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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: Iranian Journal of Pathology
- نوع مقاله: Journal Article
- کلمات کلیدی: Breast cancer,Metastasis,Gelsolin,Villin
- چکیده:
- چکیده انگلیسی: Background & Objective: Nowadays, actin-binding proteins such as Villin and Gelsolin have been considered to be associated with aggressive tumors. This study mainly aims to determine the relationship between Gelsolin and Villin genes expression and metastasis of axillary lymph nodes in patients with breast cancer.
Methods: The included population consisted of 40 confirmed cases of female breast cancer (including 20 patients with breast cancer along with axillary lymph node metastasis and 20 patients without axillary lymph node metastasis). Expression of Villin and Gelsolin genes was evaluated using Real-time PCR and pre-designed primers.
Results: The mean expression level of Villin in groups with and without axillary lymph node metastasis was 3.33±1.35 and 0.87±0.88, respectively (p <0.001). The mean Gelsolin expression levels in both groups (with and without axillary lymph node metastasis) were 4.13±2.40 and 1.00±0.35, respectively (p <0.001). The significant relationships were independent of individuals’ age.
Conclusion: Patients with axillary lymph node metastasis may express significant higher level of Villin and Gelsolin gens.- انتشار مقاله: 23-11-1398
- نویسندگان: Armin Borhan,Zohreh Nozarian,Alireza Abdollahi,Reza Shahsiah,Hadiseh Mohammadpour,Arash Borhan
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Pathology
- نوع مقاله: Journal Article
- کلمات کلیدی: Breast cancer,epithelial-mesenchymal transition,Gene,Lymphovascular invasion
- چکیده:
- چکیده انگلیسی: Background & Objective: Recent studies from gene profiling have revealed some genes that are overexpressed in the epithelial-mesenchymal transition (EMT) process and are responsible for its initiation and activation resulting in tumor progression and metastasis. The present study aimed to assess the role of genes involved in the EMT process and the association of these genes with axillary lymph node and vascular invasion in breast cancer (BC) patients.
Methods: In this case-control study, the tumor samples were initially extracted from 33 BC patients. The samples of 15 BC tissues without vascular and axillary invasion were also prepared from the biobank as a control group. RNAs from both tumor and control samples were extracted and stabilized. For assessing overexpression in tumor tissues of selected 18 genes, the real time technique was employed.
Results: There was a significant increase in MMP-2 gene fold expression in tumor cells with vascular invasion regardless of axillary involvement compared to the control group (P=0.0008) and also in the comparison of the control group with those with vascular invasion and not axillary lymph node involvement (P=0.003). In addition, gene fold expression of tissue inhibitors of metalloproteinase-1(TIMP-1) was decreased in axillary involving tumor cells compared to control group (P=0.045), and also in comparison with all samples that did not present any axillary lymph node involvements including the control group and the group with isolated vascular invasion (P=0.012).
Conclusion: Overexpression of MMP-2 and under-expression of TIMP-1 were associated with more invasive behavior in breast tumor cells.- انتشار مقاله: 01-06-1397
- نویسندگان: Alireza Abdollahi,Sepideh Jahanian,Nima Hemmati,Hadiseh Mohammadpour
- مشاهده
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: Squamous Cell Carcinoma,Gene expression,Nanog,SOX2,Oct4
- چکیده:
- چکیده انگلیسی: Objective: Oral squamous cell carcinoma (OSCC) accounts for over 90% of oral neoplasms. Finding molecular
markers for predicting prognosis is a high priority. The core transcription factors, OCT4, SOX2, and NANOG that
regulate embryonic stem cell pluripotency have been implicated in progression of various malignancies. The predictive
value of these markers and their role in the development of OSCC is still controversial. In this study, we therefore
evaluated their expression in OSCCs and adjacent non-tumor tissue. Methods: A total of 60 frozen tumor and adjacent
non-tumor tissue samples from 30 patients with OSCC were examined using quantitative reverse transcription polymerase
chain reaction (qRT-PCR). Clinical and pathological data of patients including tumor stage, lymph node metastasis
and tumor grade were also recorded. Results: Expression of SOX2 was significantly higher in adjacent non-tumor as
compared to tumor tissue (P=0.04). No statistically significant differences were found for expression of OCT4 (P=0.50)
and NANOG (P=0.68). Also, there was no significant association between expression of OCT4, SOX2, and NANOG
and clinical or pathological data (P>0.05), although slightly higher values were noted in patients without lymph node
metastasis. Conclusion: Based on the present data, decreased expression of SOX2 is correlated with carcinogenesis
in the oral cavity and development of OSCC.- انتشار مقاله: 06-10-1396
- نویسندگان: Fereshteh Baghai Naini,Pouyan Aminishakib,Alireza Abdollahi,Mahshid Hodjat,Hadiseh Mohammadpour,Neda Kardouni Khoozestani
- مشاهده