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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: Iranian Journal of Basic Medical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: Staphylococcus aureus,Pediatric,MRSA,Biofilm,Virulence factors,Fibronectin binding-proteins,MSCRAMMs
- چکیده:
- چکیده انگلیسی: Objective(s): Staphylococcus aureus can cause several infections. Its capability to form biofilm has been reported to be a vital property involved in the bacteria’s pathogenesis. Various genes contributing to biofilm formation have not yet been completely clarified. This study was designed to evaluate the factors influencing adherence and biofilm formation in S. aureus isolated from paediatric patients.
Materials and Methods: One hundred and ninety-seven S. aureus isolates were obtained from pediatric patients and confirmed with phenotypic and molecular examinations. Antimicrobial susceptibility testing and biofilm formation were evaluated using standard methods. The genes encoding adhesion and virulence factors were investigated by the PCR method.
Results: The most efficient antibiotics against S. aureus isolates were vancomycin and linezolid. Approximately, 54.2% of MSSA and 85.6% of MRSA isolates were biofilm producers according to the microtiter test. Our analysis indicated that MRSA isolates are better able to form biofilm compared with MSSA isolates. All isolates harbored clfA, fnbpA, icaA, icaB, icaC, and icaD, while clfB, fnbB, hlg, and pvl were detected in 99.5%, 42.1%, 97.5%, and 5.6% of isolates, respectively. In addition, a significant difference was found in fnhB gene and biofilm formation.
Conclusion: Our findings showed a significant correlation between mecA and pvl genes and MRSA and biofilm formation in S. aureus isolates. Additionally, this study indicated the significant role of the fnhB gene as a major marker for S. aureus biofilm formation. Therefore, further experiments are warranted to exactly elucidate the function of the fnhB gene in the formation of biofilm.- انتشار مقاله: 26-08-1397
- نویسندگان: Hiva Kadkhoda,Zohreh Ghalavand,Bahram Nikmanesh,Mansoor Kodori,Hamidreza Houri,Donya Taghizadeh Maleki,Ali Karimi Bavandpour,Gita Eslami
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Basic Medical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: Staphylococcus aureus,MRSA,Spa,SCCmec,agr,MLST
- چکیده:
- چکیده انگلیسی: Objective(s): In the current research, the prevalence of Staphylococcus aureus clones and genes encoding antimicrobial resistance and toxins were examined among 120 S. aureus strains from nosocomial infections in tehran, Iran.
Materials and Methods: Antimicrobial susceptibility was examined, based on disk diffusion and PCR method to identify resistance and toxin-encoding genes. Based on the polymorphisms in SCCmec, agr, spa, and MLST, the isolates were typed.
Results: Among 120 S. aureus isolates, 85 (70.8%) were methicilin resistant S. aureus (MRSA), and 35 (29.2%) were methicilin sensetive S. aureus (MSSA). The tested isolates contained resistance genes, including ant(4΄)-Ia (90%), aac(6΄)-Ie/aph(2˝) (80%), aph(3΄)-IIIa (30%), erm(A) (26.7%), erm(B) (10.8%), erm(C) (11.7%), msr(A) (40.8%), msr(B) (14.2%), tet(M) (45.8%), and mupA (8.3%). The MRSA strains were clustered into six different clones. The most common genotypes included ST239-SCCmec III/t037 (23.3%), ST239-SCCmec III/t388 (22.5%), ST22-SCCmec IV/t790 (8.3%), ST15-SCCmec IV/t084 (7.5%), ST585-SCCmec III/t713 (5%), and ST239-SCCmec III/t924 (4.2%), respectively. ST182/t196 (8.3%) and ST123/t171 (5%) belonged exclusively to MSSA strains. Overall, 10 (66.7%) and 5 (33.3%) out of 15 isolates with pvl genes were attributed to clones ST22-SCCmec IV/t790 and ST15-SCCmec IV/t084, respectively. ST22-SCCmec IV/t790, ST239-SCCmec III/t037, and ST15-SCCmec IV/t084, were related to high-level mupirocin-resistant phenotypes.
Conclusion: The genetic diversity of S. aureus was confirmed in our hospitals, and ST239-SCCmec III/t037 showed a relatively high prevalence in our study. It seems that assessment of resistance and virulence genes in different S. aureus molecular types is necessary for proper antibiotic consumption.- انتشار مقاله: 03-12-1396
- نویسندگان: Mehdi Goudarzi,Gita Eslami,Razieh Rezaee,Mohsen Heidary,Saeed Khoshnood,Raheleh Sajadi Nia
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Basic Medical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: Staphylococcus aureus,Pediatric,MRSA,Biofilm,Virulence factors,Fibronectin binding-proteins,MSCRAMMs
- چکیده:
- چکیده انگلیسی: Objective(s): Staphylococcus aureus can cause several infections. Its capability to form biofilm has been reported to be a vital property involved in the bacteria’s pathogenesis. Various genes contributing to biofilm formation have not yet been completely clarified. This study was designed to evaluate the factors influencing adherence and biofilm formation in S. aureus isolated from paediatric patients.
Materials and Methods: One hundred and ninety-seven S. aureus isolates were obtained from pediatric patients and confirmed with phenotypic and molecular examinations. Antimicrobial susceptibility testing and biofilm formation were evaluated using standard methods. The genes encoding adhesion and virulence factors were investigated by the PCR method.
Results: The most efficient antibiotics against S. aureus isolates were vancomycin and linezolid. Approximately, 54.2% of MSSA and 85.6% of MRSA isolates were biofilm producers according to the microtiter test. Our analysis indicated that MRSA isolates are better able to form biofilm compared with MSSA isolates. All isolates harbored clfA, fnbpA, icaA, icaB, icaC, and icaD, while clfB, fnbB, hlg, and pvl were detected in 99.5%, 42.1%, 97.5%, and 5.6% of isolates, respectively. In addition, a significant difference was found in fnhB gene and biofilm formation.
Conclusion: Our findings showed a significant correlation between mecA and pvl genes and MRSA and biofilm formation in S. aureus isolates. Additionally, this study indicated the significant role of the fnhB gene as a major marker for S. aureus biofilm formation. Therefore, further experiments are warranted to exactly elucidate the function of the fnhB gene in the formation of biofilm.- انتشار مقاله: 26-08-1397
- نویسندگان: Hiva Kadkhoda,Zohreh Ghalavand,Bahram Nikmanesh,Mansoor Kodori,Hamidreza Houri,Donya Taghizadeh Maleki,Ali Karimi Bavandpour,Gita Eslami
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Basic Medical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: Staphylococcus aureus,MRSA,Spa,SCCmec,agr,MLST
- چکیده:
- چکیده انگلیسی: Objective(s): In the current research, the prevalence of Staphylococcus aureus clones and genes encoding antimicrobial resistance and toxins were examined among 120 S. aureus strains from nosocomial infections in tehran, Iran.
Materials and Methods: Antimicrobial susceptibility was examined, based on disk diffusion and PCR method to identify resistance and toxin-encoding genes. Based on the polymorphisms in SCCmec, agr, spa, and MLST, the isolates were typed.
Results: Among 120 S. aureus isolates, 85 (70.8%) were methicilin resistant S. aureus (MRSA), and 35 (29.2%) were methicilin sensetive S. aureus (MSSA). The tested isolates contained resistance genes, including ant(4΄)-Ia (90%), aac(6΄)-Ie/aph(2˝) (80%), aph(3΄)-IIIa (30%), erm(A) (26.7%), erm(B) (10.8%), erm(C) (11.7%), msr(A) (40.8%), msr(B) (14.2%), tet(M) (45.8%), and mupA (8.3%). The MRSA strains were clustered into six different clones. The most common genotypes included ST239-SCCmec III/t037 (23.3%), ST239-SCCmec III/t388 (22.5%), ST22-SCCmec IV/t790 (8.3%), ST15-SCCmec IV/t084 (7.5%), ST585-SCCmec III/t713 (5%), and ST239-SCCmec III/t924 (4.2%), respectively. ST182/t196 (8.3%) and ST123/t171 (5%) belonged exclusively to MSSA strains. Overall, 10 (66.7%) and 5 (33.3%) out of 15 isolates with pvl genes were attributed to clones ST22-SCCmec IV/t790 and ST15-SCCmec IV/t084, respectively. ST22-SCCmec IV/t790, ST239-SCCmec III/t037, and ST15-SCCmec IV/t084, were related to high-level mupirocin-resistant phenotypes.
Conclusion: The genetic diversity of S. aureus was confirmed in our hospitals, and ST239-SCCmec III/t037 showed a relatively high prevalence in our study. It seems that assessment of resistance and virulence genes in different S. aureus molecular types is necessary for proper antibiotic consumption.- انتشار مقاله: 03-12-1396
- نویسندگان: Mehdi Goudarzi,Gita Eslami,Razieh Rezaee,Mohsen Heidary,Saeed Khoshnood,Raheleh Sajadi Nia
- مشاهده
- جایگاه : پژوهشی
- مجله: International Journal of Molecular and Clinical Microbiology
- نوع مقاله: Journal Article
- کلمات کلیدی: Infection,Antibiotic Susceptibility,Extract,green tea,Vaginitis
- چکیده:
- چکیده انگلیسی: Introduction: Vaginitis is an inflammatory and infectious disease in human host that involved vaginal mucosa. Increase of resistance to various antibacterial agent has been very significant in three or four recent decades and this important subject reveals the use of new and natural sources such as plants with medical and antimicrobial property for eradication of these infections. Therefore, the aim of this study is to evaluate the antibacterial effect of green tea plant on vaginitis agents.
Materials and Methods: This experimental study was performed on 60 samples obtained from patients with genital infection. Then, samples were introduced to transport medium and transported to microbiology laboratory of Shahid Beheshti University of Medical Science. Incubation was performed after culturing the samples in specific medium. Microorganisms were identified responsible for vaginitis and isolated after growth of colonies and performing the confirmation tests. Finally, disk diffusion and MIC tests were performed for detection of susceptibility and resistance of infections to 6 mentioned antibiotics in comparison with green tea extract.
Risults: The antibacterial effect of green tea extract on lactobacillus and listeria was more effective in comparison with current antibiotics except ciprofloxacin. Green tea extract had antibacterial effect on B group streptococcus as like as gentamicin and ampicillin.
Conclusion: Green tea extract can be used as an effective antimicrobial agent in conjunction with other antibiotics for the treatment of vaginal infections in women.- انتشار مقاله: 22-07-1394
- نویسندگان: Masoud Dadashi,Mina Amjadi Kashani,Gita Eslami,Hossein Goudarzi,Fatemeh Fallah,Parviz Owlia,Nafiseh Adhami,Tahoura Mousavi
- مشاهده
- جایگاه : پژوهشی
- مجله: International Journal of Molecular and Clinical Microbiology
- نوع مقاله: Journal Article
- کلمات کلیدی: Antibacterial effect,urinary tract infection,Extract,Cinnamon,antibiotic sensitivity
- چکیده:
- چکیده انگلیسی: With using antibiotic in societies which usage of this kind of the drug without any limitation cause appear resistance against antibiotics.This survey consider to use the extract and essence of the cinnamon (which have a so many rate of planting in Iran) and also survey on extract on bacteria whose cause urinary tract infections, and compare this with common antibiotics. This study was experimental design.We have been isolate the E.coli,Klebsiella pneumoniae, Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus agalactiae and Enterococcus faecalis from UTI and then determine of antibacterial effect of cinnamon against this bacteria with subculture and put the exact diagnosis on them. Antibacterial effects of the herb extract, nalidixic acid and Co-trimoxazol were evaluated by method of agar disc diffusion.Enterococcus faecalis, E.coli and Streptococcus agalactiae had 100% sensitivity in extract and essence. Staphylococcus epidermidis and MRSA had 80% sensitivity to extract and essence,and Klebsiella pneumonie had 90% sensitivity to extract and essence of cinnamon. After all , we totally conclude that essence and extract of cinnamon have a much more effectiveness on bacteria isolated from UTI.
- انتشار مقاله: 14-10-1393
- نویسندگان: Masoud Dadashi,Gita Eslami,Hossein Goudarzi,Fatemeh Fallah,Hossein Dabiri,Ali Hashemi,Najmeh Ardeshiri,Mohammad Javad Nasiri
- مشاهده
- جایگاه : پژوهشی
- مجله: Avicenna Journal of Phytomedicine
- نوع مقاله: Journal Article
- کلمات کلیدی: Capsaicin,Vibrio cholera,Antivirulence agents,Toxin gene expression
- چکیده:
- چکیده انگلیسی: Objective: Cholera is an acute secretory diarrhea caused by the Gram-negative bacterium, Vibrio cholerae mostly through production of cholera toxin (CT) and zonula occludens toxin (Zot). Isolates of V. cholerae have acquired resistance elements during the last decade. One of the most promising ways to treat resistant strains is to use antivirulence agents instead of killing the causative agent with conventional antibiotics. In this study, we examined whether different concentrations of capsaicin - the pungent fraction of red chili- can act as an antivirulence agent and inhibit V. cholerae toxin production.
Materials and Methods: Two standard strains namely, V. cholerae ATCC 14035 and V. cholerae PTCC 1611 were used in this study. Minimum Inhibitory Concentration (MIC) of capsaicin was determined by broth microdilution method. Based on MIC results, the bacteria were cultured in the presence of sub-MIC concentrations of capsaicin and a negative control without capsaicin. Real-time PCR (RT-PCR) was carried out to determine the expression level of V. cholerae toxin genes at each concentration.
Results: MIC test showed that 200 mg/mL of capsaicin in 2% dimethyl sulfoxide (DMSO) could inhibit the growth of the two standard strains of V. cholerae. The expression of V. cholerae toxin genes was significantly reduced following treatment with sub-MIC concentrations of capsaicin as assessed by RT-PCR.
Conclusion: Capsaicin showed great inhibitory effect against cholera toxin and reduced Zot production in the tested strains of V. cholerae. The results showed promising insights into antivirulence effects of capsaicin.- انتشار مقاله: 25-03-1396
- نویسندگان: Soroor Erfanimanesh,Gita Eslami,Arezou Taherpour,Ali Hashemi
- مشاهده
- جایگاه : پژوهشی
- مجله: Avicenna Journal of Phytomedicine
- نوع مقاله: Journal Article
- کلمات کلیدی: Klebsiella pneumoniae,Antibiotic resistance,Zataria multiflora,Extended-Spectrum-β-Lactamases (ESBLs)
- چکیده:
- چکیده انگلیسی: Objective: There are few therapeutic options for treatment of multidrug resistant Klebsiella pneumoniae isolates as a hospital infectious agent (nosocomial infection). The aim of this study was to evaluate the antibacterial activity of Zataria multiflora Boiss extracts against ESBL-producing Klebsiella pneumoniae strains.
Materials and Methods: This study was conducted on 100 K. pneumoniae isolates from two hospitals in Tehran, Iran. Antibiotic susceptibility tests were performed by Kirby-Bauer disc diffusion and microdilution broth methods and detection of ESBL was carried out according to CLSI guidelines. The blaCTX-M-15plasmid genewas detected by PCR and sequencing methods. Extracts susceptibility test was performed by broth microdilution method.
Results: Among 100 K. pneumoniae strains, 48 (48%) were ESBL positive. In this study, fosfomycin, colistin and tigecycline were more active than other antibiotics. The existence of blaCTX-M-15 was detected in 30 (62.5%) of 48 ESBL-producing isolates. The chloroformic extract showed potent activity against ESBL-producing K. pneumoniae strains (MIC50 = 1.56 mg/ml and MIC90=3.12mg/ml). The MIC50 and MIC90 (The MIC50 represents the MIC value at which ≥50% of the isolates in a test population are inhibited and the MIC90 represents the MIC value at which ≥90% of the strains within a test population are inhibited) were 3.12 and 6.25 mg/ml and 6.25 and 12.5 mg/ml for methanolic and acetonic extracts, respectively.
Conclusions: The incidence of ESBL-producing K. pneumoniae is very high. Therefore, detection of ESBL-producing K. pneumoniae isolates is of great importance in identifying drug resistance patterns in K. pneumoniae isolates and in control of infections. Zataria multiflora may have the potential to be used against multidrug resistant organisms such as clinical isolates of ESBL-producing K. pneumoniae.- انتشار مقاله: 06-02-1394
- نویسندگان: Masoud Dadashi,Ali Hashemi,Gita Eslami,Fatemeh Fallah,Hossein Goudarzi,Soroor Erfanimanesh,Arezou Taherpour
- مشاهده
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: HPV,E-cadherin,LPS,Snail,ZEB-1
- چکیده:
- چکیده انگلیسی: Objective: Cancer is one of the common diseases in the world, and cervical cancer is the fourth one. In this type of cancer, many risk factors, especially infectious diseases, such as human papilloma virus (HPV) and gram-negative bacteria can have important effects on the expression of epithelial to mesenchymal transition related genes like Snail, E-cadherin, and ZEB-1, responsible for connecting cell tissues. In this study, we have investigated the effect of Escherichia coli O111:B4 Lipopolysaccharide (LPS) on HPV positive cell line (HeLa), the expression level of the (Snail, E-cadherin, and ZEB-1), HPV oncogenes (E6, E7) and also microRNA-9, 192. Materials and Methods: HeLa cell line was treated with LPS to analyze Snail, E-cadherin, ZEB-1, E6, E7 and also microRNA-9, 192 expression by quantitative real-time PCR in 24, 48 and 72 hours. Results: Quantitative real-time PCR revealed a significant reduction in E-cadherin mRNA level at 10ug/L of LPS in three time-points and after 24 hours at 5ug/L of LPS; however, ZEB-1 at 10ug/L of LPS and Snail at 5, 10ug/L of LPS are up-regulated. E7 also illustrated a slight increase, but we did not find any relationship between E7 and LPS treatment. Additionally, there are upward trends in microRNA-9, 192 levels. Conclusion: The result of this study, LPS is able to reduce E-cadherin expression, caused by increase in repressor E-cadherin protein expression and some microRNAs, probably. Since bacterial infection can be in cervical site, it is likely to be effective in reducing the E-cadherin expression in the EMT and enhance cancer process, therefore; removing these infections by using the appropriate antibiotics may result in slowing down this process, which requires more research.
- انتشار مقاله: 28-02-1398
- نویسندگان: Shaian Tavakolian,Hossein Goudarzi,Gita Eslami,Ebrahim Faghihloo
- مشاهده