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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: Research Journal of Pharmacognosy
- نوع مقاله: Journal Article
- کلمات کلیدی: Antioxidants,Malondialdehyde,Injury,Pistacia,Vascular endothelial growth factor
- چکیده:
- چکیده انگلیسی: Background and objectives: Severe burn damage and its consequences are life threatening which can complicate patients’ health. Burn damage increases reactive oxygen species (ROS) generation which leads to severe damage to tissues and is implicated in burn shock. Some medicinal and traditional plants are considered as safe, natural and inexpensive sources for treatment of different of diseases. Pistacia atlantica has shown anti-inflammatory and anti-oxidant properties and has also been used traditionally as an ointment for wound healing in some parts of Iran. In this study, the beneficial effect of P. atlantica resin oil on rats’ burn wound healing and its potential effects on vascular endothelial growth factor (VEGF), hydroxyproline and antioxidants in wound area was examined. Methods: Thirty male rats (200±10 g) were randomly and divided into three groups (n=10) as follow: Group 1: burn injury, Group 2: burn injury receiving 300 μL/kg/day P. atlantica resin oil topically, Group 3: burn injury receiving 300 mg/kg/day sulfadiazine cream topically. At the end of the study (day 14) the area of wounds were measured and then skins with burn damage were dissected and anti-oxidative parameters, VEGF and hydroxyproline were evaluated. Results: We found that Pistacia Atlantica oil significantly increased antioxidant defense, VEGF and hydroxyproline and reduced malondialdehyde (MDA) levels. Pistacia atlantica remarkably reduced wound size compared to burn the control group and showed more beneficial effects compared to sulfadiazine as the positive control. Conclusion: Pistacia atlantica resin oil could be considered as a new therapeutic agent for treatment of injuries such as burn damages.
- انتشار مقاله: 17-01-1397
- نویسندگان: Beydolah Shahouzehi*,Gholamreza Sepehri,Sakine Sadeghiyan,Yaser Masoumi-Ardakani
- مشاهده
- جایگاه : پژوهشی
- مجله: Journal of Kerman University of Medical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: Rat,Morphine,Nicotine,Cytokine,Marijuana use
- چکیده:
- چکیده انگلیسی: Background: Simultaneous co-consumption of abused substances is common among drug users. This study examined the effects of co-administration of marijuana/ nicotine and morphine on some cytokines in rats.
Method: Ninety eight rats were randomly divided into fourteen experimental groups including control (saline 1 ml/kg, i.p.), morphine (1, 3 and 5 mg/kg, i.p.), nicotine (0.5, 2 and 4 mg/kg, i.p.), marijuana (2.5, 5 and 10 mg/kg, i.p.) and the combination groups in which the rats received the combination of either effective or sub-effective doses of nicotine/ marijuana and morphine. Inflammation was induced via formalin injection into the left hind paw of all the control and the treated rats. The serum concentrations of some cytokines (TNF-ɑ, IL-1, and IL-6) were measured by using an enzyme-linked immune-sorbent assay (ELISA) technique.
Results: A significant reduction in TNF-ɑ, IL-1, and IL-6 concentration was observed in marijuana, nicotine and morphine treated rats. Also, the co-administration of effective doses of marijuana/ nicotine and morphine caused a significant reduction in cytokines, indicating either an additive or a synergistic effect.
Conclusion: The clinical application for the combined use of these substances has not been determined yet and further research is needed to clarify the efficacy, safety and tolerability of these combinations in inflammatory process.- انتشار مقاله: 21-12-1398
- نویسندگان: Gholamreza Sepehri,Mehrnoush Ranjbar,Manzumeh Shamsi Meymandi,Sara Dahesh
- مشاهده
- جایگاه : پژوهشی
- مجله: Journal of Kerman University of Medical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: Iran,Kerman province,Outpatients,Medicine prescription,Orthopedists
- چکیده:
- چکیده انگلیسی: Background: This study was aimed to evaluate the orthopedists’ prescription pattern for outpatients referred to private clinics in Kerman province, Iran. Methods: In this study a total of 59,613 outpatients’ prescriptions issued by orthopedists contracting with Iran Health insurance organization and Iran Social Security Organization from the 1st May 2014 to the 1st May 2015 were analyzed for mean number of drugs per prescription, route of administration, drug category, drug names and the most common prescribed drugs. Results: Mean number of drugs per prescription was 2.48. Non-steroidal anti-inflammatory drugs (NSAIDs) were the most frequently prescribed drugs (60.34%) followed by vitamins & minerals (53.88%), skeletal muscle relaxants (33.18%) and corticosteroids (33.0 %). Naproxen 250mg (14.29%) and diclofenac 100mg (7.5%) were the most frequently prescribed NSAIDs. Vitamin D3 (injection & oral soft gelatin capsule) was the most frequently prescribed vitamin & mineral (24.72%). Gabapentin 300mg (12.73%) and methocarbamol 500 mg (10.59%) were the most frequently prescribed skeletal muscle relaxants. Methylprednisolone acetate (10.48%) and triamcinolone acetonide (7.45%) were the most frequently prescribed corticosteroids. Anti-ulcer drugs were prescribed only for 4.11% of outpatients. Conclusion: NSAIDs, vitamins & minerals and corticosteroids were the most frequent prescribed drugs by orthopedists in Iran. The high rate of vitamin D3 and calcium prescription is a valuable finding for the prevention and treatment of osteoporosis; however, anti-ulcer drugs were prescribed lower than the requiered rate and this was a remarkable finding which increases the risk of NSAIDs /corticosteroids- induced gastropathies. Therefore, some interventions for improving drug prescription by orthopedists are suggested.
- انتشار مقاله: 30-04-1398
- نویسندگان: Gholamreza Sepehri,Zohreh Ebrahimi-Meimand
- مشاهده
- جایگاه : پژوهشی
- مجله: Journal of Kerman University of Medical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: Rat,diabetes,Prosopis Farcta,Blood glucose,Streptozocin
- چکیده:
- چکیده انگلیسی: Background: Previous studies have shown the beneficial effects of Prosopis species in the treatment of diabetes in traditional medicine. This study was performed to evaluate the antihyperglycemic effects of Prosopis farcta (P.farcta) in streptozocin- induced diabetic rats.
Methods: Diabetes was induced by intraperitoneal injection of streptozocin (55mg/kg). Male Wistar rats were treated with either P. farcta (100, 150, and 300 mg/kg.) or glibenclamide (10mg/kg) orally once a day for a period of 28 days. Control rats received saline. Changes in body weight and blood glucose were measured at the end of each week for 4 weeks.
Results: The results of this study showed a significant increase in blood glucose, and decrease of body weight in streptozocin-induced diabetic rats. P. farcta administration for 28 days in streptozocin-induced diabetic rats suppressed the weight reduction significantly in a dose dependent manner (P<0.001). Also, P. farcta, like glibenclamide, showed significant antihyperglycemic effects and reversed the above parameters significantly in a dose dependent manner when compared to diabetic control rats (P<0.001, P<0.05, respectively).
Conclusions: The results of the present study showed that P. farcta possesses antidiabetic activity in hyperglycemic rat models. The underlying mechanism(s) has not been known yet and needs further investigation.- انتشار مقاله: 25-08-1395
- نویسندگان: Sahel Heidar Lashkari Heidar Lashkari,Gholamreza Sepehri,Ladan Emadi,Sahel Motaghi
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Medical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: Rats,Folic acid,Cotrimoxazole,Ovarian maturation status,Ovarian histopathology
- چکیده:
- چکیده انگلیسی: Background: Previous studies have reported the antifertility activities of sulfonamides. This study was designed to evaluate the effects of co-trimoxazole and its co-administration with folic acid on ovarian tissue in female rats.Methods: A total of 54 rats were randomly divided into 9 groups (n=6). Group I served as the control and group II (vehicle) received saline. Other groups, III to IX, received co-trimoxazole (30, 60, and 120 mg/kg; i.p.), folic acid (1 mg/kg; i.p.) or their combination for 14 days, respectively. The oocytes were obtained from each group at the end of the 14th days and scored for maturational status as germinal vesicle (GV), metaphase I (MI), or metaphase II (MII). The number of primordial follicle (PrF), primary follicle (PF), and secondary follicle in formalin-fixed ovaries were counted under light microscopy. The data were analyzed by one-way ANOVA followed by post-hoc Dunnet test using SPSS statistical software (version 17.0). Results were considered statistically significant at P<0.05.Results: Co-trimoxazole (60 and 120 mg/kg) treatment for 14 days caused a significant decrease in the number of GV (P=0.02, P<0.001), MI and MII (P=0.03, P<0.001), a significant increase in structural abnormalities, including PrF, PF and secondary follicle (P<0.001) as well as congestion, inflammation and necrosis of ovarian tissue compared to the vehicle group. Folic acid co-administration with co-trimoxazole reversed partially all these parameters compared to the co-trimoxazole group (P<0.001).Conclusion: The data showed the adverse effects of co-trimoxazole on the ovarian maturational status and tissue structure which was reversed partially by folic acid co-administration in rats.
- انتشار مقاله: 04-05-1395
- نویسندگان: Arezoo Saberi,Ehsan Salarkia,Zohreh Safi,Gholamreza Sepehri
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Basic Medical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: Opium,Morphine,Learning,Morris Water Maze,Co-administration,Nicotine dependency
- چکیده:
- چکیده انگلیسی: Objective(s): Smoking opium/cigarette is a global health concern. The aim of this study was to examine learning and memory of rat male offsprings whose mothers had been exposed to either opium or morphine with nicotine during pregnancy. Materials and Methods: Wistar rats were used for the experiments. In the female rats, opium, morphine and nicotine dependencies were induced by daily injections of drug solution for 10 days before mating. Spatial memory was tested by Morris water maze test in male pups at the postnatal day 60. The duration that took until the rats found the platform in the maze and also their swimming speed were recorded. Results: An increase in the platform finding duration was observed for the pups of dependent mothers in comparison with the control in the training trial (P<0.05). Prenatal exposure to opium/morphine and nicotine significantly decreased the time spent in the trigger zone to find the hidden platform (P<0.05) but had no significant effect on the swimming speed in the probe test. However, no significant difference was observed in the learning and memory behavior of offspring whose mothers received morphine, opium, nicotine or the co-administration of either morphine or opium with nicotine. Conclusion: The present study showed that the opium, morphine and nicotine abuse and co-administration of opium/morphine with nicotine during pregnancy may cause deficits in spatial learning of male rat offspring. Based on our data, no synergistic effects of co-drug administration were observed on learning and memory in male rat offspring.
- انتشار مقاله: 02-07-1393
- نویسندگان: Gholamreza Sepehri,Shahrnaz Parsania,Mousa‐Al‐Reza Hajzadeh,Tahereh Haghpanah,Vahid Sheibani,Kouros Divsalar,Shahnaz Shekarforoush,Mohammad Reza Afarinesh
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Basic Medical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: Medicinal plant,Spatial learning,Origanum vulgare,Antioxidant assay,Long term potentiation,T-maze
- چکیده:
- چکیده انگلیسی: Objective(s)
The objective of this study was to determine the effect of aqueous extract of Origanum vulgare L. ssp. Viridis (ORG) on discrimination learning and long term potentiation (LTP) in CA1 region of the rat hippocampus.
Materials and Methods
A group of adult male Wistar rats weighing 275±25 g received aqueous extract of ORG (150, 300, 450 mg/kg/day) by intraperitoneal injection for one week, and the other group received saline (n= 6). A wooden T-maze was used to evaluate the discrimination learning. In electrophysiological experiments, the effect of ORG leaves extract on induction and maintenance of long term potentiation (LTP) in CA1 hippocampus area was determined. LTP was evaluated in CA1 region after high-frequency stimulation (200 Hz) of the Schaffer collaterals. Also, serum antioxidant levels were analyzed in the two groups (n= 4).
Results
Statistical analysis showed significant decreases in the number of total (significantly at the dose of 300 and 450 mg/kg) and wrong (significantly at the dose of 300 mg/kg) entrance into opposite box of T-maze procedure in ORG-treated animals (P< 0.05). In electrophysiological study, the rats which had received ORG (150, 300, and 450 mg/kg) showed an increase in both population spike amplitude (59.7±14.1%, 85±14.7% and 49.3±8.7% respectively, compared to 39±9.2% increase in saline group) and maintenance of LTP in hippocampus CA1 after high frequency stimulation in Schaffer collateral pathway. In serum antioxidant assay, level of antioxidants in ORG groups (300 and 450 mg/kg) remarkably increased in comparison to saline group (P< 0.05 and P< 0.001, in turn).
Conclusion
Our results suggest that Origanum aqueous extract can improve the learning criteria in rats.- انتشار مقاله: 30-06-1394
- نویسندگان: Vahid Sheibani,Mohammadreza Afarinesh,Zahra Hajializadeh,Mehdi Abbasnejad,Tahereh Haghpanah,Razieh Arabnezhad,Gholamreza Sepehri
- مشاهده
- جایگاه : پژوهشی
- مجله: Addiction and Health
- نوع مقاله: Journal Article
- کلمات کلیدی: Methamphetamine,Rats,histology,spermatogenesis,Testis
- چکیده:
- چکیده انگلیسی: Background: Methamphetamine (mAMP) as a recreational drug has devastating effects on the central nervous system (CNS). Several studies have shown that mAMP has inhibitory effects on oogenesis and spermatogenesis, and causes impaired fertility. This study designed to investigate the effect of mAM Padministration on histological changes and spermatogenesis indices in the testis of adult male rats.Methods: In this experimental study, 50 male Wistar rats were randomly divided into control (received no treatment, n = 10), vehicle (received saline for 7 and 14 days, n = 20), and experimental group [received mAMP, 5 ml/kg, intraperitoneal (IP) for 7 and 14 days, n = 20]. Testicular tissue samples were stained by hematoxylin and eosin (H&E) technique. For histological study, we counted the number of spermatogonia, spermatocytes and Leydig cells. Spermatogenesis indices which include: tubular differentiation index (TDI), spermiogenesis index (SI), repopulation index (RI) and the mean seminiferous tubules diameter (MSTD) were studied. Data were analyzed by one-way ANOVA, using SPSS software. P < 0.05 was considered statistically significant.Findings: This study showed that mAMP caused a significant decrease in number of seminiferous tubules cells and spermatogenesis in treated group compared with the control group. Moreover, results showed a significant decrease in spermatogenesis indices including TDI, SI, RI, and MSTD in 14th day, compared to control group (P < 0.001).Conclusion: The data showed the adverse effects of mAMP administration (for 7 and 14 days) on testes structure and spermatogenesis indices in rat testis tissue. The underlying mechanism(s) needs further investigation.
- انتشار مقاله: 28-11-1396
- نویسندگان: Arezoo Saberi,Gholamreza Sepehri,Zohreh Safi,Behzad Razavi,Faranak Jahandari,Kouros Divsalar,Ehsan Salarkia
- مشاهده
- جایگاه : پژوهشی
- مجله: Addiction and Health
- نوع مقاله: Journal Article
- کلمات کلیدی: Methamphetamine,Neurodegeneration,cerebellum,CB1 receptor
- چکیده:
- چکیده انگلیسی: Background: A number of neuroimaging studies on human addicts have revealed that abuse of Methamphetamine (METH) can induce neurodegenerative changes in various brain regions like the cerebral cortex and cerebellum. Although the underlying mechanisms of METH-induced neurotoxicity have been studied, the cellular and molecular mechanisms of METH-induced neurotoxicity remain to be clarified. Previous studies implicated that cannabinoid type 1 receptors (CB1Rs) exert neuroprotective effects on several models of cerebral toxicity, but their role in METH-induced neurotoxicity has been rarely investigated. Moreover, the cerebellum was considered as a potential target to evaluate the effects of cannabinoids on locomotion activity as the CB1Rs are most widely distributed in the molecular layer of cerebellum. Therefore, the present study was carried out to evaluate whether neurodegeneration induced in the cerebellum tissue implicated in locomotion deficit induced by METH.Methods: In the current study, open field test was used to examine locomotor activity. Using hematoxylin and eosin (H&E) staining, morphology of the cerebellar vermis was investigated after repeated exposure to METH. Then, the effects of CB1Rs antagonist [SR17141A, 10 mg/kg, intraperitoneally (IP)] and CB1Rs agonist [WIN55, 212-2 (WIN), 3 mg/kg] against METH-induced neurodegeneration and locomotor deficit were assessed.Findings: The results of the present study demonstrated that repeated exposure to METH increased cerebellar degeneration level as compared to the saline and dimethyl sulfoxide (DMSO) groups. In addition, METH-treated rats showed hyperactivity as compared to the saline and DMSO groups. Pretreatment with WIN significantly attenuated neurodegeneration and hyperactivity induced by METH.Conclusion: The findings of this study provided evidence that CB1Rs may serve as a therapeutic strategy for attenuation of METH-induced locomotor deficits.
- انتشار مقاله: 07-10-1396
- نویسندگان: Effat Ramshini,Shahriar Dabiri,Shokouh Arjmand,Gholamreza Sepehri,Mohammad Khaksari,Meysam Ahmadi,Mohammad Shabani
- مشاهده
- جایگاه : پژوهشی
- مجله: Addiction and Health
- نوع مقاله: Journal Article
- کلمات کلیدی:
- چکیده:
- چکیده انگلیسی: Abstract Background: Athletes, especially bodybuilders, abuse anabolic steroid drugs to improve their strength and enhance their muscle growth and appearance. This study was conducted to determine the type and frequency of anabolic steroids abuse in bodybuilder athletes in Kerman City. Methods: A confidential questionnaire which included demographic data (age, education), name of abused anabolic drug and duration of drug abuse was completed by 202 bodybuilder athletes, and the collected data were analyzed using Chi Square test. A value of p < 0.05 was considered significant. Findings: The frequency of anabolic steroid abuse was 18.8%. The mean period of bodybuilding activity was significantly higher in those used the anabolic drugs (38.8 months), comparing to those did not use any drugs (14.3 months). Oxymetholone was the most common drug used by athletes (42% merely used Oxymetholone). The frequency of anabolic steroids abuse was not related to education and age of the bodybuilder athletes. Conclusion: Bodybuilder athletes in Kerman city abuse anabolic steroids, and the health care system should plan to inform them about anabolic steroid adverse effects. Keywords: Anabolic steroid, Bodybuilder athletes, Drug abuse
- انتشار مقاله: 17-08-1389
- نویسندگان: Gholamreza Sepehri,Majid Mousavi Fard,Ehsan Sepehri
- مشاهده