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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: Avicenna Journal of Phytomedicine
- نوع مقاله: Journal Article
- کلمات کلیدی: Klebsiella pneumoniae,Antibiotic resistance,Zataria multiflora,Extended-Spectrum-β-Lactamases (ESBLs)
- چکیده:
- چکیده انگلیسی: Objective: There are few therapeutic options for treatment of multidrug resistant Klebsiella pneumoniae isolates as a hospital infectious agent (nosocomial infection). The aim of this study was to evaluate the antibacterial activity of Zataria multiflora Boiss extracts against ESBL-producing Klebsiella pneumoniae strains.
Materials and Methods: This study was conducted on 100 K. pneumoniae isolates from two hospitals in Tehran, Iran. Antibiotic susceptibility tests were performed by Kirby-Bauer disc diffusion and microdilution broth methods and detection of ESBL was carried out according to CLSI guidelines. The blaCTX-M-15plasmid genewas detected by PCR and sequencing methods. Extracts susceptibility test was performed by broth microdilution method.
Results: Among 100 K. pneumoniae strains, 48 (48%) were ESBL positive. In this study, fosfomycin, colistin and tigecycline were more active than other antibiotics. The existence of blaCTX-M-15 was detected in 30 (62.5%) of 48 ESBL-producing isolates. The chloroformic extract showed potent activity against ESBL-producing K. pneumoniae strains (MIC50 = 1.56 mg/ml and MIC90=3.12mg/ml). The MIC50 and MIC90 (The MIC50 represents the MIC value at which ≥50% of the isolates in a test population are inhibited and the MIC90 represents the MIC value at which ≥90% of the strains within a test population are inhibited) were 3.12 and 6.25 mg/ml and 6.25 and 12.5 mg/ml for methanolic and acetonic extracts, respectively.
Conclusions: The incidence of ESBL-producing K. pneumoniae is very high. Therefore, detection of ESBL-producing K. pneumoniae isolates is of great importance in identifying drug resistance patterns in K. pneumoniae isolates and in control of infections. Zataria multiflora may have the potential to be used against multidrug resistant organisms such as clinical isolates of ESBL-producing K. pneumoniae.- انتشار مقاله: 06-02-1394
- نویسندگان: Masoud Dadashi,Ali Hashemi,Gita Eslami,Fatemeh Fallah,Hossein Goudarzi,Soroor Erfanimanesh,Arezou Taherpour
- مشاهده
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: HPV,E-cadherin,LPS,Snail,ZEB-1
- چکیده:
- چکیده انگلیسی: Objective: Cancer is one of the common diseases in the world, and cervical cancer is the fourth one. In this type of cancer, many risk factors, especially infectious diseases, such as human papilloma virus (HPV) and gram-negative bacteria can have important effects on the expression of epithelial to mesenchymal transition related genes like Snail, E-cadherin, and ZEB-1, responsible for connecting cell tissues. In this study, we have investigated the effect of Escherichia coli O111:B4 Lipopolysaccharide (LPS) on HPV positive cell line (HeLa), the expression level of the (Snail, E-cadherin, and ZEB-1), HPV oncogenes (E6, E7) and also microRNA-9, 192. Materials and Methods: HeLa cell line was treated with LPS to analyze Snail, E-cadherin, ZEB-1, E6, E7 and also microRNA-9, 192 expression by quantitative real-time PCR in 24, 48 and 72 hours. Results: Quantitative real-time PCR revealed a significant reduction in E-cadherin mRNA level at 10ug/L of LPS in three time-points and after 24 hours at 5ug/L of LPS; however, ZEB-1 at 10ug/L of LPS and Snail at 5, 10ug/L of LPS are up-regulated. E7 also illustrated a slight increase, but we did not find any relationship between E7 and LPS treatment. Additionally, there are upward trends in microRNA-9, 192 levels. Conclusion: The result of this study, LPS is able to reduce E-cadherin expression, caused by increase in repressor E-cadherin protein expression and some microRNAs, probably. Since bacterial infection can be in cervical site, it is likely to be effective in reducing the E-cadherin expression in the EMT and enhance cancer process, therefore; removing these infections by using the appropriate antibiotics may result in slowing down this process, which requires more research.
- انتشار مقاله: 28-02-1398
- نویسندگان: Shaian Tavakolian,Hossein Goudarzi,Gita Eslami,Ebrahim Faghihloo
- مشاهده
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: Iran,depression,Women,Breast cancer,mental health
- چکیده:
- چکیده انگلیسی:
Objective: Breast cancer is one of the most common cancers in women and has more severe mental and emotional effects than other types. Depression as a mental disorder affects people’s mental well-being, physical symptoms, occupational performance, and finally quality of life. The aim of this study was to determine depression levels in Iranian women with breast cancer. Methods: A systematic review study was conducted in 2017. English and Persian databases (PubMed, SCOPUS, Web of Science, Google Scholar, SID, Magiran) were searched with key words such as Depression Or Depressive Disorders AND Women AND Breast Cancer OR Tumor OR Neoplasm OR Malignancy AND Iran. Inclusion criteria allowed for cross-sectional studies conducted in Iran (published in English or Persian language journals), studies that had key words in their keywords or their titles and standard instruments for measuring depression in patients. Of the 160 publications found, eight were selected after reviewing the title, abstract and full article. Results: Age of women with breast cancer in selected studies ranged from 43.8 (SD = 47.1) to 55.9 (SD = 14.6) years. Duration of cancer in most studies was about 1-2 years. In most studies, mild levels of depression for women with breast cancer were present. However, in one study it was stated that 69.4% of participants had serious levels of depression. Conclusions: There is increase in the risk of depression in women with breast cancer. Therefore, it seems necessary to plan preventive and therapeutic measures in order to improve the mental health and quality of life of the affected patients.- انتشار مقاله: 26-07-1396
- نویسندگان: Azar Jafari,Amir Hossein Goudarzian,Masoumeh Bagheri Nesami
- مشاهده
- جایگاه : پژوهشی
- مجله: Archives of Razi Institute
- نوع مقاله: Journal Article
- کلمات کلیدی: Iran,Antibiotic resistance,Escherchia coli virulence genes,Broiler Chickens,Colibacillosis
- چکیده:
- چکیده انگلیسی: Colibacillosis caused by E.coli is one of the most common illnesses in commercial chickens and induce high financial losses in the poultry industry due to morbidity, mortality and antibiotic expenses because of overuses. E.coli can mostly cause disease as a secondary or opportunistic pathogen, while certain strains with specific virulence factors even as primary pathogen can cause colibacillosis. The aim of this study was to detect the three virulence genes including irp2, papC, and tsh in E.coli bacteria isolated from diseased broilers in Tabriz area using by multiplex polymerase chain reaction (PCR) method. The other purpose of this study was to do the in vitro drug sensitivity of the isolates using by disk diffusion method. 38 (53.5%), 25 (35.2%) and 35 (49.3%) isolates out of total 71 isolates, showed, irp2, papC and tsh genes respectively. In 16 isolates only irp2 gene, 8 isolates only papC gene, and in 13 isolates only tsh gene were detected and 12 isolates were without all these genes. In 39 isolates 2 or 3 gene were identified simultaneously. In this study most of the isolates were resistant against enrofloxacin, doxycycline, sultrim, erythromycin and danofloxacin and most of them were sensitive only to colistin and the percentage of sensitive isolates against fosfomycin, neomycin and florfenicol were 45.9%, 45.2% and 48.3% respectively. High resistance rate to most antibiotics and prevalence of some important virulence genes in APEC (avian pathogenic Escherichia coli ) isolates might be a serious hazard for both poultry industry and public health.
- انتشار مقاله: 10-07-1395
- نویسندگان: Bahram Hasani,Mansour Banani,Abbas Nouri,Hossein Goudarzi,Mohsen Akhijahani
- مشاهده