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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: Nanomedicine Journal
- نوع مقاله: Journal Article
- کلمات کلیدی: PLGA nanoparticles,Controlled release profile,FITC-HSA,Initial burst release,Protein delivery
- چکیده:
- چکیده انگلیسی: Objective(s):
Despite of wide range applications of polymeric nanoparticles in protein delivery, there are some problems for the field of protein entrapment, initial burst and controlled release profile.
Materials and Methods:
In this study, we investigated the influence of some changes in PLGA nanoparticles formulation to improve the initial and controlled release profile. Selected parameters were: pluronic F127, polysorbate 80 as surfactant, pH of inner aqueous phase, L/G ratio of PLGA polymer, volume of inner aqueous phase and addition of polyvinylpyrrolidone as an excipient. FITC-HSA was used as a model hydrophilic drug. The nanoparticles were prepared by nanoprecipitation.
Results:
Initial release of FITC-HSA from PLGA-tween 80 nanoparticles (opt-4, 61%) was faster than control (PLGA-pluronic) after 2.30 h of incubation. Results showed that decrease in pH of inner aqueous phase to pI of protein can decrease IBR but the release profile of protein is the same as control. Release profile with three phases including a) initial burst b) plateau and c) final release phase was observed when we changed volume of inner aqueous phase and L/G ratio in formulation. Co-entrapment of HSA with PVP and pluronic reduced the IBR and controlled release profile in opt-19. Encapsulation efficiency was more than 97% and nanoparticles size and zeta potentials were mono-modal and -18.99 mV, respectively.
Conclusion:
In this research, we optimized a process for preparation of PLGA-PVP-pluronic nanoparticles of diameter less than 300 nm using nanoprecipitation method. This formulation showed a decreased initial burst and long lasting controlled release profile for FITC-HSA as a model drug for proteins.- انتشار مقاله: 31-03-1394
- نویسندگان: Shahryar Shakeri,Rasoul Roghanian,Giti Emtiazi,Cesare Errico,Emo Chiellini,Federica Chiellini
- مشاهده
- جایگاه : پژوهشی
- مجله: Nanomedicine Journal
- نوع مقاله: Journal Article
- کلمات کلیدی: PLGA nanoparticles,Controlled release profile,FITC-HSA,Initial burst release,Protein delivery
- چکیده:
- چکیده انگلیسی: Objective(s):
Despite of wide range applications of polymeric nanoparticles in protein delivery, there are some problems for the field of protein entrapment, initial burst and controlled release profile.
Materials and Methods:
In this study, we investigated the influence of some changes in PLGA nanoparticles formulation to improve the initial and controlled release profile. Selected parameters were: pluronic F127, polysorbate 80 as surfactant, pH of inner aqueous phase, L/G ratio of PLGA polymer, volume of inner aqueous phase and addition of polyvinylpyrrolidone as an excipient. FITC-HSA was used as a model hydrophilic drug. The nanoparticles were prepared by nanoprecipitation.
Results:
Initial release of FITC-HSA from PLGA-tween 80 nanoparticles (opt-4, 61%) was faster than control (PLGA-pluronic) after 2.30 h of incubation. Results showed that decrease in pH of inner aqueous phase to pI of protein can decrease IBR but the release profile of protein is the same as control. Release profile with three phases including a) initial burst b) plateau and c) final release phase was observed when we changed volume of inner aqueous phase and L/G ratio in formulation. Co-entrapment of HSA with PVP and pluronic reduced the IBR and controlled release profile in opt-19. Encapsulation efficiency was more than 97% and nanoparticles size and zeta potentials were mono-modal and -18.99 mV, respectively.
Conclusion:
In this research, we optimized a process for preparation of PLGA-PVP-pluronic nanoparticles of diameter less than 300 nm using nanoprecipitation method. This formulation showed a decreased initial burst and long lasting controlled release profile for FITC-HSA as a model drug for proteins.- انتشار مقاله: 31-03-1394
- نویسندگان: Shahryar Shakeri,Rasoul Roghanian,Giti Emtiazi,Cesare Errico,Emo Chiellini,Federica Chiellini
- مشاهده
- جایگاه : پژوهشی
- مجله: Progress in Biological Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: classification,Pigment,Microorganism,phylogenetic marker,Fourier transform infrared (FTIR) spectroscopy
- چکیده:
- چکیده انگلیسی: The identification, differentiation and classification of microorganisms have been subjects of research for many years. Recently, Fourier transform infrared (FTIR) spectroscopy techniques have gained attention in the characterization and classification of microorganisms based on biochemical profiles and cell structure characteristics. In the present study, the characterization and differentiation of pigmented photoreceptor-producing microorganisms using FTIR spectroscopy was carried out. For this purpose some microorganisms were isolated from different environments, of which three photoreceptor-producing bacteria were selected to limit the scope of the study to one phenotypic characteristic. Genomic relatedness among the isolated strains was investigated and it was shown that these strains had similarities to the Kushneria marisflavi, Halobacillus halophilus and Halobacillus faecis species. In addition, Halobacterium salinarum was investigated as a typical representative photoreceptor-producing archaeon. Spectra (500-4000 cm-1) of the intact cells and crude extracted pigments were recorded on an FTIR spectrometer and compared with each other. The similarities among the spectra were evaluated using hierarchical cluster analysis and compared with the phylogenic tree based on genomic study. Our results demonstrate that hierarchical clustering based on extracted pigments shows separation of strains more distinctly than those based on intact cells. The results of the present study suggest that FTIR analysis of bacterial pigments is an easy and economical technique comparable to other phylogenetic markers, for the differentiation and characterization of bacteria.
- انتشار مقاله: 19-08-1395
- نویسندگان: Maryam Fanaei,Giti Emtiazi
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Basic Medical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: microscopy,16S rRNA Analysis Bacteriolytic activity Bdellovibrio Enterobacteriaceae Iran Isolation Predation Transmission electron
- چکیده:
- چکیده انگلیسی: Objective(s): Bdellovibrio-and-like organisms (BALOs) are predatory prokaryotes that attack and kill other Gram-negative bacteria for growth and reproduction. This study describes the isolation, identification, biological properties, and bacteriolytic activity of the first Bdellovibrio bacteriovorus with a broad prey range from Iran.
Materials and Methods: One BALO strain with high predatory potency was isolated from the rhizosphere soil using Enteropathogenic Escherichia coli as prey. It was identified and designated as Bdellovibrio bacteriovorus strain SOIR-1 through plaque assays, transmission electron microscopy (TEM), Bdellovibrio-specific PCRs, and 16S rRNA gene sequence analysis. Biological characterization and analysis of bacteriolytic activity were also performed.
Results: TEM and Bdellovibrio-specific PCRs confirmed that the strain SOIR-1 belongs to the genus Bdellovibrio. Analysis of the 16S rRNA gene sequence revealed its close phylogenetic relationship with strains of Bdellovibrio bacteriovorus. The strain SOIR-1 grew within the temperature range of 25–37 °C and the pH range of 6.0–8.0, with the optimal predatory activity at 30 °C and pH 7.4. It had the highest and lowest bacteriolytic activity toward Shigella dysenteriae and Pseudomonas aeruginosa with a killing rate of 89.66% and 74.83%, respectively.
Conclusion: Considering the hypothesis of bdellovibrios heterogeneity, identification of new isolates contributes to a deeper understanding of their diversity, their ecological roles, and their promising potential as living antibiotics or biocontrol agents. Bdellovibrios with broad bacteriolytic nature has not previously been reported in sufficient detail from Iran. The results of this study showed the great potential of native B. bacteriovorus strain SOIR-1 in the control and treatment of diseases caused by pathogenic Enterobacteriaceae.- انتشار مقاله: 24-06-1398
- نویسندگان: Salman Odooli,Rasoul Roghanian,Giti Emtiazi,Milad Mohkam,Younes Ghasemi
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Basic Medical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: microscopy,16S rRNA Analysis Bacteriolytic activity Bdellovibrio Enterobacteriaceae Iran Isolation Predation Transmission electron
- چکیده:
- چکیده انگلیسی: Objective(s): Bdellovibrio-and-like organisms (BALOs) are predatory prokaryotes that attack and kill other Gram-negative bacteria for growth and reproduction. This study describes the isolation, identification, biological properties, and bacteriolytic activity of the first Bdellovibrio bacteriovorus with a broad prey range from Iran.
Materials and Methods: One BALO strain with high predatory potency was isolated from the rhizosphere soil using Enteropathogenic Escherichia coli as prey. It was identified and designated as Bdellovibrio bacteriovorus strain SOIR-1 through plaque assays, transmission electron microscopy (TEM), Bdellovibrio-specific PCRs, and 16S rRNA gene sequence analysis. Biological characterization and analysis of bacteriolytic activity were also performed.
Results: TEM and Bdellovibrio-specific PCRs confirmed that the strain SOIR-1 belongs to the genus Bdellovibrio. Analysis of the 16S rRNA gene sequence revealed its close phylogenetic relationship with strains of Bdellovibrio bacteriovorus. The strain SOIR-1 grew within the temperature range of 25–37 °C and the pH range of 6.0–8.0, with the optimal predatory activity at 30 °C and pH 7.4. It had the highest and lowest bacteriolytic activity toward Shigella dysenteriae and Pseudomonas aeruginosa with a killing rate of 89.66% and 74.83%, respectively.
Conclusion: Considering the hypothesis of bdellovibrios heterogeneity, identification of new isolates contributes to a deeper understanding of their diversity, their ecological roles, and their promising potential as living antibiotics or biocontrol agents. Bdellovibrios with broad bacteriolytic nature has not previously been reported in sufficient detail from Iran. The results of this study showed the great potential of native B. bacteriovorus strain SOIR-1 in the control and treatment of diseases caused by pathogenic Enterobacteriaceae.- انتشار مقاله: 24-06-1398
- نویسندگان: Salman Odooli,Rasoul Roghanian,Giti Emtiazi,Milad Mohkam,Younes Ghasemi
- مشاهده
- جایگاه : پژوهشی
- مجله: Advanced Research in Microbial Metabolites & Technology
- نوع مقاله: Journal Article
- کلمات کلیدی: Nanoparticles,Bacterial Nano-Cellulose,Oleic acid,acetic and lactic acid
- چکیده:
- چکیده انگلیسی: Bacterial cellulose is a biological polymer with a variety of extraordinary properties which make it a functional material in different industrial fields. This work aimed at monitoring the effects of three different organic acids and nanoparticles on the production, water holding capacity and structural characteristics of bacterial cellulose. Different concentrations of organic acids and nanoparticles were used to detect their effect on cellulose synthesis, and the crystalline structure of produced bacterial cellulose was analyzed by FTIR. The results showed that acetic acid has the best effect on bacterial cellulose production with productivity of 1.23 g L-1 (1.8 – fold higher than control) followed by CuO nanoparticle, and lactic acid exhibits the minimum effect (0.74 g L-1). Oleic acid could improve bacterial cellulose production 1.5 fold higher than the control. From our FTIR results, the highest total crystalline index value (4.3) is related to the control bacterial cellulose representing the highest degree of crystallinity. Although acetic acid increases the production, but it has a negative effect on the total crystalline index values. The water holding capacity values of bacterial celluloses confirmed this assumption. Addition of CuO nanoparticle not only promotes production of cellulose but also does not change the crystallization so much compared to the control. Therefore we can use these data for improvement of bacterial cellulose production due to its great potential for biotechnological application.
- انتشار مقاله: 15-04-1396
- نویسندگان: Maryam Jalili Tabaii,Giti Emtiazi
- مشاهده