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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: Gene expression,immunohistochemistry,Minichromosome maintenance complex,MCM,cervical carcinogenesis
- چکیده:
- چکیده انگلیسی: Objective: Minichromosome maintenance complex (MCM) proteins are essential for the process of DNA replication
and cell division. This study aimed to evaluate MCM genes expression profiles and MCM2 protein in HPV-associated
cervical carcinogenesis. Methodology: MCM2, 4, 5 and 7 genes expression profiles were evaluated in three cervical
tissue samples each of normal cervix, human papillomavirus (HPV)-infected low grade squamous intraepithelial
lesion (LSIL), high grade squamous intraepithelial lesion (HSIL) and squamous cell carcinoma (SCC), using Human
Transcriptome Array 2.0 and validated by nCounter® PanCancer Pathway NanoString Array. Immunohistochemical
expression of MCM2 protein was semi-quantitatively assessed by histoscore in tissue microarrays containing 9 cases of
normal cervix, 10 LSIL, 10 HSIL and 42 cases of SCC. Results: MCM2, 4, 5 and 7 genes expressions were upregulated
with increasing fold change during the progression from LSIL to HSIL and the highest in SCC. MCM2 gene had the
highest fold change in SCC compared to normal cervix. Immunohistochemically, MCM2 protein was localised in
the nuclei of basal cells of normal cervical epithelium and dysplastic-neoplastic cells of CIN and SCC. There was a
significant difference in MCM2 protein expression between the histological groups (P = 0.039), and histoscore was the
highest in HSIL compared to normal cervix (P = 0.010). Conclusion: The upregulation of MCM genes expressions in
cervical carcinogenesis reaffirms MCM as a proliferative marker in DNA replication pathway, whereby proliferation of
dysplastic and cancer cells become increasingly dysregulated and uncontrolled. A strong expression of MCM2 protein
in HSIL may aid as a concatenated screening tool in detecting pre-cancerous cervical lesions.- انتشار مقاله: 17-03-1398
- نویسندگان: Gurjeet Kaur,Shandra Devi Balasubramaniam,Yung Jen Lee,Venugopal Balakrishnan,Chern Ein Oon
- مشاهده
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: nasopharyngeal carcinoma risk factors,Dietary cancer risk in Asian,Salted fish consumption as a cancer risk Factor,Lifestyle risk factors,Tobacco Smoking and alcohol consumption
- چکیده:
- چکیده انگلیسی: Objective: Risk factors of nasopharyngeal carcinoma (NPC) have been linked with diets, life style and viral
infections. NPC is more rampant in Asian populations than non-Asian countries. Our study aims to assess the validity
of the suggestions provided by multiple case control studies demonstrating that salted fish consumption, smoking and
alcohol consumption are associated with the risk of NPC in Asia. Methods: Search for related literature on salted fish,
smoking and alcohol consumption were performed via Science Direct, PubMed databases and Google Scholar. Articles
included in this study were from 2009 to 2017, with specific focus on salted fish, smoking and alcohol consumption
as risk factors of NPC. This study excluded all articles published prior to 2009 and articles involving other cancers.
Data were extracted independently by two different researchers and harmonized. Meta-analysis was conducted on the
obtained data, by using R package Meta to create funnel and forest plots. Results: The meta-analysis revealed that
salted fish, smoking and alcohol consumption were significantly associated to NPC risk with random effect model score
showing OR of 1.41 at 95% confidence interval (CI) of 1.13-1.75 (P<0.01), OR of 1.89 at 95 % CI of 1.49 – 2.38, and
OR: 1.42 at 95 % CI of 1.23 – 1.65 respectively. Our results also revealed significant association of salted meat, salted
vegetables, house type, wood dust exposure associated with NPC risk with p values less than 0.05. Conclusion: This
study proposes that salted fish intake, smoking and alcohol consumption might be linked to NPC risk in Asians. Further
studies are necessary to ascertain the molecular mechanisms and clarify if the associated path that could function as
therapeutic target.- انتشار مقاله: 15-06-1398
- نویسندگان: Simon I Okekpa,Rabiatul Basria S M N Mydin,Ernest Mangantig,Nor Syaffaf Amaliana Azmi,Siti Nur Syahirah Zahari,Gurjeet Kaur,Yusri Musa
- مشاهده