compared. Kaplan-Meier curve and Cox-regression
tests were used to assess conditional disease-free survival (DFS) and overall survival (OS) between the two groups.
Results: Decreased Ki-67 expression was observed in the intervention group compared to control (42.4±4.8 versus
39.2±5.3; T-test p=0.032). Decreased Ki-67 expression was observed in intervention compared to control group (42.4±4.8
versus 39.2±5.3; T-test p=0.032). Decreased VEGF expression was also seen in the intervention group compared to
control (32.7±5.2 versus 29.5±5.4; T-test p=0.041). VEGF expression positively correlated with Ki-67 expression
(Spearman’s test p<0.001, R2=0.541). Overall survival in the intervention group was significantly longer in comparison
to the control group (mean survival: 30.9 ± 3.71 versus 25.9 ± 3.6 weeks, Mantel-Cox test p=0.048; HR=0.411, 95%CI:
0.201-0.840). Disease-free survival was significantly longer in the intervention group compared to the control group
(mean survival: 28.5 ± 3.3 versus 23.7 ± 3.6, respectively; Mantel-Cox test p=0.044, HR= 0.439, 95%CI: 0.222-0.869).
Conclusion: Omega-3 fatty acid supplementation improved overall survival and progression-free survival of locally
advanced breast cancer treated with CAF neoadjuvant chemotherapy and mastectomy.- انتشار مقاله: 05-08-1397
- نویسندگان: Darwito Darwito,Edi Dharmana,Ignatius Riwanto,Selamat Budijitno,Suwardjo Suwardjo,Joko Purnomo,Irianiwati Widodo,Ahmad Ghozali,Teguh Aryandono,Sumadi Lukman Anwar
- مشاهده
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: Survival,Breast carcinoma,Luminal and non-luminal subtype,lymphangiogenesis,VEGF-C
- چکیده:
- چکیده انگلیسی: Background: Breast carcinomas (BCs) are sub-classified according to the molecular characteristics into luminal and
non-luminal subtypes that clinically show different biological behavior, treatment and prognosis. BCs spread primarily
through lymphatic vessels using cascade processes of lymphagiogenesis in which VEGF-C plays an important role during
lymph node metastasis. Prognostic value of VEGF-C in luminal and non-luminal BC is still unclear and has not been
studied thoroughly to clarify and define prognosis and therapeutic monitoring. Aim: To define the prognostic value of
lymphangiogenesis on survival rates of luminal and non-luminal subtypes BC. Materials and Methods: This study
applied prospective cohort design, using 130 patients of invasive duct carcinoma of the breast, stage I-IIIA, from
Sardjito General Hospital, Indonesia and subsequent longitudinal follow-up. Immunohistochemical staining was
carried out using anti-ER, -PR, -Her-2, VEGF-C, VEGFR-3 and D2-40 antibodies. The related clinicopathologic
characteristics of BC patients and lymphangiogenesis determinants, including VEGF-C expression, were statistically
analyzed. Results: In non-luminal BC subtypes, VEGF-C expression (HR=0.04; 95% CI=0.01-0.41), lymph node
metastasis (HR=0.14; 95% CI=0.04-0.55) and stage (HR=0.30; 95% CI= 0.02-0.76) were determined as independent
prognostic factors on survival rates. However, the lymphangiogenesis determinants were not associated with the survival
rates of luminal BC subtypes. Conclusion: This study suggested that lymphangiogenesis affects survival rates of
non-Luminal subtype rather than the luminal subtypes of BC.- انتشار مقاله: 12-06-1396
- نویسندگان: Irianiwati Widodo,Ery Kus Dwianingsih,Totok Utoro,Sumadi Lukman Anwar,Teguh Aryandono,Soeripto .
- مشاهده
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: molecular subtypes,Breast carcinoma,histological grade,lymph node metastases
- چکیده:
- چکیده انگلیسی: Objective: Breast carcinoma is a heterogeneous disease which is rich in diversity. Molecular subtypes of breast
cancer, histological grade and lymph node metastases are strong prognostic and predictive factors. In Indonesia,
only a limited number of studies have investigated the correlation between molecular subtypes with histological
grade and lymph node metastases. Methods: We analyzed 247 invasive breast carcinoma cases from the Anatomic
Pathology Installation of Dr. Sardjito General Hospital Yogyakarta between 2012-2015. The slides were stained for
estrogen receptors (ER), progesterone receptors (PR), HER2, Ki-67 and CK5/6 for classification into breast cancer
subtypes (BCS). Histological grade using the Nottingham system and lymph node status were obtained from anatomic
pathology records. The association between histological grade and lymph node status with BCS was examined with
Chi-square tests. Results: The immunohistochemical features of 247 cases of women with invasive breast carcinoma
were examined. There were 102 (41.3%) patients with Luminal A, 34 (13.8%) patients with Luminal B, 48 (19.4%)
patients with HER2-positive, and 63 (25.5%) patients with triple negative breast cancer (TNBC). There were 148
(59.9%) patients with negative lymph node status and 99 (40.1%) with positive status. Among 63 TNBC cases, 37
(58.7%) patients were positive for CK5/6 staining (basal-like). Statistically, there were significant differences between
histological grade and subtypes (p=0.013). However, no significant differences were found for lymph node metastases
(p=0.540). Conclusion: Among subtypes, Luminal A has the highest frequency, followed by TNBC, HER2-positive
and Luminal B. Histological grade was associated with molecular subtypes of breast carcinoma in Yogyakarta. Grade
I was associated with Luminal A, while Grade III was associated with Luminal B, HER2 and TNBC subtypes.- انتشار مقاله: 18-06-1396
- نویسندگان: Yunita Setyawati,Yeni Rahmawati,Irianiwati Widodo,Ahmad Ghozali,Dewajani Purnomosari
- مشاهده
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: age,molecular subtypes,Breast carcinoma,tumor size
- چکیده:
- چکیده انگلیسی:
Objective: Breast carcinoma (BC) is a heterogeneous disease that exhibits variation in biological behaviour, prognosis and response to therapy. Molecular classification is generally into Luminal A, Luminal B, HER2+ and triple negative/basal-like, depending on receptor characteristics. Clinical factors that determined the BC prognosis are age and tumor size. Since information on molecular subtypes of Indonesian BCs is limited, the present study was conducted, with attention to subtypes in relation to age and tumor size. Methods: A retrospective cross-sectional study of 247 paraffin-embedded samples of invasive BC from Dr. Sardjito General Hospital Yogyakarta in the year 2012- 2015 was performed. Immunohistochemical staining using anti- ER, PR, HER2, Ki-67 and CK 5/6 antibodies was applied to classify molecular subtypes. Associations with age and tumor size were analyzed using the Chi Square Test. Results: The Luminal A was the most common subtype of Indonesian BC (41.3%), followed by triple negative (25.5%), HER2 (19.4%) and luminal B (13.8%). Among the triple negative lesions, the basal-like subtype was more frequent than the non basal-like (58.8 % vs 41.2%). Luminal B accounted for the highest percentage of younger age cases (< 40 years old) while HER2+ was most common in older age (> 50 years old) patients. Triple negative/basal-like were commonly large in size. Age (p = 0.080) and tumor size (p = 0.462) were not significantly associated with molecular subtypes of BC. Conclusion: The most common molecular subtype of Indonesian BC is luminal A, followed by triple-negative, HER2+ and luminal B. The majority of triple-negative lesions are basal-like. There are no association between age and tumor size with molecular subtypes of Indonesian BCs.- انتشار مقاله: 18-06-1396
- نویسندگان: Yeni Rahmawati,Yunita Setyawati,Irianiwati Widodo,Ahmad Ghozali,Dewajani Purnomosari
- مشاهده