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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: Journal of Chemical Health Risks
- نوع مقاله: Journal Article
- کلمات کلیدی: Antioxidant enzymes,lead,weight,Chlorophyll,Sodium nitroprusside
- چکیده:
- چکیده انگلیسی: Lead is a toxic heavy-metal pollutant which is hazardous to human health and the environment. Sodium nitroprusside is commonly used as a nitric oxide donor in plants. Nitric oxide is a bioactive molecule playing an important role in response to stress in plants. Weight, chlorophyll content, and the activity of catalase (EC 1.11.1. 6) and peroxidase (EC 1.11.1. 7) antioxidant enzymes of canola (Brassica napus L.) Hyola 401 in lead stress were investigated. This study tested the hypothesis that sodium nitroprusside plays an ameliorating role under lead-toxicity in canola. For seven days, thirteen-day plants were exposed to two levels of sodium nitroprusside (0 and 100 µM) and three levels of lead (0, 100, and 200 µM). Dry and fresh weight and chlorophyll content were decreased in lead stress, while sodium nitroprusside treatment increased weight and chlorophyll b in the same conditions. Lead stress increased the activity of antioxidant enzymes, and sodium nitroprusside treatment reduced their activity. The results showed that the use of sodium nitroprusside reduces lead toxicity.
- انتشار مقاله: 11-10-1397
- نویسندگان: Hossein Hamidi,Nahid Masoudian,Mostafa Ebadi,Bostan Roudi
- مشاهده
- جایگاه : پژوهشی
- مجله: Desert
- نوع مقاله: Journal Article
- کلمات کلیدی: heavy metals,Bioconcentration,A. aucheri,A. gummifer
- چکیده:
- چکیده انگلیسی: Treatment of soils contaminated with heavy metals using plant species is a method which has been widely used in the
past. This method, which is known as phytoremediation, has been applied for pollution treatment using different plant
species worldwide. The method has been used for remediation of various types of pollutants in laboratory, pilot and
industrial scales. The aim of this study was to compare the capability of heavy metal accumulation in Artemisia aucheri
and Astragalus gummifer in the Darre Zereshk deserts located in Taft, Iran. Plant and soil samples were collected from
eight stations. After acid digestion, concentration of heavy metals was measured in plant tissues and rhizosphere soil using
an ICP-OES instrument. The bioconcentration factors (BCF) and translocation factors (TF) were calculated for each plant
species. The BCF and TF in A. aucheri were higher than in A. gummifer. Therefore, it is suggested that A. aucheri has
higher capabilities in removing metals from polluted soils and hence is a better option for phytoremediation purposes.
- انتشار مقاله: 03-05-1393
- نویسندگان: M. Dalvand,Amir Hossein Hamidian,M.A. Zare Chahooki,B. Moteshare Zadeh,S.A.A. Mirjalili,E. Esmaeil Zade
- مشاهده
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: Efficacy,Gastric cancer,EOX,m-DCF,safety
- چکیده:
- چکیده انگلیسی: Introduction: One of the major challenges of advanced gastric cancer treatment is the lack of a standard regimen for patients. However, several clinical trials have shown that modified docetaxel, cisplatin, and 5-fluorouracil (m-DCF) and epirubicin, oxaliplatin, and capecitabine (EOX) regimens are superior to other regimens. Methods: This randomized, single-center clinical trial was performed on 40 patients with advanced gastric cancer. The first group received the m-DCF regimen as follows: docetaxel (40 mg/m2) on the first day; cisplatin (40 mg/m2) on the first and second days; and 5-fluorouracil (400 mg/m2) from the first to fourth day. The second group received the EOX regimen, including epirubicin (50 mg/m2) and oxaliplatin (130 mg/m2) i.v on the first day and capecitabine at a twice-daily dose of 625 mg/m2 p.o for 21 days. Treatment was applied every three weeks for a total of eight cycles in both groups. In each group, the overall and progression-free survival rates and toxicity were assessed. Results: A total of 40 patients were enrolled in this study (21 samples in the m-DCF group and 19 samples in the EOX group), 62.5% of whom were male. The median survival rate was 14.00 (95% CI: 11.82-16.18) months in the m-DCF group and 15.00 (95% CI: 9.56-20.43) months in the EOX group; however, differences between the groups were not significant. The progression-free survival rate was higher in the EOX group, although there was no significant difference between the two groups. Also, there was no significant difference regarding the side effects (e.g., toxicity) or need for supportive care between the groups. Conclusion: It seems that both m-DCF and EOX regimens are similar in terms of survival and toxicity and are recommended as first-line treatment for advanced gastric cancer with respect to the patient’s status.
- انتشار مقاله: 17-08-1398
- نویسندگان: Ahmad Ahmadzadeh,Seyed Saeid Seyedian,Armita Valizadeh,Mahin Soleimani,Pedram Nazari,Hossein Hamidi
- مشاهده
- جایگاه : پژوهشی
- مجله: Archives of Razi Institute
- نوع مقاله: Journal Article
- کلمات کلیدی: Cloning,Theileria equi,EMA-1
- چکیده:
- چکیده انگلیسی: Diversity among the pathogenic strains of Theileria equi (T. equi), a major agent of equine piroplasmosis, can affect the appropriate detection of parasite and host immunization. Production of recombinant surface proteins from an infected horse in natural endemic area provides a reliable tool for immunodiagnosis of parasite. Regarding this, the present study was targeted toward the cloning, expression, and purification of the immunogenic regions of equine merozoite antigen 1 (EMA-1 gene), as one of the most important immunodominant surface proteins in T. equi, from naturally infected horses in Iran. The immunogenic region of EMA-1 gene was amplified using the blood of infected horses. EMA-1 gene was cloned into pET26b vector. Then, recombinant plasmids (pET 26b-EMA-1) were transformed into competent E. coli BL21 (DE3) cells. Cloning was confirmed by polymerase chain reaction (PCR), restriction enzyme assays, and DNA sequence analysis. The recombinant protein was expressed using isopropyl β-D-1-thiogalactopyranoside as an inducer, purified using nickle-nitrilotriacetic acid column, and then confirmed by 10% sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and dot blot analysis utilizing Anti-His Tag antibody. Furthermore, the immunoreactivity of recombinant protein against the serum of the infected horses was evaluated using dot blot analysis. The PCR product analysis showed a 750-bp band belonging to immunogenic regions of EMA-1 gene. Sequence analysis revealed that cloned EMA-1 and protein had 94% and 97% homology to EMA-1 sequences submitted to GenBank from different countries, respectively. Restriction enzyme and sequence analyses confirmed the subcloning and correction of the orientation of inserted gene. The SDS-PAGE analysis confirmed the expression of EMA-1 protein with a 28-kDa band. The results of the dot blot analysis revealed that the horse serum containing antibody against T. equi could react with the purified recombinant protein. Purified EMA-1 protein can be used as a reliable tool for the future development of diagnostic tests or vaccines.
- انتشار مقاله: 21-02-1396
- نویسندگان: M. Ebrahimi,Hossein Hamidinejat,M. R. Tabandeh,M.H. Razi Jalali,A. Rasouli
- مشاهده