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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: Iranian Journal of Veterinary Research
- نوع مقاله: Journal Article
- کلمات کلیدی: Cattle,FMD,Horn lesion
- چکیده: بیماری تب برفکی (FMD)، یک بیماری عفونی اصلی در حیوانات زوج سم میباشد. نشانههای کلاسیک بیماری در گاو شامل وزیکلهای اپیدرمی میباشد که به طور عمده در اطراف حفره بینی-دهانی، سمها، و سر پستان حضور مییابد. هدف از این گزارش، مستند کردن یک فرم بالینی غیر رایج از بیماری میباشد که شامل وقوع ضایعات وزیکلی کلاسیک بیماری در ناحیه بافت زایای شاخ میباشد. در طی شیوع بیماری در سال 2013 در ایران، بررسی میدانی، معاینه بالینی، و نمونهبرداری از گلههای درگیر در استان قم انجام شد. نمونهها از بافت پوششی دهان و بافت زایای شاخ برای مطالعه ویروس شناسی و هیستوپاتولوژی جمعآوری شد. آزمون الایزا (ELISA) بر روی نمونههای بافتی انجام شد و سپس آزمون واکنش زنجیرهای پلیمراز (PCR) بر روی نمونههای مثبت صورت پذیرفت و سویه ویروس A05 شناسایی گردید. تمام نمونههای بافت شاخ برای ویروس بیماری تب برفکی (FMDV) مثبت شدند. حیوانات با ضایعات شاخ همگی از گلههای پرواری با سن کمتر از 12 ماه بودند و با شدت بیشتری درگیر بودند. از آنجایی که سویه یکسان ویروس در گلههای شیری مجاور منجر به ضایعات مشابهی نشده بود، نتیجهگیری شد که وقوع ضایعات شاخی، به جای سویه ویروس، شاید بیشتر با عوامل میزبان در ارتباط باشد.
- چکیده انگلیسی: Foot-and-mouth disease (FMD) is a major infectious disease in livestock. The common clinical signs in cattle include epidermal vesicles that are majorly distributed around oronasal cavity, feet and teats. The aim of this report is to document an uncommon clinical form of the disease which comprises the occurrence of classic vesicular lesion in a rarely observed location of the horn vegetative tissue. During Iran’s outbreak of FMD in 2013, field investigation, clinical examination and sampling from the affected herds in Qom province were performed. Specimens of mouth epithelium and horn vegetative tissue were collected for virology and histopathologic study. All the samples collected from horns were positive for foot-and-mouth disease virus (FMDV) in both enzyme linked immunosorbent assay (ELISA) and polymerase chain reaction (PCR) tests, and the strain of the virus was identified as A05. Surprisingly, all the animals with horn lesion came from beef herds, were less than 12 months old and had more severe signs of the systemic disease. Since the same strain of virus did not cause similar lesions in surrounding dairy cows, it was concluded that occurrence of horn lesions may be more associated with host factors rather than virus strain.
- انتشار مقاله: 28-12-1395
- نویسندگان: M. R. Mohebbi,S. M. Barani,H. Mahravani
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Veterinary Research
- نوع مقاله: Journal Article
- کلمات کلیدی: Cattle,Foot-and-mouth disease,Montanide ISA 61 VG,Oil adjuvant,Vaccine potency
- چکیده: تب برفکی یک بیماری مهم در حیوانات زوج سم میباشد. در حال حاضر به طور گستردهای واکسنهای حاوی ذره ویروسی کامل غیر فعال شده جهت واکسیناسیون پیشگیرانه برای مبارزه با تب برفکی استفاده میگردد. یاورها به عنوان یک عامل مهم در برانگیختن پاسخ ایمنی و کارآیی واکسنهای غیر فعال میباشند. مونتاناید ISA 61 VG یک یاور بر پایه امولسیون روغنی جدید و آماده برای فرمولاسیون میباشد که توسط شرکت SEPPIC فرانسه با توانایی زیاد در ایجاد پاسخ ایمنی که لازمه محافظت بالینی بر علیه تب برفکی میباشد تهیه گردیده است. در این مطالعه اقدام به مقایسه کارآیی واکسن تب برفکی حاوی یاور روغنی جدید ISA 61 VG و ساپونین با واکسن حاوی ژل آلومینیوم هیدروکساید و ساپونین گردیده. هر دو واکسن با مقادیری یکسان از آنتی ژن O2010/IR تهیه گردید. دو گروه 15 راسی گوساله حساس با دوزهای مختلف واکسن (دوز کامل، 3/1 دوز و 9/1 دوز) یک بار واکسینه شدند تا قدرت 50% محافظت کنندگی (PD50) هر یک از واکسنها توسط آزمون چالنج با ویروس بیماریزا و مشابه با آنتی ژن موجود در واکسن محاسبه گردد. میانگین تیتر آنتی بادی برای هر یک از واکسنها در روزهای صفر، هفت، چهارده و بیست و یک پس از واکسیناسیون از طریق آزمایش میکرونوترالیزاسیون اندازهگیری شد. میانگین لگاریتمی تیتر آنتی بادی سرمی واکسن جدید روغنی برابر با 91/2 در مقایسه با واکسن ژل آلومینیوم هیدروکساید 44/2 (P=0.1782) گردید و توانست به میزان 19% ایمنی هومورال را بهبود بخشد. قدرت واکسن حاوی یاور روغنی جدید نیز توانسته قدرت محافظت کنندگی بسیار بالاتری PD50=10.05 در مقایسه با واکسن حاوی ژل آلومینیوم هیدروکساید PD50=4.171 را ایجاد نماید. بر اساس نتایج به دست آمده واکسن فرموله شده با یاور جدید روغنی ISA 61 VG میتواند جایگزین مناسبی برای واکسیناسیون رایج و اضطراری در مناطق آلوده با تب برفکی باشد.
- چکیده انگلیسی: Foot-and-mouth disease is an important viral disease of cloven-hoofed animals. Inactivated whole particle virus vaccines are still widely used in prophylactic vaccination campaigns. The choice of adjuvant is a very important factor in enhancing immune responses and the efficacy of inactivated vaccines. Montanide ISA 61 VG is a new ready-to-use mineral oil-based adjuvant developed by SEPPIC Inc. (SEPPIC, France) with high-potential immune responses needed for clinical protection against FMD infection. In this study, we compared the efficacy of two FMD vaccines either formulated with the new oil-based adjuvant ISA 61 VG and saponin, or with aluminum hydroxide gel and saponin. Both vaccines contained the same antigen payloads of O2010/IR. Two groups of 15 naive cattle received a single vaccination with different doses (full dose, 1/3 dose and 1/9 dose) to calculate their PD50 (50% protective dose) after being challenged with the homologous virulent virus. The mean neutralizing antibody titer was determined at 0, 7, 14 and 21 days after vaccination, measured by a micro neutralization test. The new vaccine improved humoral immune responses by 19%, while inducing a higher geometric mean. The titer for neutralizing antibodies was 2.91 log10 compared to the alum-gel based adjuvant vaccine which was 2.44 log10 (P-value=0.1782). The new vaccine showed a PD50 value of 10.05 as compared to a PD50 value of 4.171, respectively. According to the results, the FMD vaccine formulated with the new oil adjuvant, ISA 61 VG, shows potential as an alternative vaccine for routine and emergency vaccinations in the FMD enzootic region.
- انتشار مقاله: 07-04-1394
- نویسندگان: A. Khorasani,O. Madadgar,H. Soleimanjahi,H. Keyvanfar,H. Mahravani
- مشاهده
- جایگاه : پژوهشی
- مجله: Archives of Razi Institute
- نوع مقاله: Journal Article
- کلمات کلیدی: genotyping,Foot and Mouth Disease virus,serotype A,phylogenetic
- چکیده:
- چکیده انگلیسی: The foot-and-mouth disease virus (FMDV) with a wide variety of genomes and complicated biology is one of the infectious agents that put the lives of animals at risk. Therefore, to introduce suitable strains for vaccine production, it is essential to constantly evaluate genetic changes of circulating viruses in field. Within 2014-2015, a total of 126 clinical specimens consisting of epithelial tissue and vesicular fluid from tongue, dental pad, and hoofs suspected of FMD virus were submitted to the Reference Laboratory for FMD in Razi Vaccine and Serum Research Institute, and 86 of them were identified as FMD virus type A using sandwich Enzyme-Linked Immunosorbent Assay (ELISA). This virus was isolated from 42 samples from 16 provinces using cell culture. Firstly, the coding region that produces the main part of viral capsid was amplified by Polymerase chain reaction (PCR). This part of the genome by 800 bp length was related to the 1D gene that synthesizes the VP1 protein. The phylogenetic analysis of VP1 coding region determined two distinct genotypes with more than 15% nucleotide differences. The first cluster consisted of closely related viruses registered in the GeneBank of neighboring countries, including Afghanistan, Pakistan, and Turkey. All samples in Cluster1 were determined as relative viruses with genotype Iran-05. In-vitro serological examination indicated an antigenic relationship between Cluster 1 viruses and routine vaccine strain (A-IRN-2013). The second cluster with only two members was genetically far from earlier ones and could be considered a separate genotype. Furthermore, it was revealed that cluster 2 has not been previously reported in Iran. Genetic tracing indicated that these viruses might have been originated from circulating viruses from India. Antigenic evaluation exhibited that this group could not be cross-protected by the routine vaccinal strain (A-IRN-2013) used during the research period.
- انتشار مقاله: 09-10-1397
- نویسندگان: S. M. Azimi,H. Mahravani,M. Lotfi
- مشاهده
- جایگاه : پژوهشی
- مجله: Archives of Razi Institute
- نوع مقاله: Journal Article
- کلمات کلیدی: Antibody,Cattle,Cut off,Enzyme-linked immunosorbent assay,Foot and Mouth disease,Serum neutralization test
- چکیده:
- چکیده انگلیسی: Foot and mouth disease (FMD) is a contagious animal disease that causes irreparable damage to the economy of countries, including Iran in which this disease is a native one. Among the ways to combat FMD are vaccination and slaughter. Due to the specific situation of Iran, it is not possible to kill infected animals. Therefore, vaccination is the most important way to fight this disease. Serum neutralization test (SNT) and enzyme-linked immunosorbent assay (ELISA) are two main methods to evaluate the safety and calculate antibody titer. In this study, an indirect ELISA test was developed based on the coating of a complete viral particle (140s) which made it possible to determine antibody. In addition, serotype and viral type were determined without the need for time-consuming and complex molecular tasks, including gene expression. Moreover, in case of a new epidemic, a new epidemic condition can be detected using a serum antibody method. However, the coating of the complete viral particle leads to virus purification as well as the conjugated anti-immunoglobulin antibody testing of the same animal. In this study, the SNT was used as a gold standard test to determine the serum antibody level and compare its results with indirect ELISA method to determine the sensitivity and specificity of the indirect ELISA. To measure the anti-virus antibody rate of FMD (type A2013) through receiver operating characteristic analysis with 100% sensitivity and the specificity of 90%, the routine formulas were utilized using 100 % and 82%sensitivity and specificity, respectively. In this study, the cutoff value for the optical density was obtained at 0.3 and there was a significant difference between the vaccinated animals and the unvaccinated ones in terms of antibody level against the A2013 type. This indicates the correctness of the test and the accurate and proportional antibody detection against the understudy viral types of FMD.
- انتشار مقاله: 19-09-1396
- نویسندگان: F. Malekdar,H. Mahravani,A. Sedigh,M. Akbarzadegan
- مشاهده
- جایگاه : پژوهشی
- مجله: Archives of Razi Institute
- نوع مقاله: Journal Article
- کلمات کلیدی: Antibody,Cattle,Cut off,Enzyme-linked immunosorbent assay,Foot and Mouth disease,Serum neutralization test
- چکیده:
- چکیده انگلیسی: Foot and mouth disease (FMD) is a contagious animal disease that causes irreparable damage to the economy of countries, including Iran in which this disease is a native one. Among the ways to combat FMD are vaccination and slaughter. Due to the specific situation of Iran, it is not possible to kill infected animals. Therefore, vaccination is the most important way to fight this disease. Serum neutralization test (SNT) and enzyme-linked immunosorbent assay (ELISA) are two main methods to evaluate the safety and calculate antibody titer. In this study, an indirect ELISA test was developed based on the coating of a complete viral particle (140s) which made it possible to determine antibody. In addition, serotype and viral type were determined without the need for time-consuming and complex molecular tasks, including gene expression. Moreover, in case of a new epidemic, a new epidemic condition can be detected using a serum antibody method. However, the coating of the complete viral particle leads to virus purification as well as the conjugated anti-immunoglobulin antibody testing of the same animal. In this study, the SNT was used as a gold standard test to determine the serum antibody level and compare its results with indirect ELISA method to determine the sensitivity and specificity of the indirect ELISA. To measure the anti-virus antibody rate of FMD (type A2013) through receiver operating characteristic analysis with 100% sensitivity and the specificity of 90%, the routine formulas were utilized using 100 % and 82%sensitivity and specificity, respectively. In this study, the cutoff value for the optical density was obtained at 0.3 and there was a significant difference between the vaccinated animals and the unvaccinated ones in terms of antibody level against the A2013 type. This indicates the correctness of the test and the accurate and proportional antibody detection against the understudy viral types of FMD.
- انتشار مقاله: 19-09-1396
- نویسندگان: F. Malekdar,H. Mahravani,A. Sedigh,M. Akbarzadegan
- مشاهده