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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: Journal of Kerman University of Medical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: Pyruvate Dehydrogenase Kinase 4 Endurance Training Metabolic Flexibility Estrogen,Related Receptor
- چکیده:
- چکیده انگلیسی: Background:Metabolic flexibility is the capacity of a system to adjust fuel (primarily glucose and fatty acids) oxidation based on nutrient availability. Pyruvate Dehydrogenase Kinase 4 (PDK4) is one of the main enzymes that play a critical role in metabolic flexibility. In current study, we examined PDK4 inhibition along with exercise training (ET) on the gene expression of Estrogen related-receptor alpha (ERRα), medium-chain acyl-CoA dehydrogenase (MCAD), carnitine palmitoyl transferase-1b (CPT-1b), peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1α), PDK4 and citrate synthase (CS) in skeletal muscle. Method:Sixty-four male Wistar rats (8 week-old) were randomly divided into 8 groups (n=8); 1- untreated control, 2- STZ-induced diabetic, 3- PDK4 inhibition, 4- endurance training (ET), 5- diabetic + PDK4 inhibition, 6- diabetic + ET, 7- PDK4 inhibition + ET, and 8- diabetic +ET + PDK4 inhibition. ERRα, MCAD, CPT-1b, PGC-1α, PDK4 and CS genes expressions were measured by Real-Time PCR and quantified by 2-ΔΔCt method. Results:ERRα, MCAD, CPT-1b, PGC-1α, PDK4, and CS expressions were significantly higher in non-diabetic+ Endurance Training group compared to the control group. The expressions of CPT-1b, MCAD and CS genes were significantly lower in the non-diabetic+ endurance training/PDK4 inhibition compared to the non-diabetic+ endurance training group, and the expressions of ERRα, CPT-1b and MCAD were significantly lower in the diabetic + PDK4 inhibition group compared to the diabetic group. Conclusion:In sum, PDK4 inhibition has negative effects on lipid metabolism in healthy rats, but in animals with diabetes, PDK4 inhibition can be used for improving lipid metabolism (over-expression of CS and PGC-1α).
- انتشار مقاله: 03-12-1398
- نویسندگان: Hamid Marefati,Yaser Masoumi-Ardakani,Saeed Shakerian,Soheil Aminizadeh,Beydolah Shahouzehi
- مشاهده
- جایگاه : پژوهشی
- مجله: Journal of Kerman University of Medical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: Aerobic training,type 2 diabetes,Angiogenesis,Resistance training,Angiostatin
- چکیده:
- چکیده انگلیسی: Background & Aims: The main long-term problems of chronic diabetes are cardiovascular complications classified as micro/macro-vascular complications. The consequences for patients include cardiovascular diseases, nephropathy, retinopathy, and so on. Due to the effect of exercise on tissue blood flow distribution and the different nature of the two types of resistance and aerobic exercises, this study aimed to compare the effect of aerobic and resistance trainings on angiostatinlevel of men with type 2 diabetes. Methods: A total of 26 men with type 2 diabetes (mean age: 64.56±11.3 years, mean weight: 68.50±5.42 kg, mean height: 150±43.162 cm and 10- year history of diabetes) referred to Kerman Diabetes Center were purposefully selected and randomly divided into two groups of aerobic and resistance trainings. After being familiarized with the research protocol, subjects did resistance exercises based on a percentage of the maximal strength (1 RM) and the endurance exercise with the intensity of percentage of maximum consumed oxygen three sessions in week (40-60 minutes each session) and for 12 weeks. Blood samples before and 24 hours after the last training session were collected. For comparison within the groups, independent t- test and for comparison between groups, repeated measure two-way ANOVA were used. Results: Serum anigiostatin level and VEGFR showed no significant difference between the two groups (P=0.116, P=0.218 respectively), but groups showed significant increase in serum angiostatinlevel and VEGFR after the intervention (P<0.05). Conclusion: The results showed that twelve weeks of resistance and aerobic exercises can improve of men with type 2 diabetes and increases their anigiostatin level but non significantly.
- انتشار مقاله: 22-04-1395
- نویسندگان: Mitra Sobhanipour,Hamid Marefati,Ahmad Gholamhosseinian Najar
- مشاهده