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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: Journal of Herbal Drugs
- نوع مقاله: Journal Article
- کلمات کلیدی: Antibacterial,Phenolic compounds,Foeniculum vulgare,Pathogen
- چکیده: مقدمه و هدف: رازیانه یک گیاه دارویی و آروماتیک است. آزمایشهای مختلف پزشکی در شرایط آزمایشگاهی و در شرایط زنده به طور متقاعدکنندهای توانایی ضد قارچی ، ضد باکتریایی، آنتی اکسیدانی، ضدترومبوز و فعالیت محافظتی در کبد را نشان داده است. هدف از این مطالعه بررسی فعالیت ضد باکتریایی عصاره های رازیانه بر روی باکتریهای بیماری-زا است.
روش تحقیق: به این منظور عصارههای اتانولی، متانولی و استونی (از طریق روش خیساندن) بر روی بخشهای مختلف رازیانه (گل، برگ و میوه) آماده شد و فعالیت آنها بر روی برخی گونههای باکتریایی گرم مثبت (استافیلوکوکوس آرئوس، باسیلوس سرئوس) و گرم منفی (سودوموناس آئروژینوزا، اشرشیاکلای) از طریق روش استاندارد دیسک دیفوژن امتحان شدند.
نتایج: یافتهها نشان میدهند که حساسترین باکتری نسبت به عصاره استافیلوکوکوس آرئوس و مقاومترین باکتری سودوموناس آئروژینوزا میباشد. نتایج نشان میدهد که در مورد گل رازیانه عصاره متانولی و استونی به ترتیب بیشترین و کمترین خاصیت ضد باکتریایی را دارند و عصاره اتانولی برگ رازیانه بیشترین خاصیت ضد باکتریایی را دارا هستند و در مورد میوه رازیانه عصاره متانولی و استونی به ترتیب حداکثر و حداقل فعالیت ضد باکتریایی را دارد. در کل نتیجه این مطالعه نشان میدهد که عصاره گل رازیانه نسبت به بقیه اجزا رازیانه خصوصیات ضد باکتریایی بیشتری دارد.
توصیههای کاربردی/ صنعتی: مکانیسم عمل رازیانه به علت حضور ترکیبات فنولی و ترپنوئیدی است. این ترکیبات به طور بالقوه بر روی نفوذپذیری غشای باکتریها اثر میگذارد و در نتیجه باعث مرگ سلول میشود. بنابراین عصاره رازیانه میتواند برای کنترل باکتریهای مقاوم به چندین آنتیبیوتیک مورد استفاده قرار گیرد.- چکیده انگلیسی: Background & Aim: Foeniculum vulgare Mill. (Apiaceae) is a medicinal and aromatic plant. Various pharmacological experiments in vitro and in vivo models have convincingly showed F. vulgare ability in antifungal, antibacterial, antioxidant, antithrombotic and hepatoprotective activities.
Experimental: The purpose of this study was to evaluate the antibacterial activity of extracts of Foeniculum vulgare on the number of pathogen bacteria. For this purpose, ethanol, methanol and acetone extracts (through maceration method) of different parts of the Foeniculum vulgare (flowers, leaves and fruit) were prepared and antibacterial activity was tested against some gram positive (Staphylococcus aureus, Bacillus cereus) and gram negative (Pseudomonas aeruginosa, Escherichia coli) bacterial species through standard disc diffusion method.
Results: The findings showed that the most sensitive bacteria to the extracts was Staphylococcus aureus and the most resistant bacteria was Pseudomonas aeruginosa. According to the gained results in the case of fennel flower and fruits extract, methanol and acetone extract showed the highest and lowest antibacterial activity, respectively, while in the case of fennel leaves ethanolic extract exhibited the most inhibitory effect. In general, the results of this study indicated that the extract of fennel flowers had more antibacterial properties in comparison with other parts of plant.
Recommended applications/ industries: The antibacterial activity of fennel is due to the presence of terpenoids and phenolic compounds. These compounds are potentially effective on membrane permeability of bacteria and thereby cause the cell death. Therefore, fennel extract can be used to control bacteria resistant to several antibiotics.- انتشار مقاله: 12-10-1396
- نویسندگان: َAzin Ghafarizadeh,Seyyed Mansour Seyyednejad,Hossein Motamedi,Fatemeh Shahbazi
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Pharmaceutical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: Medicinal plants,Antibacterial agents,Alcohol extracts,Ethanolic extracts,Metals assay,Methanolic extracts
- چکیده:
- چکیده انگلیسی: Unfavorable conditions can affect on secondary metabolites production in the plants. The aim of present study was to evaluate the effect of heavy metals accumulation on antibacterial potential of Chenopodium murale L., Senecio glaucus L., Malva parviflora L. and Calendula arvensis L. grown in an industrial area of steel production in Ahvaz, southwest of Iran. The cadmium (Cd), iron (Fe), manganese (Mn), nickel (Ni), lead (Pb) and zinc (Zn) values were analyzed in different parts of these medicinal herbs. The antibacterial activity of methanol and ethanol extracts of the plants was surveyed in standard disc diffusion method against Staphylococcus aureus, Bacillus subtilis, Escherichia coli and Pseudomonas aeruginosa. In addition, minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of these extracts were determined. High concentration of Cd was found in the roots of M. parviflora and S. glaucus. The concentration of other metals in these plants was within the allowed limits. Both of ethanolic and metanolic extracts exhibited the highest antibacterial effect against E. coli and P. aeruginosa. MIC and MBC values of alcoholic extracts were determined against E. coli and P. aeruginosa as the most sensitive bacterial species. It seems that antibacterial activity of studied plants is not essentially correlated with their heavy metal concentration.
- انتشار مقاله: 30-04-1395
- نویسندگان: Parzhak Zoufan,Roya Jalali,Amer Karimiafshar,Hossein Motamedi
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Pharmaceutical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی:
- چکیده:
- چکیده انگلیسی: In different folk medicine Ziziphus spina-christi is used for different purposes such as pneumonia, dysentery, scorpion stings, cough, constipation, intestinal worms and fever. The aim of this study was evaluation and comparing the antibacterial activity of methanolic and ethanolic extracts of Ziziphus spina –christi as well as subsequent structural changes in affected bacteria. For this purpose, ethanolic and methanolic extracts were prepared by 80% alcoholic solution. Antibacterial activity of these extracts was assessed using standard disc diffusion method against pathogenic bacteria. Sterile filter paper discs (6mm) were saturated by four different concentrations of each extract. The prepared discs were placed on lawn cultures of test bacteria and incubated at 37 0C for 24 h. After incubation the inhibition zone diameter around each disc was measured in millimeter. The induced changes in shape of affected bacteria were discovered using scanning electron microscopy (SEM). As a result of this study maximum inhibition zone diameter in case of methanolic extract were 18 and 14 against Staphylococcus aureus, Bacillus cereu, and in case of ethanolic extract was 15 mm for S. aureus and Proteus mirabilis. The methanolic extract of this plant was more effective against S. aureus and B. cereus than the ethanolic extract even at high concentration. While the ethanolic extract was more active on Proteus mirabilis. Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) indexes of both extracts were equal (MIC= MBC=8 mg/ml) for S. aureus. The SEM analysis revealed cell deformation and irregular shape in both S. aureus and B. cereus. These results suggest significant antibacterial activity of this plant especially against S. aureus, which its resistant strains are currently a great hazard in infection treatment. So, this plant should be considered as a potential source for finding new antibacterial agents.
- انتشار مقاله: 26-05-1394
- نویسندگان: Hossein Motamedi,Seyyed Mansour Seyyednejad,Zahra Hasannejad,Fariba Dehghani
- مشاهده
- جایگاه : پژوهشی
- مجله: Advanced Herbal Medicine
- نوع مقاله: Journal Article
- کلمات کلیدی: Antibiotic resistance,Staphylococcus aureus,Medical plants
- چکیده:
- چکیده انگلیسی: Background and aims: Infectious diseases are among the most important causes of mortality around the world and according to the WHO report 10.5 million cases of death were happened due to infections. Staphylococcus aureus is one of the most important pathogen that its infections are varied from local infections to life threatening infections. With regard to high antibiotic resistance of this agent its control and treatment is a challenge in medicine. The aim of this study was to review the effective medicinal plants against S. aureus with special attention of native plants of Iran.
Methods: A comprehensive literature review was performed on papers that have been published from 2004 till 2016 in data resources such as NCBI, Sciencedirect, Springer, Web of science and as well as local databases such as Irandoc, Islamic science citation (ISC) and magiran with special focus on those that have been reported native medicinal plants in Iran. The selected keywords were Staphylococcus aureus, medicinal plant, natural antibiotic, antibacterial plant and medicinal herbs.
Results: Different studies were found that have reported effective medicinal plants against S. aureus and were able to significantly inhibit bacterial growth. As well as different parts of these plants have been investigated for antibacterial activity and found that considerable differences are present among these parts of plants.
Conclusion: Based on the reported studies, there are different medicinal plants in Iran that can be regarded as effective source for discovery new antibacterial agents against
S. aureus and treatment of resistant strains.- انتشار مقاله: 18-06-1395
- نویسندگان: Hossein Motamedi,Elahe Soltani Fard
- مشاهده