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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: Iranian Journal of Biotechnology
- نوع مقاله: Journal Article
- کلمات کلیدی: Experimental Design,Amberlite Resin,Full Factorial optimization,In situ metabolic extraction,Natural product,Persipeptides
- چکیده:
- چکیده انگلیسی: Background: Drug discovery process is growing considerably due to the noteworthy resource of natural products. Persipeptides A and B are cyclopeptide antibiotics, which are produced by Streptomyces zagrosensis UTMC 1154. Although extraction of culture broth with the help of solvent has been optimized previously, no effort for in-situ extraction of persipeptides has been done yet.
Objective: To produce a high quantity of persipeptides for further drug evaluation, it is crucial to design approaches aimed at improvement of the extraction yield.
Materials and Methods: Amberlite XAD-16N was employed into the fermentation culture medium of S. zagrosensis in order to enhance the in-situ extraction of persipeptides. Effects of resin content (%), resin addition time (h), and fermentation time (day) were investigated by a two-level full factorial experimental design.
Results: The main factors of resin content (%) and the interaction of resin content (%) with resin addition time (day) were found to be important using ANOVA. The results showed the amount of 0.33 % (w.v-1) amberlite XAD-16N added at 27.2 h post-inoculation was the most effective combination to increase the efficiency of in-situ adsorption capacity of persipeptides.
Conclusions: The provided method requires 3.3 g resin and 200 mL methanol for the extraction of persipeptides from each liter of fermentation culture of S. zagrosensis in less than 15 min. Apart from cost-efficiently and simplicity, this procedure enhanced the recovery of persipeptides by 7 % and 3 times, compared to ISP2 medium without any resin after 4 and 7 days of fermentation, respectively. Therefore, this method can be regarded as a cost-efficient enhancement approach for the production of these newly-discovered metabolites before implementing the genetic manipulation or intensive media optimization, demanding considerable time and effort.- انتشار مقاله: 09-01-1397
- نویسندگان: Hamed Kazemi Shariat Panahi,Fatemeh Mohammadipanah,Farzaneh Rahmati,Aliakbar Tarlani,Javad Hamedi
- مشاهده
- جایگاه : پژوهشی
- مجله: Biofuel Research Journal
- نوع مقاله: Journal Article
- کلمات کلیدی: Biofuel,Bioethanol,Biobutanol,Microbial metabolite,Bio-jet fuel,Sustainable energy,Fuel additive
- چکیده:
- چکیده انگلیسی: Environmental deterioration, global climate change, and consequent increases in pollution-related health problems among populations have been attributed to growing consumption of fossil fuels in particular by the transportation sector. Hence, replacing these energy carriers, also known as major contributors of greenhouse gas emissions, with biofuels have been regarded as a solution to mitigate the above-mentioned challenges. On the other hand, efforts have been put into limiting the utilization of edible feedstocks for biofuels production, i.e., first generation biofuels, by promoting higher generations of these eco-friendly alternatives. In light of that, the present review is aimed at comprehensively assessing the role and importance of microorganisms such as bacteria and yeasts as catalysts for sustainable production of liquid biofuels including bioethanol, biomethanol, biobutanol, bio-ammonia, biokerosene, and bioglycerol. Various aspects of these biofuels, i.e., background, chemical synthesis, microbial production (including exploitation of wild and metabolically-engineered species), and product recovery as well as the derivatives produced from these biofuels which are used as fuel additives are thoroughly covered and critically discussed. Furthermore, the industrial features of these green liquid fuels including the industrial practices reported in the literature and the challenges faced as well as possible approaches to enhance these practices are presented.
- انتشار مقاله: 21-04-1398
- نویسندگان: Hamed Kazemi Shariat Panahi,Mona Dehhaghi,James E. Kinder,Thaddeus Chukwuemeka Ezeji
- مشاهده