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- جایگاه : پژوهشی
- مجله: Journal of Kerman University of Medical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: Punica granatum var. pleniflora Anti,inflammation Antioxidant Antibacterial Cytotoxicity Murine macrophage J774A.1
- چکیده:
- چکیده انگلیسی: Background:Punica granatum var. pleniflora (PGP) has been used for thousands of years as an effective agent to treat various types of diseases. However, there are a few new evidences addressing its therapeutic effects and related mechanisms. Therefore, the aim of this study was to investigate the cytotoxic, antioxidant, antibacterial, and anti-inflammatory effects of ethanolic (ET), dichloromethane (DM), and ethyl acetate (EA) extracts of PGP. Methods: ET, DM, and EA extracts of PGP were first prepared using maceration method. Total phenolic content (TPC) of PGP was then assessed by the Folin-Ciocalteu assay, and its antioxidant capacity was determined by DPPH and FRAP methods. Furthermore, in-vitro antibacterial activity of the PGP extracts was performed. The effect of PGP on the viability of J774A.1, HUVECs, HT29, and MCF-7 cell lines was evaluated by the MTT assay. The anti-inflammatory effect of PGP was assessed in the lipopolysaccharide (LPS)-stimulated J774A.1 cell line using qRT-PCR method. Results: EA extract contained the highest phenolic content (383.3 ± 9.1 mg gallic acid/g extract) and showed the highest antioxidant activity (IC50 = 36.5 ± 2.3 µg/mL). PGP at concentration of 15 µg/mL significantly decreased the expression of COX-2 (ET) and iNOS (ET and EA) in J774A.1 cell. Also, EA showed the highest antibacterial activity. Furthermore, the PGP extracts decreased the viability of all tested cell lines in a concentration-dependent manner. As indicated by IC50, EA demonstrated the lowest IC50 for all tested cell lines. Conclusion: According to the results, antioxidant, anti-inflammatory, antibacterial, and cytotoxic effects of PGP might be driven by its phenolic compounds highly presented in the EA extract.
- انتشار مقاله: 22-04-1399
- نویسندگان: Mehrnaz Mehrabani,Mahboobeh Raeiszadeh,Hamid Najafipour,Mojde Esmaeli Tarzi,Arian Amirkhosravi,Alireza Poustforoosh,Mohammad Ali Mohammadi,Sara Naghdi,Mitra Mehrabani
- مشاهده
- جایگاه : پژوهشی
- مجله: Journal of Kerman University of Medical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: risk factors,Iran,Prevalence,Coronary Artery Disease,Kerman,Municipal regions
- چکیده:
- چکیده انگلیسی: Abstract Background and Aims: Cardiovascular diseases (CADs) are the most important causes of mortality and morbidity in the world and in Iran. These diseases are not completely curable but factors affecting them are preventable. The aim of this study was investigation of CAD risk factors in four municipal regions of Kerman city.
Methods: From the city postal codes, 250 postal codes (as clusters) were selected randomly. Research coordinator team attended households in clusters and all the eligible members were recruited to the study. The recruitment was continued to reach 24 subjects in each cluster. The sample size was 5900 individuals aged from 15 to 75 years old. The prevalence of CAD risk factors including diabetes, hypertension, overweight and obesity, low physical activity, hyperlipidaemia, cigarette and opium smoking, depression and anxiety, oral and dental hygiene were assessed.
Results: Overweight with overall prevalence of 30.5% in the city, showed significant different prevalence rates in four regions (region 1: 29.9%, region 2: 33.8%, region 3: 29.7% and region 4: 30.5%, P<0.001). Overall, diabetes and hypertension had prevalence rates of 8.1% and 11.3%, both with similar distribution in the four regions. However, in diabetic patients, the prevalence of abnormal HbA1C was 67.3%, 50.2%, 70.4% and 45.1% in regions one to four respectively (P<0.0005). The region four with 15.4% opium consumption had the highest rank among the four regions. Depression and anxiety had the highest prevalence in region 4 and the lowest in region 2 (P<0.01). The gingival score index was 72.6% in men and 68.1% in women with region four having the maximum prevalence of 80.1%.
Conclusion: Overall, the prevalence of CADs in Kerman is relatively high and differs based on the region. Therefore, it is recommended that in designing educational programs for improving health behaviours, those differences are considered. It is suggested that these educations persist on reduction of obesity, opium consumption and improvement of mental health in region 4, on weight and LDL reduction in region 2, on diabetes control in regions 1 and on mental health improvement in region 3.- انتشار مقاله: 21-08-1394
- نویسندگان: Hamid Najafipour,Majid Askaripour,Ali Hosseinzadeh,Zahra Sadeghi
- مشاهده
- جایگاه : پژوهشی
- مجله: Journal of Kerman University of Medical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: risk factors,Iran,Metabolic Syndrome,Cardiovascular diseases,Periodontal disease
- چکیده:
- چکیده انگلیسی: Background & Aims: Metabolic syndrome is composed of interrelated cardiovascular and cardiometabolic risk factors. Moreover, recent researches have illustrated the association of systemic diseases and periodontitis. Thus, the early diagnosis of this syndrome, as a cardiometabolic risk factor, seems necessary. The aim of this study was to evaluate the prevalence of metabolic syndrome in patients with periodontal disease. Methods: In this cross-sectional study, 900 subjects from the data bank of the Kerman Coronary Artery Disease Risk Study (KERCADRS), Iran, were assessed. The community periodontal index (CPI) was used as the evaluation tool for periodontal treatment needs and health condition . The control group consisted of healthy individuals. Results: The prevalence of metabolic syndrome among patients with periodontal disease was significantly higher than the healthy group (53.6% vs. 33.1%, P < 0.001). High waist circumference, triglyceride disorder, high fasting blood sugar (FBS), and high blood pressure were significantly higher in patients with periodontal disease (P < 0.05). After controlling the effect of confounding variables (age, gender and BMI), the adjusted odds ratio of existence of periodontal pockets were 1.58 (95% CI: 1.1-2.2, P = 0.012) for metabolic syndrome patients. Conclusion: Our results showed a high prevalence of cardiovascular disease risk factors among patients with periodontal disease. Therefore, screening these patients for cardiovascular disease risk factors is suggested.
- انتشار مقاله: 24-04-1395
- نویسندگان: Fatemeh Safavi,Gholamreza Yousefzadeh,Mostafa Shokoohi,Sadaf Safavi,Hamid Najafipour,Mitra Shadkam Farokhi
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Pathology
- نوع مقاله: Journal Article
- کلمات کلیدی: Histopathology,Rabbit,Rheumatoid arthritis,Chronic inflammation,Immune-arthritis
- چکیده:
- چکیده انگلیسی: Background and Objectives: The cause and pathophysiology of rheumatoid arthritis has not been fully understood and an experimental model of this disease is essential for research on the problem. In this research study, establishment and histopathological changes of chronic arthritis due to intra-articular antigen injection was used as a model of experimental rheumatoid arthritis. Materials and Methods: Thirty three New-Zeeland white rabbits were sensitized by subcutaneous injection of combination of methylated bovine serum albumin (MBSA) and Freund’s complete adjuvant (FCA) at days 1 and 14. Sensitized animals at day 28 received intra-articular injections of MBSA. At days 7, 14, 21, and 28 post-injection, excised knee joints were investigated for routine light microscopic changes. Results: It was found out that at day 7 there are fibrinous exudates in the joint space and pericapsular soft tissue, edematous synovial villi, and an intact cartilaginous site of joint. At day 14, lymphoid follicle formation at pericapsular area, short and widening of synovial villi, superficial erosion of joint cartilage (perichondritis) was observed. Thereafter, at day 21 increased secondary lymphoid follicles with active germinal centers at pericapsular areas, papillary hyperplasia of the synovial villi, thinning of the cartilaginous site of joint with mononuclear cellular infiltrates (chondritis) was noted. In addition, day 28 was demarcated by continuation of the chondritis and beginning of osteitis, granulation tissue formation (Pannus) at cartilaginous site of joint, and fibrotic changes of the synovial villi. Rare findings including pseudocyst space and palisading ranuloma at the pericapsular area was also observed. Conclusion: Antigen-induced chronic arthritis in the knee joint of the rabbit is a good experimental model to evaluate the pathogenesis and/or effects of drug interferences in the rheumatoid arthritis.
- انتشار مقاله: 06-07-1393
- نویسندگان: Shahriar Dabiri,Hamid Najafipour,Saeed Niazmand,Hamid Tabrizchi
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Medical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: risk factors,Iran,Prevalence,Coronary Artery Disease
- چکیده:
- چکیده انگلیسی: Background: The risk of disease with 1 risk factor is increased by the presence of additional risk factors. The goal of this study was to assess the prevalence of multiple coronary artery disease (CAD) risk factors among adults in Kerman, Iran, to identify the population groups most at risk.Methods: The present study included 5900 adults aged between 15 and 75 years in 2011 in Kerman, Iran. They were selected by 1-stage cluster sampling. Blood pressure, fasting blood glucose, lipids, and 6 CAD risk factors were assessed in the study population. Standardized prevalence rates were compared between the genders and age groups using the χ2 test. A P
- انتشار مقاله: 02-03-1396
- نویسندگان: Hamid Najafipour,Mahdi Afshari,Farzaneh Rostamzadeh
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Medical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: aging,Exercise,Blood-flow restriction,Nicotinic acetylcholine receptors, Neuromuscular junction
- چکیده:
- چکیده انگلیسی: Background: During the aging process, muscle atrophy and neuromuscular junction remodeling are inevitable. The present study aimed to clarify whether low-intensity aerobic exercise along with limb blood-flow restriction (BFR) could improve aging-induced muscle atrophy and nicotinic acetylcholine receptors (nAChRs) at the neuromuscular junction.Methods: Forty-eight male Wistar rats, aged 23–24 months, were randomly divided into control, sham (Sh: subjected to surgery without BFR), BFR (subjected to BFR), exercise (Ex: subjected to 10 weeks of low-intensity exercise), Sh+Ex, and BFR+Ex groups. Forty-eight hours after the last training session, the animals were sacrificed and their soleus and extensor digitorum longus (EDL) muscles were removed. The hypertrophy index was calculated, and molecular parameters were measured using western blotting. Statistical analysis was done with ANOVA using SPSS (version 20), with a P<0.05 as the level of significance.Results: The control and Sh groups showed weight gain (P=0.001), whereas the Ex, Sh+Ex, and BFR+Ex groups had significant weight loss (P<0.001). The hypertrophy index of the soleus was significantly higher in the BFR+Ex group than in the control, Sh, and BFR groups (P<0.001). BFR+Ex induced significant hypertrophic effects on the EDL (P<0.001 vs. the control, Sh, Ex, and Sh+Ex groups, and P=0.006 vs. the BFR group). BFR+Ex also increased nAChRs in the soleus (P=0.02 vs. the control and Sh groups) and the EDL (P=0.008 vs. the control and Sh groups).Conclusion: BFR plus mild exercise is a safe method with potential beneficial effects in protecting and augmenting muscle mass and nAChR clustering at the neuromuscular junction in old rats.
- انتشار مقاله: 28-02-1396
- نویسندگان: Mohammad-Ali Bahreini Pour,Siyavash Joukar,Fariborz Hovanloo,Hamid Najafipour
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Medical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: risk factors,diabetes,Metabolic Syndrome,Type 2
- چکیده:
- چکیده انگلیسی: Metabolic syndrome is highly prevalent in type 2 diabetics and is a strong risk factor for cardiovascular diseases in such patients. The aim of this study was to determine the prevalence of metabolic syndrome according to the three criteria of ATPIII, IDF and the new criteria for metabolic syndrome diagnosis in Kerman, Iran.This cross-sectional study was performed on 950 diabetic type 2 patients. Data was analyzed by independent t-test, chi-square and logistic regression using the SPSS (revision 20) software.The prevalence of metabolic syndrome in Kerman was 73.4, 64.9, and 70.4%, according to the above criteria. Fasting blood sugar, gender, triglyceride, HDL, waist circumference, and systolic blood pressure were related to the prevalence of metabolic syndrome according to the above-mentioned criteria.The prevalence of metabolic syndrome is high in type 2 diabetic patients and the above-mentioned factors exacerbate the situation
- انتشار مقاله: 28-11-1392
- نویسندگان: Zohre Foroozanfar,Hamid Najafipour,Narges Khanjani,Abbas Bahrampour,Hosseinali Ebrahimi
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Medical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی:
- چکیده:
- چکیده انگلیسی: Background: Angiotensin converting enzyme (ACE) upregulation in stromal cells of joints affected by rheumatoid arthritis may lead to higher tissue angiotensin II that is a vasoconstrictor and mitogen factor. To date, the role of angiotensin II on regulating blood flow in inflamed joints has not been studied. Methods: Acute and chronic joint inflammation was induced in rabbits by intra-articular injection of carrageenan and antigen-induced arthritis method, respectively. The ACE level of synovial fluid and the response of joint blood flow to angiotensin II, angiotensin II receptor antagonist, and the role of nitric oxide (NO) in modulation of the effects of angiotensin II on joint blood vessels were examined. Results: The synovial fluid level of ACE was significantly increased during the process of inflammation and angiotensin II increased joint vascular resistance dose-dependently in both acute and chronically inflamed joints. The angiotensin 1 receptor antagonist losartan completely blocked the vasoconstrictor effect of angiotensin II on joint blood vessels and induced vasodilatation. Nitric oxide synthase inhibitor N-omega -nitro L- arginine methyl ester (L-NAME) increased joint vascular resistance and augmented vascular response of inflamed joints to angiotensin II. Conclusion: Angiotensin II receptors in joint blood vessels are angiotensin -1 subtype, and inflammation significantly increases the activity of synovial fluid ACE. Nitric oxide plays a significant role on regulating joint blood flow and in modulation of angiotensin 1 receptor-mediated vasoconstriction of inflamed joint blood vessels.
- انتشار مقاله: 22-02-1394
- نویسندگان: Hamid Najafipour,Farnaz Nikbakht,Najmeh Sadeghi,Gholam Abbas Dehghani
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Immunology
- نوع مقاله: Journal Article
- کلمات کلیدی:
- چکیده:
- چکیده انگلیسی: Background: IGF-1 and certain other cytokines have been shown to exert inflammatory/anti-inflammatory roles in chronic joint diseases. Objective: To assess the effect of IGF-1, IL-27 and IL-35, their interaction and their receptor expression in a rheumatoid arthritis model. Methods: Freund’s adjuvant-induced chronic joint inflammation was operated on 160 male rats. Animals were divided into histopathology and receptor expression groups, each composed of 10 subgroups including; control, vehicle, IGF-1, IL-27, IL-35, their antagonists, IGF-1+IL-27 antagonist and IGF-1+IL-35 antagonist. After two weeks, vehicle or agonist/antagonists were injected into the joint space every other day until day 28 where joint histopathology was performed. The expression of IGF-1, IL-27 and IL-35 receptors were assessed by western blot analysis. Results: IGF-1 did not show pro- or anti- inflammatory functions; endogenous IL-27 and IL-35, on the other hand, exerted inflammatory effects. IL-27 and IL-35 antagonists exerted the highest anti-inflammatory effects. The total inflammation scores were 0.55 ± 0.06, 4.63 ± 0.40, 3.63 ± 0.60, 2.50 ± 0.38 and 1.63 ± 0.40 regarding control, vehicle, IGF-1 Ant., IL-27 Ant. and IL-35Ant., respectively. IGF-1 receptor expression was reduced in chronic joint inflammation and all three antagonists augmented the IGF-1 receptor expression. IL-27 and IL-35 receptors were up-regulated by chronic joint inflammation. Conclusion: Overall, the results demonstrated the pro-inflammatory role of endogenous IL-27 and IL-35 along with the over expression of their receptors in chronic joint inflammation. IL-27 and IL-35 antagonists exerted the most anti-inflammatory effects and increased IGF-1 receptor expression. These two antagonists may be potential agents for new treatment strategies in chronic joint inflammatory diseases.
- انتشار مقاله: 26-12-1396
- نویسندگان: Elham Abdi,Hamid Najafipour,Siyavash Joukar,Shahriar Dabiri,Saeed Esmaeli-Mahani,Elham Abbasloo,Nasrin Houshmandi,Abbas Afsharipour
- مشاهده
- جایگاه : پژوهشی
- مجله: International Journal of Health Policy and Management
- نوع مقاله: Journal Article
- کلمات کلیدی: Type 2 Diabetes Mellitus (T2DM),Diabetes Control,Glycosylated Hemoglobin (HbA1c),Diabetes Care,Central Obesity
- چکیده:
- چکیده انگلیسی: Background
The goal of diabetes control should be feasible in order to minimize the risk of its adverse events and to reduce its burden and cost on patients. The current study aimed to assess the status of glycemic control in male and female patients with Type 2 Diabetes Mellitus (T2DM) in Kerman, Iran.
Methods
In the present study, 500 T2DM (300 women and 200 men) from the Kerman Coronary Artery Disease Risk Study (KERCADRS), a population-based study from 2009 to 2011, were selected. Patients were >18 years old, had Fasting Blood Sugar (FBS) higher than 126 mg/dl, and had been through treatment for their diagnosed disease. All participants underwent Glycosylated Hemoglobin (HbA1c) analysis. HbA1c less than 7% was considered as good glucose control. Other metabolic indices based on American Diabetes Association (ADA) target recommendations were considered.
Results
The mean level of HbA1c in total subjects was 8.56 ± 4.72% that only 31.66% of men and 26.00% of women had controlled level of HbA1c. Total cholesterol less than 200 mg/dl was reported in 64.50% of men and 44.00% of women, High Density Lipoprotein (HDL) more than 40 mg/dl was revealed in 20.50% of men and 34.67% of women, and Low Density Lipoprotein (LDL) less than 100 mg/dl was reported in 41.50% of men and 25.33% of women. In multivariate logistic regression model, longer duration of disease and higher Waist Circumference (WC) were positively associated with uncontrolled diabetes status.
Conclusion
The findings of the present study revealed that diabetes control in T2DM was inadequate. Changing the policy of treatment in individual patient and establishing better diabetes clinic to decrease the frequency of uncontrolled T2DM are crucial. Paying attention to other affecting metabolic components such as WC in the process of T2DM management is important.- انتشار مقاله: 16-06-1393
- نویسندگان: Gholamreza Yousefzadeh,Mostafa Shokoohi,Hamid Najafipour
- مشاهده