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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: Iranian Journal of Basic Medical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: Cell differentiation,Mesenchymal stromal cell,Neuron-like cells
- چکیده:
- چکیده انگلیسی: Objective(s): Umbilical cord blood-derived mesenchymal stromal cells (UCB-MSCs) are ideally suited for use in various cell-based therapies. We investigated a novel induction protocol (NIP) to improve the neuronal differentiation of human UCB-MSCs under appropriate conditions.
Materials and Methods: This experimental study was performed in Iranian Blood Transfusion Organization (IBTO), Tehran, Iran. UCB-MSCs were cultured in DMEM medium supplemented with 10% FBS in a humidified incubator in equilibration with 5% CO2 at 37oC. For neuronal differentiation of UCB-MSCs, DMEM was removed and replaced with pre-induction medium containing RA, bFGF, EGF, and basal medium for two days. Then, NGF, IBMX, AsA, and Neurobasal medium were used for six days for this purpose. Real-time PCR was performed to analyze the neuronal differentiation of UCB-MSCs for the first time in Iran.
Results: We found that the maximum and minimum levels of gene expression were related to GFAP and nestin, respectively. In addition, our study showed that compared to other neuronal inducers, RA might play the main role in neuronal differentiation and fate of MSCs compared to other neuronal inducers.
Conclusion: Our data showed that the combination of chemical (RA, IBMX, AsA) and growth factors (NGF, EGF, bFGF) in NIP may improve the efficiency of neuronal differentiation of UCB-MSCs and may provide a new method for easy and quick application of UCB-MSCs in regenerative medicine in the future. However, the functionality of neuron-like cells must be carefully assessed in animal experiments prior to use in clinical applications.- انتشار مقاله: 10-09-1394
- نویسندگان: Hassan Rafieemehr,Maryam Kheirandish,Masoud Soleimani
- مشاهده
- جایگاه : پژوهشی
- مجله: International Journal of School Health
- نوع مقاله: Journal Article
- کلمات کلیدی: Serum Iron Status,Platelet Indices,High School Females
- چکیده:
- چکیده انگلیسی: Background: Platelet indices (PIs), including platelet count (PC), mean platelet volume (MPV), platelet distribution width (PDW), and platelet larger cell ratio (P-LCR) are used to predict clinical outcomes in patients with various medical conditions. The current study aimed at evaluating the correlation between serum iron saturation (IS) and PIs among the female students living in the Northwest of Iran. Methods: The current cross sectional study included 254 high school female (HSF) students aged 15 to 18 years in Hamadan, Iran, from September to January 2016. After completion of a questionnaire, the hematological parameters were determined using standard methods. HSF afflicted with infections and acute hemorrhage, and the ones with malignancies and inflammatory diseases were excluded. The correlation between the serum levels of IS and PIs in the female residents of Northwest of Iran was evaluated using the Pearson correlation test. Results: The reference range of PIs in healthy HSF in the Northwest of Iran was as follows: PC 241 ± 53 109/L, MPV 9.67 ± 0.88 fL, PDW 11.904% ± 1.68%, and P-LCR 22.908 ± 6.69 fL. Decreased IS and MPV resulted in increased PC in the current study population. An inverse correlation was observed between PC and IS (P < 0.05). There was no correlation between MPV, PDW, P-LCR, and IS (P > 0.05), but a linear correlation was observed between PDW and MPV (P < 0.001) as well as PDW and P-LCR (P < 0.001). Conclusions: The current study data indicated IS as one of the most important factors affecting PC. PIs can serve as a clue to the severity of iron deficiency in HSF. It is recommended to conduct complementary studies to identify prognostic and diagnostic utilities of PIs in clinical practices.
- انتشار مقاله: 11-10-1348
- نویسندگان: Hassan Rafieemehr,Mohammad Rafiee,Marzieh Mahmoodi,Ebrahim Abbasi Oshaghi
- مشاهده
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: children,Parents,risk factors,Acute Lymphoblastic Leukemia
- چکیده:
- چکیده انگلیسی: Background: Acute lymphoblastic leukemia (ALL) is the most common malignancy in children. Different
environmental factors might be effective in the occurrence of this malignancy during childhood. The aim of this study
was to find environmental risk factors in childhood ALL in Hamadan, Iran. Methods: This case-control study was
done in 2015-2018 on 125 children younger than 15 years of age suffering from ALL. Patients were matched with
130 controls with respect to age, gender, and residence location. The identification of risk factors for ALL was sought
based on the comparison of studied variables between case and control individuals. Results: A statistically significant
increased risk for ALL was found with regard to type of delivery (OR: 0.43, 95% CI: 0.20 - 0.92, p˂0.02), childcare
(OR: 4.58, 95% CI: 0.95 - 22.20, p˂0.04), birth weight (OR: 1.44, 95% CI: 1.53 - 2.21, p˂0.006), father’s education
level (OR: 2.67, 95% CI: 1.10 - 6.45, p˂0.02), and father’s job (OR: 0.2 95% CI: 0.08 - 0.51, p˂0.001). Also observed
were increased odds for ALL regarding male gender, mother’s high education level, mother’s freelance job, and medium
or high family income. No association with ALL incidence was observed for age, gender, breastfeeding, mother’s age
at pregnancy, malignancy in first- or second-degree relatives, or mother’s use of hair dye during pregnancy (p> 0.05).
Conclusion: This study showed that father’s education level, father’s job, delivery type, birth weight, and childcare
can play a role in the incidence of childhood ALL.- انتشار مقاله: 07-03-1398
- نویسندگان: Hassan Rafieemehr,Fereshteh Calhor,Hossein Esfahani,Somayeh Ghorbani Gholiabad
- مشاهده