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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: Nanomedicine Journal
- نوع مقاله: Journal Article
- کلمات کلیدی: Rat,Gold nanoparticles,Nebulization,Postoperative peritoneal adhesion
- چکیده:
- چکیده انگلیسی: Objective(s):
Abdominal adhesions are one of the most important problems, occurring after intra-abdominal surgery in more than 90% of cases. This condition is the leading cause of bowel obstruction, infertility, and abdominal/pelvic pain. Gold nanoparticles (GNPs) have been shown to be non-toxic and exhibit anti-inflammatory, anti-angiogenic and antioxidant activities. The purpose of this study was to determine the effect of intraperitoneal lavage with GNP solutions on the development of postoperative peritoneal adhesion (PPA).
Materials and Methods:
In the current experimental study, thirty-five male Wistar rats were randomly assigned to seven groups of five rats. After a standardized peritoneal injury, GNP solutions in different concentrations (1, 2.5, 5, 10, 50 and 100 ng/ml) were locally administered through nebulization; normal saline (NS) was administered to the control group. Two weeks later, the rats were sacrificed and cecum and peritoneal samples were harvested for histopathological assessment. Blood samples were obtained to determine serum concentrations of inflammatory biomarkers including tumor necrosis factor alpha (TNF-α), interleukin-1 beta (IL-1β) and vascular endothelial growth factor (VEGF).
Results:
The rats treated with GNPs had significantly lower microscopic and macroscopic peritoneal adhesion scores, compared to the control group (P<0.05). Score 5 of macroscopic adhesions was reported in all the rats of the control group, unlike the GNP groups. Furthermore, microscopic adhesions were reported with all rats in the control group, unlike the GNP groups (reported in 0 out of 5 rats in all GNP groups). In addition, serum levels of IL-1β, TNF-α and VEGF underwent no significant changes.
Conclusion:
Compared to the control group, GNPs decreased the severity of peritoneal adhesions, although they did not alter TNF-α, IL-1β or VEGF serum levels.- انتشار مقاله: 31-03-1394
- نویسندگان: Amir Hooshang Mohammadpour,Alireza Tavassoli,Mohammad Reza Khakzad,Elahe Zibaee,Mohammad Afshar,Mahmoud Hashemzaei,Gholamreza Karimi
- مشاهده
- جایگاه : پژوهشی
- مجله: Nanomedicine Journal
- نوع مقاله: Journal Article
- کلمات کلیدی: Rat,Gold nanoparticles,Nebulization,Postoperative peritoneal adhesion
- چکیده:
- چکیده انگلیسی: Objective(s):
Abdominal adhesions are one of the most important problems, occurring after intra-abdominal surgery in more than 90% of cases. This condition is the leading cause of bowel obstruction, infertility, and abdominal/pelvic pain. Gold nanoparticles (GNPs) have been shown to be non-toxic and exhibit anti-inflammatory, anti-angiogenic and antioxidant activities. The purpose of this study was to determine the effect of intraperitoneal lavage with GNP solutions on the development of postoperative peritoneal adhesion (PPA).
Materials and Methods:
In the current experimental study, thirty-five male Wistar rats were randomly assigned to seven groups of five rats. After a standardized peritoneal injury, GNP solutions in different concentrations (1, 2.5, 5, 10, 50 and 100 ng/ml) were locally administered through nebulization; normal saline (NS) was administered to the control group. Two weeks later, the rats were sacrificed and cecum and peritoneal samples were harvested for histopathological assessment. Blood samples were obtained to determine serum concentrations of inflammatory biomarkers including tumor necrosis factor alpha (TNF-α), interleukin-1 beta (IL-1β) and vascular endothelial growth factor (VEGF).
Results:
The rats treated with GNPs had significantly lower microscopic and macroscopic peritoneal adhesion scores, compared to the control group (P<0.05). Score 5 of macroscopic adhesions was reported in all the rats of the control group, unlike the GNP groups. Furthermore, microscopic adhesions were reported with all rats in the control group, unlike the GNP groups (reported in 0 out of 5 rats in all GNP groups). In addition, serum levels of IL-1β, TNF-α and VEGF underwent no significant changes.
Conclusion:
Compared to the control group, GNPs decreased the severity of peritoneal adhesions, although they did not alter TNF-α, IL-1β or VEGF serum levels.- انتشار مقاله: 31-03-1394
- نویسندگان: Amir Hooshang Mohammadpour,Alireza Tavassoli,Mohammad Reza Khakzad,Elahe Zibaee,Mohammad Afshar,Mahmoud Hashemzaei,Gholamreza Karimi
- مشاهده
- جایگاه : پژوهشی
- مجله: Journal of Nanostructures
- نوع مقاله: Journal Article
- کلمات کلیدی: Rhamnolipid,Addition time,Bacteria growth,Biosurfactant production,Nanoparticles concentration
- چکیده:
- چکیده انگلیسی: The application of iron-silica (Fe-Si) nanoparticles for the enhancement of the Pseudomonas aeruginosa growth and rhamnolipid production in molasses medium was studied. The experiments were designed based on the response surface method (RSM) to optimize growth and rhamnolipid production. The concentration of nanoparticles and the time required to add nanoparticles to culture medium were considered as independent variables. The dry weight of cell, the dry weight of rhamnolipid and the surface tension were measured as response variables. In addition, to determine a basic and low-cost medium, the concentrations of molasses and NaCl as components of medium were optimized by RSM. The optimum medium was estimated to include 15% of molasses without NaCl. The results showed that the highest increase in the growth of P. aeruginosa is 25% which occurred at 600 mg/L of nanoparticles and 18 h of addition time compared to the free-nanoparticles experiment. In the same way, the highest increase in rhamnolipid production was 57% at 1 mg/L of nanoparticles and 6 h of addition time compared to blank experiment. TEM images of the morphology changes of bacteria demonstrated the permeation of nanoparticles into the inbound cells. Results of this study reveal the great potential of Fe-Si nanoparticles to overcome the difficulties of the rhamnolipid production in industrial scale.
- انتشار مقاله: 22-07-1396
- نویسندگان: Zahra Sahebnazar,Dariush Mowla,Gholamreza Karimi
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Pharmaceutical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: Aflatoxin,Contamination,Pasteurized milk
- چکیده:
- چکیده انگلیسی: The aim of this study was to evaluate aflatoxin M1 (AFM1) contamination in pasteurized milk samples in Mashhad, Iran. One hundred and ten milk samples from different supermarkets were collected during three months in spring and investigated by Enzyme Linked Immuno Sorbent Assay (ELISA). AFM1 was found in 100% of the milk samples. About 5.4% of the samples contained AFM1 greater than the maximum tolerance limit (0.05 μg/l) accepted by European Union. There was not a significant difference among the mean value of AFM1 in three months.
- انتشار مقاله: 20-12-1385
- نویسندگان: Gholamreza Karimi,Mohammad Hassanzadeh,Marziye Teimuri,Firuzeh Nazari,Amir Nili
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Pharmaceutical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: hemolysis,Rosmarinus officinalis,AAPH
- چکیده:
- چکیده انگلیسی: The oxidative hemolysis of rat erythrocytes induced by 2,2’-azobis–(2-amidinopropane) (AAPH) and its inhibition by rosemary essential oil was studied. Different concentrations (0.178, 0.357, 0.534 and 0.712 ml/ml) of the essential oil showed no significant hemolysis compared to phosphate buffer solution. AAPH (25 mM and 50 mM) induced hemolysis in a time-dependent manner. Diff e r e n t concentrations of the essential oil inhibited hemolysis induced by 25 mM AAPH. However, in the presence of 50 mM of AAPH, only the two higher concentrations (0.534 and 0.712 ml/ml) of the essential oil inhibited hemolysis. Addition of essential oil 2 or 3 h after incubation with AAPH had no significant effects on the time course of cell lysis. It is concluded that, in addition to its well-established antioxidant effects, rosemary essential oil displays cytoprotective properties.
- انتشار مقاله: 26-04-1384
- نویسندگان: Gholamreza Karimi,Mohammad Hassanzadeh,Soghra Mehri
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Basic Medical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: Cytokines,Crocus sativus,Anti-inflammatory,Saffron,Immunomodulatory
- چکیده:
- چکیده انگلیسی: The medicinal uses of saffron, the dried stigmas of Crocus sativus L., have very long history in food coloring agent, and flavoring agent as well as traditional medicine for the treatment of several diseases. Crocus sativus is rich in carotenoids that affect immunity. This review summarizes the putative immunoregulatory effects of saffron and its active its derivatives including crocin, crocetin and safranal. In modern studies, its active constituents including protective effects, anti-inflammatory activities and molecular mechanisms of saffron on the immune system have been demonstrated. Furthermore, the beneficial effects of saffron on inhibition of serum levels nuclear transcription factor κB (NF-κB) p65 unit, tumor necrosis factor alpha (TNF-α), interferon gamma (IFN-γ) and some interleukin (IL) such as IL-1β, IL-6, IL-12, IL-17A were reported. Furthermore, saffron has been known as the antagonist of NF-κB and the agonist of peroxisome proliferator-activated receptor gamma (PPAR-γ). In addition, saffron down-regulates the key pro-inflammatory enzymes such as myeloperoxidase (MPO), cyclooxygenase-2 (COX-2), inducible nitric oxide synthase (iNOS), phospholipase A2, and prostanoids.
This review summarizes the protective roles of C. sativus and its constituents against the pathogenesis of immune diseases and understanding a better management of these problems. Taken together, the main bioactive constituents of saffron may have health-promoting with important benefits in immune-related disorders. Finally, our study indicates that these bioactive constituents can affect both cellular and humoral immunity functions.- انتشار مقاله: 30-05-1397
- نویسندگان: Majid Zeinali,Mohammad Reza Zirak,Seyed Abdolrahim Rezaee,Gholamreza Karimi,Hossein Hosseinzadeh
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Basic Medical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: Cytokines,Crocus sativus,Anti-inflammatory,Saffron,Immunomodulatory
- چکیده:
- چکیده انگلیسی: The medicinal uses of saffron, the dried stigmas of Crocus sativus L., have very long history in food coloring agent, and flavoring agent as well as traditional medicine for the treatment of several diseases. Crocus sativus is rich in carotenoids that affect immunity. This review summarizes the putative immunoregulatory effects of saffron and its active its derivatives including crocin, crocetin and safranal. In modern studies, its active constituents including protective effects, anti-inflammatory activities and molecular mechanisms of saffron on the immune system have been demonstrated. Furthermore, the beneficial effects of saffron on inhibition of serum levels nuclear transcription factor κB (NF-κB) p65 unit, tumor necrosis factor alpha (TNF-α), interferon gamma (IFN-γ) and some interleukin (IL) such as IL-1β, IL-6, IL-12, IL-17A were reported. Furthermore, saffron has been known as the antagonist of NF-κB and the agonist of peroxisome proliferator-activated receptor gamma (PPAR-γ). In addition, saffron down-regulates the key pro-inflammatory enzymes such as myeloperoxidase (MPO), cyclooxygenase-2 (COX-2), inducible nitric oxide synthase (iNOS), phospholipase A2, and prostanoids.
This review summarizes the protective roles of C. sativus and its constituents against the pathogenesis of immune diseases and understanding a better management of these problems. Taken together, the main bioactive constituents of saffron may have health-promoting with important benefits in immune-related disorders. Finally, our study indicates that these bioactive constituents can affect both cellular and humoral immunity functions.- انتشار مقاله: 30-05-1397
- نویسندگان: Majid Zeinali,Mohammad Reza Zirak,Seyed Abdolrahim Rezaee,Gholamreza Karimi,Hossein Hosseinzadeh
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Basic Medical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: oxidative stress,Cardiotoxicity,Silybum marianum,Silymarin,Metals
- چکیده:
- چکیده انگلیسی: Cardiac disorders remain one of the most important causes of death in the world. Oxidative stress has been suggested as one of the molecular mechanisms involved in drug-induced cardiac toxicity. Recently, several natural products have been utilized in different studies with the aim to protect the progression of oxidative stress-induced cardiac disorders. There is a large body of evidence that administration of antioxidants may be useful in ameliorating cardiac toxicity. Silymarin, a polyphenolic flavonoid has been shown to have utility in several cardiovascular disorders. In this review, various studies in scientific databases regarding the preventive effects of silymarin against cardiotoxicity induced by chemicals were introduced. Although there are many studies representing the valuable effects of silymarin in different diseases, the number of researches relating to the possible cardiac protective effects of silymarin against drugs induced toxicity is rather limited. Results of these studies show that silymarin has a broad spectrum of cardiac protective activity against toxicity induced by some chemicals including metals, environmental pollutants, oxidative agents and anticancer drugs. Further studies are needed to establish the utility of silymarin in protection against cardiac toxicity.
- انتشار مقاله: 04-07-1395
- نویسندگان: Bibi Marjan Razavi,Gholamreza Karimi
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Basic Medical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: Silymarin,Formalin test,Inflammatory pain,Sciatic nerve ligation
- چکیده:
- چکیده انگلیسی: Objective(s):Based on the previous reports, silymarin can suppress nitric oxide, prostaglandin E2 (PGE2), leukotrienes, cytokines production, and neutrophils infiltration. Regarding the fact that inflammation plays an important role in neuropathic and formalin-induced pain, it was assumed that silymarin could reduce pain. The present study investigates the analgesic effects of silymarin in chemical nociception and a model of neuropathic pain. Materials and Methods: Chemical nociception was produced by injection of 20 µl of formalin (0.5% formaldehyde in saline) into the plantar region of the right hind paw. A sciatic-nerve ligated mouse was applied as the model of neuropathic pain and the antinociceptive response of silymarin was examined 14 days after unilateral nerve-ligation using the hot plate test. Results:The intraperitoneal administration of silymarin (25, 50, and, 100 mg/kg) 2 hr prior to the intraplantar formalin injection suppressed the nociceptive response during the late phase of the formalin test significantly, but it was not in a dose-dependent manner. Different doses of silymarin 14 days after unilateral sciatic nerve ligation in hot plate test did not induce obvious antinociception. Conclusion:Results of the present study indicated that repeated administration of silymarin prevents the formalin-induced nociceptive behavior. However, it is not effective in the treatment of sciatic neuropathic pain.
- انتشار مقاله: 08-05-1394
- نویسندگان: Faezeh Vahdati Hassani,Ramin Rezaee,Hasan Sazegara,Mahmoud Hashemzaei,Kobra Shirani,Gholamreza Karimi
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Basic Medical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: Medicinal plant,cancer,Liver,Antioxidant,Silymarin
- چکیده:
- چکیده انگلیسی: Widespread use of herbal drugs because of their protective effects on different organs toxicity has been shown in many studies. These protective effects have been illustrated in the fields of nephrotoxicity, hepatotoxicity, viral hepatitis, cancer, in vitro fertilization, neurotoxicity, depression, lung diseases, prostate diseases etc. Silymarin has cytoprotection activities due to its antioxidant activity and radical scavenging. The possible known mechanisms of action of silymarin protection are blockade and adjustment of cell transporters, p-glycoprotein, estrogenic and nuclear receptors. Moreover, silymarin anti-inflammatory effects through reduction of TNF-a, protective effects on erythrocyte lysis and cisplatin-induced acute nephrotoxicity have been indicated in some studies. Silymarin has also inhibited apoptosis and follicular development in patients undergoing IVF. Basis on such data, silymarin can be served as a novel medication in complementary medicine.
- انتشار مقاله: 01-07-1394
- نویسندگان: Gholamreza Karimi,Maryam Vahabzadeh,Parisa Lari,Marziyeh Rashedinia,Mohammad Moshiri
- مشاهده