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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: VETERINARY RESEARCH FORUM
- نوع مقاله: Journal Article
- کلمات کلیدی: Iran,Turkey,RT-PCR,Avian metapneumovirus
- چکیده:
- چکیده انگلیسی: Avian metapneumovirus (aMPV) causes diseases like rhinotracheitis in turkeys, swollen head syndrome in chickens and avian rhinotracheitis in other birds. Causing respiratory problems, aMPV adversely affects production and inflicts immense economic losses and mortalities, especially in turkey flocks. In recent years, several serological and molecular studies have been conducted on this virus, especially in poultry in Asia and Iran. The purpose of the present study was detecting and subtyping aMPV by reverse transcriptase polymerase chain reaction (RT-PCR) from non-vaccinated, commercial turkey flocks in Iran for the first time. Sixty three meat–type unvaccinated turkey flocks from several provinces of Iran were sampled in major turkey abattoirs. Samples were tested by RT-PCR for detecting and subtyping aMPV. The results showed that 26 samples from three flocks (4.10%) were positive for viral RNA and all of the viruses were found to be subtype B of aMPV. As a result, vaccination especially against subtype B of aMPV should be considered in turkey flocks in Iran to control aMPV infections.
- انتشار مقاله: 24-03-1396
- نویسندگان: Mansour Mayahi,Hassan Momtaz,Ramezan Ali Jafari,Pejman Zamani
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Fisheries Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: Nigella sativa,Satureja bachtiarica,Adjuvant,streptococcosis,lactococcosis
- چکیده:
- چکیده انگلیسی: Lactococcosis has been defined as acute septicaemia, which causes economic losses in farmed fish, especially in rainbow trout. This study was done to evaluate the effects of Satureja bachtiarica and Nigella sativa essential oils on the efficacy of lactococcosis vaccine in rainbow trout. A total number of 270 fishes with a mean average of 120 g were obtained; they were randomly divided into nine groups, each with three replicates, after two weeks of adaptation. The groups were: no injection, vaccine only, DMSO injection, vaccine with 50, 100, and 200 microgram ip injection. Two, four, and six weeks after vaccination, serological and haematological parameters were evaluated. At the sixth week, 1.7×107 cfu as LD50 96 hrs of Lactococcus garvieae were i.p injected and the relative survival percentage was calculated. The results indicated that N. sativa essence is effective on the leukocyte population as the highest number of leukocytes were found in fish receiving high concentration of N. sativa. The relative survival rate of the studied fish decreased with decreasing of the N. sativa essential oil concentration, with a significant difference with control groups (P<0.05). However, using S. bachtiarica was not significantly effective on the relative survival rate of fish. The results of this study indicated that N. sativa essential oil can be used as adjuvant for L. garvieae vaccine, since it resulted in increasing leukocytes and the relative survival rate, although S. bachtiarica was not effective on immune parameters of the studied fish.
- انتشار مقاله: 07-11-1395
- نویسندگان: Mehdi Raissy,Saeed Hashemi,Milad Roushan,Mohsen Jaafarian,Hassan Momtaz,Mehdi Soltani,Esmaeil Pirali Kheirabadi
- مشاهده
- جایگاه : پژوهشی
- مجله: International Journal of Advanced Biological and Biomedical Research
- نوع مقاله: Journal Article
- کلمات کلیدی: Iran,Seafood,Listeria spp,Listeria monocytogenes,Serotypes
- چکیده:
- چکیده انگلیسی: The objective of this study was to isolation and characterization of Listeria species and determines Listeria monocytogenes serotypes in fresh fish, shrimp, crab and lobster in Isfahan and Shahrekord, Iran. From September 2010 to April 2011, a total of 300 samples of fresh fish, shrimp, crab and lobster were obtained from randomly selected retail stores in Isfahan and Shahrekord, Iran. The samples were tested for the presence of Listeria species using a ISO 11290 protocol and then only Listeria monocytogenes isolates identified by bacteriological methods were tested by polymerase chain reaction (PCR) for serotypes detection and presences of virulence associated genes. From the total 300 samples, 23 (10.45%) fresh fish and 1 (2.5%) shrimp samples were positive for Listeria spp., but none of the lobster and crab samples were positive for Listeria species. Listeria monocytogenes only isolated from 17 fish (7.25%) and 1 shrimp (2.5%) samples while L. innocua, L. ivanovii and L. seeligeri only detected in fish samples (2 (0.9%), 3 (1.36%) and 1 (0.45%)), respectively. All of 18 isolates of L. monocytogenes showed the characteristic enhancement of hemolytic zone with S. aureus. From these positive L. monocytogenes, 66.66%, 5.55% and 27.77% had 4b, 1/2a and 1/2b serotypes, respectively. Consumption of these sea foods, either raw or undercooked, may contribute to food-borne illness due to L. monocytogenes in Iran. Also, L. monocytogenes in raw seafood may pose a health risk in kitchens if contaminating ready-to-eat food
- انتشار مقاله: 25-05-1393
- نویسندگان: Shole Yadollahi,Hassan Momtaz,Monir Doudi,Elahe Taj Bakhsh
- مشاهده
- جایگاه : پژوهشی
- مجله: Avicenna Journal of Phytomedicine
- نوع مقاله: Journal Article
- کلمات کلیدی: Punica granatum L,Pomegranate,Anti-influenza virus,Mechanisms
- چکیده:
- چکیده انگلیسی: Objective: Influenza virus, which is associated with high level of morbidity and mortality, has been recently considered a public health concern; however, the methods of choice to control and treat it are limited. Our previous study showed anti-influenza virus activity of pomegranate peel extract (PPE). In this study, the mechanism through which PPE acts against influenza virus A/Puerto Rico/8/34 (H1N1; PR8) was investigated.
Materials and Methods:Ethyl alcohol extract of pomegranate (Punica granatum L.) peel was prepared, and the action mechanism of PPE in inhibiting influenza replication was studied by time-of-drug-addition assay, virucidal activity, RNA replication, hemagglutination inhibition assay, viral mRNA expression, and western blot analysis.
Results: PPE inhibited viral polymerase activity, viral RNA replication, and viral protein expression but could not affect hemagglutination inhibition and virucidal activity. According to time-of-drug-addition assay results, PPE inhibited the virus adsorption and early steps of influenza replication.
Conclusion: This study demonstrated that the antiviral effect of PPE on influenza virus is most probably associated with inhibition of viral adsorption and viral RNA transcription.- انتشار مقاله: 16-04-1397
- نویسندگان: Mohammad-Taghi Moradi,Ali Karimi,Mahmoud Rafieian-kopaei,Mohammad Rabiei-Faradonbeh,Hassan Momtaz
- مشاهده