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- جایگاه : پژوهشی
- مجله: Iranian Journal of Veterinary Medicine
- نوع مقاله: Journal Article
- کلمات کلیدی: microscopy,Inflammatory bowel diseases,Blastocystis,demographic data,stool cultivation
- چکیده: زمینه مطالعه: بلاستوسیستیس یکی از شایعترین تکیاختههای بیهوازی موجود در روه بزرگ طیف وسیعی از مهره داران میباشد. هدف: این مطالعه با هدف بررسی شیوع بلاستوسیستیس در بیماران مبتلا به بیماری التهابی روده (IBD) انجام گردید. روشکار: جمع آوری نمونه مدفوع از 80 فرد بیمار مبتلا به IBD انجام شد. همه نمونهها در محیط کشت اختصاصی بلاستوسیستیس کشت داده شدند و جهت تشخیص بلاستوسیستیس تحت بررسی میکروسکپی قرار گرفتند. ارتباط بین اطلاعات دموگرافیک بیماران مبتلا به IBD و تکیاخته بلاستوسیستیس توسط برنامه SPSS نسخه 23 بررسی گردید. نتایج: از 80 بیمار مبتلا به IBD تعداد 52 (65%) مرد و 28 (35%) زن بودند. آلودگی به بلاستوسیستیس در 16 نمونه از 80 نمونه (20%) با روش کشت و توسط میکروسکوپ مثبت گزارش شدند. تکیاخته بلاستوسیستیس در 12 (08/23%) مرد و 4 (29/14%) زن جدا شد. با تست دقیق فیشر هیچ ارتباط معنیداری بین آلودگی به بلاستوسیستیس و جنس نشان داده نشد (0.397 = p value). همچنین هیچ ارتباط معنیداری بین آلودگی به بلاستوسیستیس و ارتباط با حیوان نیز یافت نشد. نتیجهگیری نهایی: در این مطالعه میزان آلودگی به بلاستوسیستیس در افراد مبتلا به IBD 20% گزارش شد. این میزان آلودگی نسبت به سایر گزارشات قبلی که روی میزان شیوع بلاستوسیستیس و این گروه از بیماران انجام شده بود بصورت معنیداری بالاتر گزارش شد.
- چکیده انگلیسی: Background: Blastocystis is the most common anaerobic protozoa living in the large intestine of a broad spectrum of vertebrates. Objectives: The aim of this study was to investigate the Blastocystis infection rate in inflammatory bowel disease (IBD) patients. Methods: A total of 80 stool samples were collected from IBD-proved patients. All stool samples were cultivated in Dulbecco’s modified Eagle’s medium and checked by light microscopy for detection of Blastocystis. The Correlation between demographic data of IBD patients and Blastocystis was calculated using SPSS 23. Results: The enrolled patients comprised of 52 (65%) men and 28 (35%) women. The study showed Blastocystis in 16/80 (20%) of the samples by microscopic examination and culture method. The parasite was seen among 12 (23.08%) and 4 (14.29%) men and women, respectively. No statistically significant correlation was found between infection with the parasite and animal contact. Fisher’s exact test represented that there was no correlation between gender and the presence of Blastocystis (p value= 0.397). Fisher’s exact test denoted that there was no statistical correlation between age and the presence of the parasite (p value= 0.130). Conclusions: In this study, Blastocystis was found in 20% of enrolled patients who suffered from IBD. This infection rate was significantly higher than the studies have previously described Blastocystis in this group of patients.
- انتشار مقاله: 27-09-1396
- نویسندگان: Sara Mohammad Ali Gol,Sedigheh Nabian,Fatemeh Arabkhazaeli,Hamed Mirjalali,Saied Bokaie,Mostafa Rezaeian,Mohammad Reza Zali,Hamid Asadzadeh Aghdaei
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Medical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: Cytokines,Colorectal neoplasms,Caco-2 Cells,Macrophages,Monocytes
- چکیده:
- چکیده انگلیسی: Background: Colorectal cancer (CRC) is the third most common cancer worldwide. Studies have indicated that immune cells and soluble factors play a key role in maintaining the balance between tumor-promoting inflammation and anti-tumor immunity. It has been shown that secreted cytokines from CRC cell lines could affect peripheral blood mononuclear cells (PBMCs), monocytes, and macrophages phenotypes. Macrophage infiltration has been associated with good prognosis in some cancers, but with poor prognosis in others. The present study aimed to evaluate the effect of conditioned media from CRC cells (Caco-2) on immune responses produced by PBMCs. Methods: The present study was performed at the Gastroenterology and Liver Diseases Research Institute, Shahid Beheshti University of Medical Sciences (Tehran, Iran) in 2017. Human monocytes were isolated from PBMCs by Ficoll gradient media. The co-culture of monocytes and Caco-2 conditioned media was carried out. RNA extraction and cDNA synthesis of monocytes were performed after 96 hours. Gene expression of pro- and anti-inflammatory cytokines was evaluated by real-time PCR. Statistical analysis was performed using the SPSS software (version 21.0) with the independent sample t test. P
- انتشار مقاله: 27-04-1397
- نویسندگان: Bahareh Mohebbi,Kaveh Ashtibaghaei,Mehrdad Hashemi,Mahmoud Hashemi,Hamid Asadzadeh Aghdaei,Mohammad Reza Zali
- مشاهده
- جایگاه : پژوهشی
- مجله: International Journal of Molecular and Clinical Microbiology
- نوع مقاله: Journal Article
- کلمات کلیدی: Iran,PCR,Helicobacter pylori,Colorectal cancer,gastrointestinal diseases
- چکیده:
- چکیده انگلیسی: Colorectal cancer (CRC) has defined as the uncontrolled growth of cells in the colon or rectum. The human colon contains the greatest number of microbes Therefore, microbes may play an important role in the development of colorectal cancer. Recent studies have shown an association between the microbial infection and the risk of colorectal cancer. However, the results of these studies are controversial. The nobility of the present study was to assess the prevalence of Helicobacter pylori infection in the colorectal cancer in Iran. The biopsy were done and the samples were obtained from patients suspected with colorectal cancer, from 2015 to 2017.Secondary, DNA of Helicobacter pylori was extracted by Commercial kit. PCR was performed for Helicobacter pylori glmM gene eventually, the data was analysed. A total of 86 patients in which 68 of them confirmed with CRC and 17 patients considered as controls. Of 68 biopsy specimens and control group, all of them were negative for Helicobacter pylori. It was ultimately attained that in colorectal cancer, there was no presence of Helicobacter pylori.
- انتشار مقاله: 18-06-1397
- نویسندگان: Parisa Abedi Elkhichi,Hossein Dabiri,Ehsan Nazemalhosseini Mojarad,Sama Rezasoltani,HAMID Asadzadeh Aghdaei,Ramin Pouriran,Mohammad Javad Nasiri
- مشاهده
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: Colorectal Polyp, mRNA expression,TLR9
- چکیده:
- چکیده انگلیسی: Toll-like receptor 9 (TLR9) is a cellular DNA receptor of the innate immune system which plays a pivotal role in
inflammatory response. Recently, changing expression levels of TLR9 has been observed in a wide range of cancer
cells; however, there is little information about colorectal polyps. Herein, we assessed the mRNA expression of TLR9
in different colorectal polyp types compared to normal group in order to investigate its expression level during CRC
initiation. Fifty-four biopsy samples from colorectal polyp patients and from 20 healthy subjects were collected. The
mucosal mRNA expression level of TLR9 gene was identified by real time PCR. Fold change of gene expression was
evaluated by 2-ΔΔct method. There was a significant relationship between the lower expression of TLR9 gene in the polyp
cases compared to normal individuals (P value = 0.0005), Also, decreased TLR9 mRNA expression was obtained in
adenomas in contrast to hyperplastic and normal groups (P value = 0.0008). Based on the current results, we hypothesized that aberrant surface expression of TLR9 on tumor cells may promote the growth and invasion of colorectal polyps. Further, TLR9 modulation may have an important impact on the development of novel therapeutic strategies.- انتشار مقاله: 12-08-1397
- نویسندگان: Sama Rezasoltani,Shirin Khatibi,Zahra Pezeshkiyan,Ehsan Nazemalhosseini-Mojarad,Maryam Sharafkhah,Amir Sadeghi,Hamid Asadzadeh Aghdaei,Mohammad Reza Zali
- مشاهده