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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: Iranian Journal of Immunology
- نوع مقاله: Journal Article
- کلمات کلیدی: Cytokines,Proliferation,Regulatory T cells,17β-estradiol,Peripheral blood mononuclear cell
- چکیده:
- چکیده انگلیسی: Background: 17β-estradiol (E2) has been known to modulate immune response. Recent studies indicate that E2 at pregnancy level plays a role in regulating T cell response. Objective: To investigate the optimum dose of E2 (from 10-9 to 10-7 M) in mediating the generation of regulatory T cells (Tregs), using naïve human CD4+ T cells from healthy women. Methods: Naïve peripheral T cells were purified and conditioned with soluble anti-CD28 in anti-CD3-coated plates in the presence or absence of E2. Flow cytometry was employed to assess the expression pattern of forkhead boxP3 (FOXP3) and programmed death-1 (PD-1). Proliferation and cytokine secretions were analyzed, using XTT and ELISA assays. Results: In the presence of different doses of E2, the expression levels of anti-CD3/CD28 antibody-stimulated CD25/FOXP3 and FOXP3/PD-1 in conditioned T cells (cT) were peaked at 1 ng/ml (early pregnancy level, E2(1)) (47.14% (37.3-74.9) and 32% (27.7-52.5), respectively) and a slight, but not significant, increase after declining at 36 ng/ml (late pregnancy/pharmaceutical, E2(36)) (19.4% (15.2-24.5) and 15.8% (10.6-26.8), respectively). E2(1) cT showed a significantly reduced proliferation capacity (p<0.05) and secretion of IL-10 was enhanced in supernatants of E2(1 and 36) cT (p<0.05). In contrast to decreased TNF-a and IFN-g secretions in E2(1) cT supernatants, E2(36) stimulated TNF-a and IFN-g secretions (pConclusion: Our results indicate that the differential effect of E2 on generation of Tregs is consistent with the possibility that lower levels of pregnancy E2 are most efficient in induction of Tregs.
- انتشار مقاله: 30-03-1396
- نویسندگان: Ramina Fatemi,Ebrahim Mirzadegan,Zohreh Vahedian,Amir Hassan Zarnani,Mahmood Jeddi-Tehrani,Farah Idali
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Immunology
- نوع مقاله: Journal Article
- کلمات کلیدی: Cytokine,gene polymorphism,PCR-RFLP,Recurrent Miscarriage
- چکیده:
- چکیده انگلیسی: Background: Pro-inflammatory and anti-inflammatory cytokines and polymorphisms of their genes have been described to be involved in the pathogenesis of recurrent miscarriage (RM).
Objective: To investigate the association between RM and five polymorphisms of cytokine genes, interleukin 10 (IL-10), (-592 A/C, -819 C/T, -1082 A/G), IL-6 (-174 C/G) and IL-17 (-197 G/A) in Iranian women.
Method: Polymerase chain reaction -restriction fragment length polymorphism (PCR-RFLP) was performed to determine the frequencies of the IL-6, IL-10 and IL-17 gene polymorphisms in 85 women with RM compared with 104 healthy controls.
Results: The frequencies of IL- 10 promoter gene polymorphisms (-592 A/C and -819 C/T) were significantly higher in RM women than those in controls (p=0.003). However, no statistically significant differences were observed in the frequencies of IL-6 (-174 C/G), IL-10 (-1082 A/G) and IL-17 (-197 G/A) polymorphisms between RM women and controls.
Conclusion: These results suggest that IL-10 gene polymorphism screening might have some relevance in patients with RM, a suggestion which requires further studies.- انتشار مقاله: 14-05-1395
- نویسندگان: Motahareh Bahadori,Saeed Zarei,Amir Hassan Zarnani,Omid Zarei,Farah Idali,Reza Hadavi,Mahmood Jeddi-Tehrani
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Immunology
- نوع مقاله: Journal Article
- کلمات کلیدی: Monoclonal antibody,"a" Determinant,HBs Ag
- چکیده:
- چکیده انگلیسی: Background: The antibody response to hepatitis B surface antigen (HBsAg) controls hepatitis B virus infection. The "a" determinant of HBsAg is the most important target for protective antibody response, diagnosis and immunoprophylaxis. Mutations in this area may induce immune escape mutants and affect the performance of HBsAg assays.
Objectives: To construct clinically relevant recombinant mutant forms of HBsAg and assessment of their reactivity with anti-HBs monoclonal antibodies (MAbs).
Methods: Wild type (wt) and mutant (mt) HBsAg genes were constructed by site directed mutagenesis and SEOing PCR. The amplified genes were inserted into pCMV6-neo plasmid and transfected in CHO cell line. The expression of wt- and mtHBsAg was assessed by commercial ELISA assays and stable cells were established and cloned by limiting dilution. The recombinant mutants were further characterized using a panel of anti-HBs monoclonal antibodies (MAbs) and the pattern of their reactivity was assessed by ELISA.
Results: Ten HBsAg mutants having single mutation within the "a" determinant including P120E, T123N, Q129H, M133L, K141E, P142S, D144A, G145R, N146S and C147S together with a wt form were successfully constructed and expressed in CHO cells. Reactivity of anti-HBs MAbs with mtHBsAgs displayed different patterns. The effect of mutations on antibody binding differed depending on the amino acid involved and its location within the ‘‘a’’ determinant. Mutation at amino acids 123 and 145 resulted in either complete loss or significant reduction of binding to all anti-HBs MAbs.
Conclusion: Our panel of mtHBsAgs is a valuable tool for assessment of the antibody response to HBV escape mutants and may have substantial implications in HBV immunological diagnostics.- انتشار مقاله: 15-05-1395
- نویسندگان: Forough Golsaz Shirazi,Mohammad Mehdi Amiri,Hamed Mohammadi,Ali Ahmad Bayat,Azam Roohi,Jalal Khoshnoodi,Amir Hassan Zarnani,Mahmood Jeddi-Tehrani,Gholam Ali Kardar,Fazel Shokri
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Immunology
- نوع مقاله: Journal Article
- کلمات کلیدی: Pregnancy,Indoleamine 2,3-dioxygenase,Tolerance,Immunology
- چکیده:
- چکیده انگلیسی: Indoleamine 2,3-dioxygenase (IDO), an enzyme involved in the catabolism of tryptophan, is expressed by a variety of cells and tissues such as macrophages, dendritic cells, cells of the endocrine system and by the placenta. IFN- γ is the main inducer of this enzyme. IDO acts as an important defense mechanism of innate immunity against pathogens. It also has tumor suppressive activity and prolongs the survival of allograft. One of the interesting functions of IDO is prevention of the allogenic fetus rejection during pregnancy by inhibiting alloreactive T cells. It was shown that inhibition of IDO activity by IDO inhibitor, 1-methyl tryptophan, during mouse pregnancy causes fetal rejection. The main mechanism by which IDO protects fetus is through reducing the tryptophan level and suppressing the T cell activity in the feto-maternal interface. In this review the biological functions of IDO with emphasis on its role in allogeneic fetus protection have been discussed.
- انتشار مقاله: 15-05-1395
- نویسندگان: Amir Hassan Zarnani,Pouneh Dokouhaki,Mahmood Jeddi-Tehrani
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Immunology
- نوع مقاله: Journal Article
- کلمات کلیدی: Pregnancy,Indoleamine 2,3-dioxygenase,Tolerance,Immunology
- چکیده:
- چکیده انگلیسی: Indoleamine 2,3-dioxygenase (IDO), an enzyme involved in the catabolism of tryptophan, is expressed by a variety of cells and tissues such as macrophages, dendritic cells, cells of the endocrine system and by the placenta. IFN- γ is the main inducer of this enzyme. IDO acts as an important defense mechanism of innate immunity against pathogens. It also has tumor suppressive activity and prolongs the survival of allograft. One of the interesting functions of IDO is prevention of the allogenic fetus rejection during pregnancy by inhibiting alloreactive T cells. It was shown that inhibition of IDO activity by IDO inhibitor, 1-methyl tryptophan, during mouse pregnancy causes fetal rejection. The main mechanism by which IDO protects fetus is through reducing the tryptophan level and suppressing the T cell activity in the feto-maternal interface. In this review the biological functions of IDO with emphasis on its role in allogeneic fetus protection have been discussed.
- انتشار مقاله: 15-05-1395
- نویسندگان: Amir Hassan Zarnani,Pouneh Dokouhaki,Mahmood Jeddi-Tehrani
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Immunology
- نوع مقاله: Journal Article
- کلمات کلیدی: Pregnancy,Indoleamine 2,3-dioxygenase,Tolerance,Immunology
- چکیده:
- چکیده انگلیسی: Indoleamine 2,3-dioxygenase (IDO), an enzyme involved in the catabolism of tryptophan, is expressed by a variety of cells and tissues such as macrophages, dendritic cells, cells of the endocrine system and by the placenta. IFN- γ is the main inducer of this enzyme. IDO acts as an important defense mechanism of innate immunity against pathogens. It also has tumor suppressive activity and prolongs the survival of allograft. One of the interesting functions of IDO is prevention of the allogenic fetus rejection during pregnancy by inhibiting alloreactive T cells. It was shown that inhibition of IDO activity by IDO inhibitor, 1-methyl tryptophan, during mouse pregnancy causes fetal rejection. The main mechanism by which IDO protects fetus is through reducing the tryptophan level and suppressing the T cell activity in the feto-maternal interface. In this review the biological functions of IDO with emphasis on its role in allogeneic fetus protection have been discussed.
- انتشار مقاله: 15-05-1395
- نویسندگان: Amir Hassan Zarnani,Pouneh Dokouhaki,Mahmood Jeddi-Tehrani
- مشاهده
- جایگاه : پژوهشی
- مجله: Immunology and Genetics Journal
- نوع مقاله: Journal Article
- کلمات کلیدی: X-linked Agammaglobulinemia,Bruton's tyrosine kinase,protein expression and phosphorylation
- چکیده:
- چکیده انگلیسی: Background: X-linked (Bruton’s) agammaglobulinemia (XLA) is a rare congenital disorder with defects in early B cell development caused by mutations in the gene encoding BTK (Bruton tyrosine kinase). The aim of this study was to investigate the expression and phosphorylation of BTK protein domain in these patients.
Materials and Methods: A total of 19 patients with mutations in BTK gene were analyzed for the expression and phosphorylation of BTK protein through immunoblotting. The correlations between BTK expression and the results of immunoblotting as well as clinical and immunologic phenotypes were evaluated.
Results: Six patients showed normal expression of protein and phosphorylation of BTK and two patients had normal phosphorylation while no expression was observed. There was a significant difference between the groups of patients with normal expression of protein and those without it (p=0.01).
Conclusion: Since we identified 6 patients with normal expression and phosphorylation of BTK, and two patients with normal phosphorylation but no expression, thus more studies should be done in order to explore other aspects of the disease. Although there was not any significant correlation between the severity of clinical manifestations and BTK expression, further investigations are necessary to determine the compensatory mechanisms in XLA patients.- انتشار مقاله: 30-01-1398
- نویسندگان: Mahsa Sohani,Samaneh Delavari,Hassan Zarnani,Leila Parvaneh,Shima Rasouli,Sepideh Shahkarami,Sepideh Babaie,Fatemeh Kiaee
- مشاهده