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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: Inorganic Chemistry Research
- نوع مقاله: Journal Article
- کلمات کلیدی: DNA,CT-DNA,UV-Vis absorption spectroscopy,Fluorescence spectroscopy,BSA theoretical studies
- چکیده:
- چکیده انگلیسی: In this research, the interaction of [CuL(DMF)], [NiL(DMF)] and [VOL(DMF)] (where L = ((E)-4-((2-amino-5-nitrophenylimino)methyl)benzene-1,3-diol)) complexes derived from tridentate Schiff base ligand with bovine serum albumin (BSA) and DNA was investigated via electronic absorption and fluorescence spectroscopy. The Ultraviolet-Visible (UV-Vis) spectra exhibited an isosbestic point for the complexes through titration with DNA. The experimental results showed the presence of intercalation interaction between the complexes and calf-thymus DNA (CT-DNA). The interaction of BSA protein and complexes was significant. The recorded florescence spectra of complexes interacting with DNA and BSA revealed the static quenching manner. The free binding energies of complexes and their interaction modes with DNA and BSA were determined by the molecular docking. MTT-dye reduction technique was applied to define cytotoxicity of [NiL(DMF)], [CuL(DMF)] and [VOL(DMF)] complexes against breast cancer 4T1 and colon carcinoma C26 cell lines. The [VOL(DMF)] complex had cytotoxic activity against 4T1 and C26 cell lines.
- انتشار مقاله: 09-03-1398
- نویسندگان: Morteza Dostani,Ali Hossein Kianfar,Hossein Farrokhpour,Fatemeh Abyar,Amir Abbas Momtazi-Borojeni,Elham Abdollahi
- مشاهده
- جایگاه : پژوهشی
- مجله: Physical Chemistry Research
- نوع مقاله: Journal Article
- کلمات کلیدی: time-of-flight,Mass spectrometry,Laser ionization,Alkali halides,Isotope pattern
- چکیده:
- چکیده انگلیسی: A time-of-flight mass spectrometer (TOF-MS) developed in our laboratory at Isfahan University of Technology is described here. The TOF-MS instrument uses laser as the ionization source which provides an opportunity to investigate the ions formed in laser ablation or desorption. The TOF-MS has an ionization chamber containing an accelerator and an ion lens to focus the ions into a one meter linear flight tube mass analyzer. Laser beam enters the ionization chamber through quartz window and can be focused either on the accelerator plates or in between. Solid sample may be deposited on the accelerator plate. Gas samples can also be admitted into the ionization chamber perpendicular to the accelerator axis through a leak valve. Mass spectra were obtained for gas samples as well as test solid samples. For the gas phase ionization, the laser was focused in the space between the accelerator plates while for the solid samples the laser beam was focused on the sample deposited on the repeller plate of the accelerator. Operational condition of the instrument were examined and mass calibration was achieved by measuring the flight time of the known alkali ions; Li+, Na+, K+, Cs+ and Rb+. Ions of type Alk+(Alk.Halide)n were observed for all alkali salts. In addition, spectra of alkali earth salts were obtained and assigned. An average mass accuracy of 0.016% was obtained and a mass resolution of 540 (m/Dm) was achieved for benzaldehyde (m/z = 106) as a test sample. The instrument is capable of being used for MALDI analysis.
- انتشار مقاله: 26-11-1392
- نویسندگان: Mahmoud Tabrizchi,Hossein Farrokhpour,Fateme Abyar,Hajar Azad,Maryam Mirian,Vahideh ILbeigi
- مشاهده
- جایگاه : پژوهشی
- مجله: Journal of Nanoanalysis
- نوع مقاله: Journal Article
- کلمات کلیدی: Fullerenes,infrared,C20 Isomers,UV spectra
- چکیده:
- چکیده انگلیسی: Infrared (IR) and ultraviolet (UV) spectroscopic analysis of eight structural isomers of C20 carbon nanostructures, i.e. ring, tadpole, bow-tie, dumb-bell, spiro, propellane, bowl and cage, were performed at different levels of theory including semi-empirical (AM1 and PM3), Hartree-Fock (HF/6-31++G, 6-31++G**) and density functional theory (B3LYP/6-31++G, 6-31++G**). A broad range of IR spectra (400-2700 cm-1) is covered by C-C bending vibrations (low frequency region) and C-C stretching vibrations (high frequency region) in almost all isomers. The absorption maximum (λmax) in UV spectra for bowl and cage isomers appears below 300 nm and for cyclic isomers above it. These results show that IR and UV spectra are useful tools for identification of C20 structural isomers.
- انتشار مقاله: 10-03-1396
- نویسندگان: Mehdi Zamani,Ahmad Motahari,Hossein A. Dabbagh,Hossein Farrokhpour
- مشاهده
- جایگاه : پژوهشی
- مجله: Inorganic Chemistry Research
- نوع مقاله: Journal Article
- کلمات کلیدی: DNA,CT-DNA,UV-Vis absorption spectroscopy,Fluorescence spectroscopy,BSA theoretical studies
- چکیده:
- چکیده انگلیسی: In this research, the interaction of [CuL(DMF)], [NiL(DMF)] and [VOL(DMF)] (where L = ((E)-4-((2-amino-5-nitrophenylimino)methyl)benzene-1,3-diol)) complexes derived from tridentate Schiff base ligand with bovine serum albumin (BSA) and DNA was investigated via electronic absorption and fluorescence spectroscopy. The Ultraviolet-Visible (UV-Vis) spectra exhibited an isosbestic point for the complexes through titration with DNA. The experimental results showed the presence of intercalation interaction between the complexes and calf-thymus DNA (CT-DNA). The interaction of BSA protein and complexes was significant. The recorded florescence spectra of complexes interacting with DNA and BSA revealed the static quenching manner. The free binding energies of complexes and their interaction modes with DNA and BSA were determined by the molecular docking. MTT-dye reduction technique was applied to define cytotoxicity of [NiL(DMF)], [CuL(DMF)] and [VOL(DMF)] complexes against breast cancer 4T1 and colon carcinoma C26 cell lines. The [VOL(DMF)] complex had cytotoxic activity against 4T1 and C26 cell lines.
- انتشار مقاله: 09-03-1398
- نویسندگان: Morteza Dostani,Ali Hossein Kianfar,Hossein Farrokhpour,Fatemeh Abyar,Amir Abbas Momtazi-Borojeni,Elham Abdollahi
- مشاهده