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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: Archives of Bone and Joint Surgery
- نوع مقاله: Journal Article
- کلمات کلیدی: Rat,Valproic acid,Bcl-2,Myelin,Growth cone-associated protein 43,Sciatic nerve index
- چکیده:
- چکیده انگلیسی: Background:
Valproic acid (VPA) is used to be an effective anti-epileptic drug and mood stabilizer. It has recently
been demonstrated that VPA could promote neurite outgrowth, activate the extracellular signal regulated kinase pathway,
and increases bcl-2 and growth cone-associated protein 43 levels in spinal cord. In the present research we
demonstrate the effect of VPA on peripheral nerve regeneration and recovery of motor function following sciatic nerve
transaction in rats.
Methods:
The rats in VPA group and control group were administered with valproic acid (300mg/kg) and sodium
chloride respectively after operation. Each animal was observed sciatic nerve index (SFI) at 2-week intervals and
studied electrophysiology at 4-week intervals for 12 weeks. Histological and morphometrical analyses were performed
12 weeks after operation. Using the digital image-analysis system, thickness of the myelin sheath was measured, and
total numbers of regenerated axons were counted.
Results:
There was a significant difference in SFI, electrophysiological index (motor-nerve conduct velocity), and
morphometrical results (regenerated axon number and thickness of myelin sheath) in nerve regeneration between the
VPA group and controls (
P<0.05).
Conclusions:
The results demonstrated that VPA is able to enhance sciatic nerve regeneration in rats, suggesting
the potential clinical application of VPA for the treatment of peripheral nerve injury in humans.- انتشار مقاله: 12-10-1392
- نویسندگان: Ting Rao,Fei Wu,Danmou Xing,Zhengren Peng,Dong Ren,Wei Feng,Yan Chen,Zhiming Zhao,Huan Wang,Junweng Wang,Wusheng Kan,Qingsong Zhang
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Basic Medical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: Rheumatoid arthritis,Invasion,Fibroblast-like synoviocyte,MicroRNA-21,Smads,Transforming growth factor-beta
- چکیده:
- چکیده انگلیسی: Objective(s): MicroRNA-21 (miR21) is aberrantly elevated in rheumatoid arthritis (RA) patients, the significance of this microRNA in RA pathogenesis and treatment, however, has not been investigated. In this study, by using RA-derived fibroblast-like synoviocyte (FLS) cells as a model, we investigated the effect and corresponding mechanism of miR21 inhibition on FLSs invasion.
Materials and Methods:miR21 expression in synovial tissue and FLSs in RA patients and non-RA controls were determined by stem-loop RT-PCR. The effect of miR21 on FLSs viability and invasiveness were evaluated using miR21 inhibition. Cell viability was evaluated by MTT assay and the expression of genes at mRNA and protein levels was determined by RT-PCR and Western blot, respectively.
Results: Our results showed that miR21 expression was highly increased in synovial tissue and FLSs in RA patients. Also, we reported that miR21 inhibitor treatment could significantly suppress the invasiveness of FLSs without affecting cell viability. The decreased FLSs invasion by miR21 inhibition was associated with down-regulated expression of matrix metalloproteinase (MMP)-1, MMP3, and MMP13. Further analysis revealed that miR21 inhibition could suppress the expression of TGFβ1 and Smad4, but promote that of Smad7. Moreover, suppression of FLS invasion and MMPs expression by miR21 treatment could be counteracted by additional TGFβ1 treatment.
Conclusion:Our results indicated that miR21 inhibition can down-regulate the expression of MMP1, MMP3, and MMP13 and consequently suppress the invasiveness of FLS, which is achieved through TGFβ1/Smad4/7 signaling pathway. The findings of this study could offer a novel approach for RA treatment.- انتشار مقاله: 17-05-1395
- نویسندگان: Gaoxin Xiong,Zhang Huang,Hua Jiang,Zhengjun Pan,Jie Xie,Shuangli Wang
- مشاهده
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: Cyclin D1,MAPK,Silica,B(a) P,HELF
- چکیده:
- چکیده انگلیسی: Objective: Silica and Benzo(a)pyrene are listed as carcinogens. This study aims to explore Cyclin D1, CDK4 and difference of cell cycle adjusted by MAPK signal transduction pathway in silica and B(a)P-induced malignant transformation of human embryonic lung fibroblasts. Methods: Activity of the subfamily (ERK, p38 and JNK) of mitogen-activated protein kinase (MAPK), cyclin D1 and CDK4 (cyclin dependent kinase) were evaluated using Human embryonic lung fibroblast (HELF) purchased from the cell room, basic research institute, Chinese Academy of Medical Sciences. The expression of cyclin D1 and CDK4 (cyclin dependent kinase) were measured in silica and B(a)P induced malignant using Western blot (WB) assay. Result: P-ERK and P-JNK expression increased significantly (P<0.01), while CDK4 and P-p38 expression decreased (P<0.01, P<0.05) in silica-induced malignant transformation cells compared with the control group. P-ERK, P-JNK and Cyclin D1 expression increased (P<0.01, P<0.01, P<0.05) in B(a)P-induced group compared with the control group. P-ERK and P-JNK expression decreased (P<0.01), while P-p38, Cyclin D1 and CDK4 expression increased (P<0.05, P<0.05, P<0.01) in B(a)P-induced group compared with the silica-induced group. Conclusion: MAPK and cyclin D1/CDK4 activation expressed differently in human embryo lung fibroblasts malignant transformation induced by silica and benzopyrene.
- انتشار مقاله: 08-09-1396
- نویسندگان: Huan Wang,Shuyu Xiao,Yali Tang,Ke Han,Zheng Zhang,Yulan Jin,Fuhai Shen
- مشاهده