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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: Trends in Pharmaceutical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: oxidative stress,amino acid,Nutraceuticals,Cytoprotection,Mitochondrial cytopathies
- چکیده:
- چکیده انگلیسی: Taurine (TAU) is the most abundant free amino acid in the human body. High concentrations of this amino acid are found in tissues such as the skeletal muscle, brain, and kidney. Recently, a focus has emerged on the effects of TAU on cellular mitochondria. It has been found that TAU could positively affect this organelle by enhancing mitochondrial membrane potential, increasing ATP levels, and mitigating mitochondria-mediated ROS formation. The current study aimed to evaluate the effect of a wide range of TAU concentrations (0.01 mM-1000 mM) on mitochondrial function. Mice liver mitochondria were isolated and exposed to different concentrations of TAU (30 min). Several indices, including mitochondrial depolarization, dehydrogenases activity, permeabilization, and ATP content, were monitored. It was found that TAU supplementation significantly enhanced parameters such as mitochondrial ATP levels and mitochondrial membrane potential in comparison with the control group. Moreover, TAU prevented Ca2+-induced mitochondrial permeabilization. This amino acid revealed no significant adverse effect on isolated mitochondria even at very high and supra-physiological concentrations (e.g., 100, 250, and 500 mM). These data suggest TAU as an ideal and safe agent to protect mitochondria against toxic insults or regulating cellular function in different mitochondria-linked disorders.
- انتشار مقاله: 21-08-1398
- نویسندگان: Hamidreza Mohammadi,Mohammad Mehdi Ommati,Omid Farshad,Akram Jamshidzadeh,Mohammad Reza Nikbakht,Hossein Niknahad,Reza Heidari
- مشاهده
- جایگاه : پژوهشی
- مجله: Trends in Pharmaceutical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی:
- چکیده:
- چکیده انگلیسی: Heavy metals are environmental pollutants which pose toxicity toward biological systems. Most organs are susceptible to heavy metals-induced toxicity. Hence, finding protective agents against heavy metals-induced toxicity is valuable. The post-nuclear supernatant (PNS) has been accepted as an in vitro model for assessing xenobiotic-induced toxicity toward biological systems. Monitoring the toxic effects of a large number of xenobiotics in a short time is one of the superiorities of PNS system. The goal of the present study was to validate the PNS as an in vitro model for investigating the effect of heavy metals (Cd, Co, Cu, Fe, As, Hg, Cr, and Pb)-induced toxicity and evaluating the potential protective effects of glycine and betaine. Markers of oxidative stress including ROS formation, lipid peroxidation and glutathione content in addition of succinate dehydrogenase activity (MTT test) were monitored in the presence of heavy metals alone or in combination with glycine (1 mM) and betaine (100 µM). Our results suggest that PNS preparations can be used as an appropriate model for future investigation of xenobiotics-induced toxicity and estimation of the protective properties of different agents. Indeed, further evaluations in other experimental models could reveal the protective properties of betaine and glycine against heavy metals-induced organ injury.
- انتشار مقاله: 12-03-1397
- نویسندگان: Reza Heidari,Hamidreza Mohammadi,Asrin Ahmadi,Vahid Ghanbarinejad,Faraz Kasra,Amir Khosravi
- مشاهده
- جایگاه : پژوهشی
- مجله: Trends in Pharmaceutical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی:
- چکیده:
- چکیده انگلیسی: The xenobiotics-induced liver injury is a major clinical complication. Hence, finding hepatoprotective agents could have clinical value. Herbal medicines are a major source of biologically active chemicals which could be applied as hepatoprotective agents. The current study was designed to assess the hepatoprotective properties of Avicennia Marina (AM) extract and its different fractions. In vivo, the hepatoprotective effect of AM total extract against CCl4-induced acute liver injury was evaluated in rats, and a series of histopathological, biochemical, and oxidative stress parameters were monitored. In vitro, the protective effect of AM extract fractions (Petroleum ether, Chloroform, Ethyl acetate, and Ethanol) was evaluated on human liver hepatoma cells (HepG2). Severe elevation in serum level of liver injury biomarkers, along with liver tissue histopathological changes, lipid peroxidation, and liver tissue glutathione depletion were detected in CCl4-treated rats. On the other hand, CCl4-induced toxicity was evident in vitro by significant cell death. It was found that AM extract provided significant protection against CCL4 toxicity in vivo by decreasing serum biomarkers of liver injury and tissue markers of oxidative stress. In vitro, the protective effect of AM extract fractions (Chloroform, Ethyl acetate, and Ethanol) was evident as these fractions significantly decreased CCl4 cytotoxicity. As AM extract exhibited significant suppression of oxidative stress markers, its antioxidant effect could play a significant role in its hepatoprotective properties.
- انتشار مقاله: 29-08-1396
- نویسندگان: Omid Farshad,Reza Heidari,Hamidreza Mohammadi,Amin Reza Akbarizadeh,Mohammad M. Zarshenas
- مشاهده
- جایگاه : پژوهشی
- مجله: Trends in Pharmaceutical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: CYP1A1,Ketoconazole,omeprazol,aryl hydrocarbon receptor (AHR),FICZ
- چکیده:
- چکیده انگلیسی: Omeprazole (OMP) and ketoconazole (KTZ) have been shown to activate the AHR signaling pathway in spite of the fact that they bind to the receptor with low or no affinity. The aim of this study was to investigate whether KTZ and OMP can act as indirect activator of AHR. In order to evaluate the effects of KTZ and OMP on AHR signaling, we measured cytochromes p450 (CYP1A1) enzyme activity by ethoxyresorufin-O-deethylase (EROD) assay as endpoint. FICZ, at 1nM concentration, caused a transient elevation in the catalytic activity of CYP 1A1. KTZ and OMP were found to be inducer of CYP1A1 at concentrations above 50 µM. At early time of incubation (3hr), a dose-dependent inhibition of FICZ-induced EROD activity was seen. When OMP or KTZ were added together with FICZ, a prolonged activation of CYP1A1 was observed at later time of incubation (24h). Taken together, our findings support the earlier observation that we shown that CYP 1A1 inhibitors can act as an AHR activator though inhibition of metabolic degradation of FICZ.
- انتشار مقاله: 24-10-1395
- نویسندگان: Ali Ghaffarian-Bahraman,Nilofar Sadeghimanesh,Mona Mirzaei,Amin-Reza Akbarizadeh,Mahmoud Omidi,Hamidreza Mohammadi,Mohammad-Reza Arabnezhad,Keivan Mobini,Afshin Mohammadi-Bardbori
- مشاهده
- جایگاه : پژوهشی
- مجله: Nanomedicine Research Journal
- نوع مقاله: Journal Article
- کلمات کلیدی: Green synthesis,Myrtus Communis,Thalassemia,Silver nanoparticle,Hydrolyzable Tannins,chelation,Iron overloaded
- چکیده:
- چکیده انگلیسی: Objective(s): Nowadays, iron ions intoxication is the center of attention of interest in the management and treatment of thalassemia and different sorts of anemia associated with regular blood transfusions. Due to the major side effects of current drugs, they should be replaced with safer alternatives. Thus, in this study, functionalized hybrid silver nanoparticles, as an emerging perspective, were investigated for absorbing excess iron ions and their removal in an animal thalassemia model. Methods: The silver nanoparticles were green-synthesized using the Myrtus communis leaf methanolic extact (MC-AgNPs). The produced hybrid nano-Sorbents based on hydrolyzable tannin matrix loaded with silver nanoparticles were delivered for trying out in vivo iron chelation in thalassemia model mice in which iron-overload was imposed. MC-AgNPs and desferral were injected intraperitoneally four times a week for one month in mice with excess iron load. The total iron and Fe3+ content of serum was evaluated with the aid of plasma-atomic spectrometry microscopy and a Fe3+ ion measurement kit, respectively. Also, liver enzyme levels were evaluated by an auto-analyzer. Also, hepatic enzyme levels were appraised by using an auto-analyzer based the corresponding kits. Morphological transformations of the liver tissue were investigated by way of Prussian blue staining. Results: The mice treated with the MC-AgNPs demonstrated a significant reduction in serum iron content when compared with the iron-overload mice. MC-AgNPs revealed satisfying effectiveness to chelate excess iron in mice. Conclusions: This method could be considered as a competitive option for lowering the level of excess iron in vivo.
- انتشار مقاله: 06-06-1399
- نویسندگان: Shirin Tavakoli,Mohammad Ali Ebrahimzadeh,Fatemeh Sameni,Pourya Biparva,Hamidreza Mohammadi,Ali Ziar,Afshin Zahedi Mazandarani,Soheil Vafaeinejad,Shahram Eslami
- مشاهده
- جایگاه : پژوهشی
- مجله: Nanomedicine Research Journal
- نوع مقاله: Journal Article
- کلمات کلیدی: toxicity,Green synthesis,Myrtus Communis,Silver nanoparticle,Hydrolyzable Tannins,sub-chronic
- چکیده:
- چکیده انگلیسی: Objective(s): The use of silver nanoparticles in the field of biomedicine is increasing day by day, but less attention has been paid to its toxicity. In this paper, the ability of the silver nanoparticles produced by a green synthesis procedure to protect the liver and its effects on liver function in male mice was investigated in a sub-chronic toxicity study. Methods: The silver nanoparticles functionalized the hydrolyzable tannin fraction of Myrtus communis (MC-AgNPs) were used for testing in vivo sub-chronic toxicity in mice model. The MC-AgNPs and Ag+ were intraperitoneally injected with different doses 5 times a week over 90 days. The biochemical, hematological factors were determined using an autoanalyzer following the routine procedures. In addition, histopathological test of liver tissue in laboratory mice were examined through haematoxylin & eosin staining. Results: The obtained results showed that liver enzymes (AST, ALT, and ALP) were decreased. The mean value ± standard deviation of white blood cells, lymphocytes, red blood cells and Hb were increased, while red blood cells and hemoglobin decreased. Histopathological investigations indicated no obvious effect on hepatic cyto-architecture in the group receiving silver nanoparticles (50 mg/kg), and mild inflammation in the port space. In the groups receiving silver nanoparticles (100 and 200 mg/kg), mild inflammation, and moderate inflammation were observed in the port space and pre portal, respectively. Conclusions: The findings indicated that AgNPs could be safe even for long-term use in a therapeutic period if hybridized with active biomolecules.
- انتشار مقاله: 28-03-1399
- نویسندگان: Shirin Tavakoli,Mohammad Ali Ebrahimzadeh,Emran Habibi,Pourya Biparva,Hamidreza Mohammadi,Afshin Zahedi Mazandarani,Soheil Vafaeinejad,Ali Ziar,Shahram Eslami
- مشاهده
- جایگاه : پژوهشی
- مجله: Journal of Modern Processes in Manufacturing and Production
- نوع مقاله: Journal Article
- کلمات کلیدی: Mechanical properties,Computational Fluid Dynamics,Friction Stir Welding,PC
- چکیده:
- چکیده انگلیسی: In the present article, the effects of friction stir welding (FSW) tool plunge depth (TPD) and tool tilt angle (TTA) were investigated on the heat generation and the material flow during solid-state joining of polycarbonate (PC) sheet experimentally and simulated with computational fluid dynamics (CFD) method. Simulation results showed that increasing heat input (higher TTA and lower TPD) could increase the size of the stir zone. Results indicate that control of TPD and TTA can improve the material flow near the top surface. The temperature field in the friction stir welding of PC was anti symmetric to the welding line. Due to the results, the heat generation and temperature distribution at the advancing side were more than the retreating side in all joint conditions. According to selected parameters, a strong sample was produced at 1.2 TPD and 2.5
oTTA. This sample had 92% PC tensile strength, 89% PC flexural strength, and 86% of impact energy.- انتشار مقاله: 17-05-1398
- نویسندگان: Hamidreza Mohammadi Kuhbanani,Hesameddin Yasemi,Hamed Aghajani Derazkola
- مشاهده
- جایگاه : پژوهشی
- مجله: Journal of Theoretical and Applied Vibration and Acoustics
- نوع مقاله: Journal Article
- کلمات کلیدی: Control,Nonlinear dynamics,Multiple clearance joints,Joint friction
- چکیده:
- چکیده انگلیسی: In the current study, behavior of crank-slider mechanism with single and multiple clearance joints are analyzed. Using Lankarani-Nikravesh theory for estimating discontinuous contact forces in clearance joints, relevant systems have been mathematically modeled. Through numerical simulations, perturbations in response of mechanisms with clearance joints have been analyzed. Effects of increasing number of clearance joints have been addressed. From comparisons between responses of crank-slider mechanism with a single clearance joint and multiple clearance joints, it is concluded that perturbations intensify as the number of clearance joints in mechanism increases. Nonlinear dynamics of system are analyzed, using Poincare maps and bifurcation diagrams. Effects of joint friction on the response of the mechanism are investigated. Subsequently, a control scheme for providing continuous contact in clearance joints and maintaining a more stable mechanism is pro-posed. Obtained results demonstrate the effectiveness of proposed control method on reducing effect's of clearance and maintaining continuous contact in clearance joint
- انتشار مقاله: 22-11-1393
- نویسندگان: Morteza Dardel,Sadeq Yaqubi,Hamidreza Mohammadi Daniali,Mohammad Hassan Ghasemi
- مشاهده
- جایگاه : پژوهشی
- مجله: Journal of Midwifery and Reproductive Health
- نوع مقاله: Journal Article
- کلمات کلیدی: Menopause,Health-promoting lifestyle Behaviors,Choice theory
- چکیده:
- چکیده انگلیسی: Background & aim: Menopause is considered as a stage of life that is often encountered by all women with symptoms as hot flashes, sweating, palpitations, sleep disorders, and urinary tract problems. The improvement of health-promoting lifestyle behaviors is one of the effective measures to moderate these symptoms by facilitating the women to enhance their lifestyle behaviors through making proper choices. The aim of this study was to measure the effect of training based on choice theory on health-promoting lifestyle behaviors in menopausal women.
Methods: This two-group pretest-posttest design was conducted on 40 menopausal women within the age range of 45-55 years, referring to healthcare centers in Mashhad, Iran, in 2016. The subjects were randomly assigned into intervention and control groups using simple random sampling method. The data collection instruments included a demographic questionnaire, a menopause knowledge/attitude questionnaire, as well as Health-Promoting Lifestyle Profile II (HPLPII), which consisted of six dimensions of nutrition, physical activity, responsibility for health, stress management, interpersonal relationships, and spiritual growth. The intervention group received training in seven 90-minute sessions based on choice theory. The data were analyzed in SPSS software using descriptive statistics, Mann-Whitney U test, independent t-test, paired sample t-test, and Friedman test.
Results: According to the results, after the intervention, the mean scores of total health-promoting lifestyle behaviors (P<0.03) and responsibility for health (P=0.005) showed a significant increase in the intervention group, compared to those in the control group.
Conclusion: As the findings of this study indicated, the midwives could play an effective role in the moderation of menopausal side effects using choice theory. In this regard, the needs of the menopausal women in terms of boosting their awareness and practicing health-promoting lifestyle behaviors should be identified to deal with menopausal problems.- انتشار مقاله: 20-12-1396
- نویسندگان: Anahita Ensan,Rahele Babazadeh,Hamidreza Aghamohammadian,Monavar Afzal Aghaei
- مشاهده
- جایگاه : پژوهشی
- مجله: Journal of Midwifery and Reproductive Health
- نوع مقاله: Journal Article
- کلمات کلیدی: Pregnancy,Irrational beliefs,Prenatal coping
- چکیده:
- چکیده انگلیسی: Background & aim: Physiological changes during pregnancy cause high levels of stress in the mother. Thus, the need for maternal psychological adjustment using coping strategies is important. Coping strategies can be influenced by individual beliefs and attitudes. Therefore, this study was performed to determine the relationship between irrational beliefs and prenatal coping strategies.
Methods: This descriptive correlational study was conducted on 702 low-risk pregnant women at 35-39 weeks of gestation (gravidity: 3≥) who referred to the health centers of Mashhad, Iran, during 2015. Multi-stage random sampling was used to select the participants (stratified, cluster proportional to size). Data collection tools included Demographic and Midwifery questionnaire, Irrational Beliefs Test, and Revised Prenatal Coping Inventory. Reliability of the scales was determined with inter-class correlation coefficient. Data were analyzed using descriptive statistics, Spearman correlation, regression, and general linear models by SPSS, version 16.
Results: Total score of irrational beliefs had a significant direct correlation with avoidance coping strategies (r=0.24, P<0.001), but it had no significant correlation with planning-preparation and spiritual-positive coping strategies (P>0.05). Also, types of irrational beliefs were significantly related to the dimensions of perinatal coping strategies (P<0.001).
Conclusion: Considering that types of irrational beliefs can affect stress coping behaviors, it is necessary to include the evaluation of these two issues in prenatal screening.- انتشار مقاله: 21-09-1396
- نویسندگان: Farideh Khavari,Nahid Golmakani,Azadeh Saki,HamidReza Aghamohammadian Serbaf
- مشاهده