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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: Journal of Chemical Health Risks
- نوع مقاله: Journal Article
- کلمات کلیدی: Doxorubicin,Cardiotoxicity,Embryonic stem cell,Cardiomyocyte
- چکیده:
- چکیده انگلیسی: In spite of serious cardiotoxicity side-effects, doxorubicin is frequently used for treatment of several types of cancers. Isolated human adult cardiomyocytes could be the best model for assessing drug-induced cardiotoxicity, while harvesting mature cardiomyocytes is restricted by some limitations such as biopsy size, cell numbers, viability, proliferative capacity and their disability to be passaged as a cell line. In the present study, human embryonic stem cell (hESC)-derived cardiomyocytes applied as a model for evaluation of doxorubicin cardiotoxicity. In this process, cardiogenic differentiated hESCs spheroids were exposed to different concentrations of doxorubicin for 24, 48 and 72 hours. The viability of spheroids as well as their morphology was assessed as important criterion of cardiotoxicity. Findings of the study showed that the viability of spheroids was significantly reduced at doses of 3 and 30 µM (P<0.05). Moreover, cell morphology was changed in the presence of same doses. Overall hESC-derived cardiomyocytes (hESC-CMs) could be a useful in vitro model for evaluating drug-induced toxicity.
- انتشار مقاله: 01-06-1393
- نویسندگان: Marziyeh Shalchi Tousi,Houri Sepehri
- مشاهده
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: Salvigenin,Doxorubicin,Eupatorin,HT-29,SW948
- چکیده:
- چکیده انگلیسی:
Background: Cancer persists as one of the world’s most pressing maladies. Notable points about chemotherapy are drug side effects which are almost universally encountered. Emerging knowledge focusing on mechanisms of toxicity due to chemotherapy has led to characterization of novel methods, including the exploitation of natural compounds, in combination therapies. Flavonoids are natural polyphenolic compounds that play protective roles against tumor cell development. The focus of this study was apoptotic effects of two flavonoids, eupatorin and salvigenin, in combination with doxorubicin on a cellular model of colon cancer. Method: Upon establishing a non-effective dose of doxorubicin, and effective doses of eupatorin (100μM) and salvigenin (150μM) via MTT, morphological features of apoptosis were distinguished using DAPI staining and cell cycle blockage in the sub-G1 phase. Apoptosis was determined by annexin/ PI and western blotting. ROS levels and MMP were measured to show any role of mitochondria in apoptosis. Results: Co-administration of flavonoids with doxorubicin induced apoptosis via the mitochondrial pathway as mitochondrial membrane potential and ROS production were changed. Annexin/PI analysis demonstrated that apoptosis frequency was increased with the combination treatments in colon cancer cells. Finally, the combination of these flavonoids with doxorubicin increased the Bax/Bcl-2 ratio, caspase-3 expression and PARP cleavage. Conclusion: Combination of flavonoids with doxorubicin induces apoptosis and enhances effect on cancer cells which might allow amelioration of side effects by dose lowering.- انتشار مقاله: 07-06-1396
- نویسندگان: Nazanin Namazi Sarvestani,Houri Sepehri,Ladan Delphi,Mahdi Moridi Farimani
- مشاهده