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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: VETERINARY RESEARCH FORUM
- نوع مقاله: Journal Article
- کلمات کلیدی: Avian Influenza,Adjuvant,Olive,Fig,Nano-selenium
- چکیده:
- چکیده انگلیسی: Influenza is a contagious viral disease that is seen in avian, human and other mammals, so its control is important. Vaccination against influenza virus subtype H9N2 is one of the ways in controlling program, for this reason several vaccines has been produced. Recently, application of inactivated oil-emulsion vaccines in poultry for controlling low pathogenic avian influenza is increasing. At present, oils that are used as adjuvant in commercial vaccines are mineral oils, which not only lack immunizing effect, but also produce some detriments. The aim of this study is the evaluation the immunogenicity of vegetable oils, which are more metabolizable and safer than mineral oils. In this study the efficacy of hexanic extracts of fig (Ficus carica) and olive (Olea europaea) fruit and also nano-selenium on the immunogenicity of the inactivated avian influenza virus subtype H9N2 was evaluated in broiler chickens. The results indicated that the prepared emulsions could elicit a little degree of immunity, but they could not inhibit the anamnestic response and infection. With regard to the results, it seems that the intact mixture of fig and olive fruit hexanic extracts could not be administered as an immunoadjuvant in the vaccine, and about nano-selenium. In spite of positive effect on the immunogenicity of avian influenza virus subtype H9N2, it still needs more work.
- انتشار مقاله: 22-06-1394
- نویسندگان: Amir Hossein Asl Najjari,Zolfaghar Rajabi,Mehdi Vasfi Marandi,Gholamreza Dehghan
- مشاهده
- جایگاه : پژوهشی
- مجله: Nanomedicine Journal
- نوع مقاله: Journal Article
- کلمات کلیدی: Spectroscopy,Dopamine,Iron Oxide Nanoparticles,DNA interaction
- چکیده:
- چکیده انگلیسی: Objective(s): The interaction of DNA with iron oxide nanoparticles (SPIONs) was studied to find out the
interaction mechanism and design new drug delivery systems.
Materials and Methods: The interaction of calf thymus DNA (ctDNA) with SPIONs doped with 2H-chromene
via dopamine as cross linker (SPIONs@DA-Chr) was studied using the UV absorption spectroscopy, viscosity
measurement, circular dichroism, fluorescence and FT-IR spectroscopic techniques.
Results: UV absorption study showed hyperchromic effect in the spectra of DNA. Few changes were
observed in the viscosity of ctDNA in the presence of different concentration of SPIONs@DA-Chr. The result
of circular dichroism (CD) suggested that SPIONs@DA-Chr can change the secondary structure of DNA.
Further, fluorescence quenching reaction of ctDNA with SPIONs@DA-Chr and competitive fluorescence
spectroscopy studied by using methylene blue, have shown that the SPIONs@DA-Chr can bind to ctDNA
through non-intercalative mode. FT-IR spectroscopy confirmed the binding of SPIONs@DA-Chr and
ctDNA.
Conclusion: These results suggested that SPIONs@DA-Chr binds to DNA via groove binding mode.- انتشار مقاله: 04-06-1396
- نویسندگان: Gholamreza Dehghan,Maryam Mehdipour,Mohammad Ali Hosseinpour Feizi,Roghayeh Tarasi,Mehdi Khoobi,Siavoush Dastmalchi
- مشاهده
- جایگاه : پژوهشی
- مجله: Nanomedicine Journal
- نوع مقاله: Journal Article
- کلمات کلیدی: Spectroscopy,Dopamine,Iron Oxide Nanoparticles,DNA interaction
- چکیده:
- چکیده انگلیسی: Objective(s): The interaction of DNA with iron oxide nanoparticles (SPIONs) was studied to find out the
interaction mechanism and design new drug delivery systems.
Materials and Methods: The interaction of calf thymus DNA (ctDNA) with SPIONs doped with 2H-chromene
via dopamine as cross linker (SPIONs@DA-Chr) was studied using the UV absorption spectroscopy, viscosity
measurement, circular dichroism, fluorescence and FT-IR spectroscopic techniques.
Results: UV absorption study showed hyperchromic effect in the spectra of DNA. Few changes were
observed in the viscosity of ctDNA in the presence of different concentration of SPIONs@DA-Chr. The result
of circular dichroism (CD) suggested that SPIONs@DA-Chr can change the secondary structure of DNA.
Further, fluorescence quenching reaction of ctDNA with SPIONs@DA-Chr and competitive fluorescence
spectroscopy studied by using methylene blue, have shown that the SPIONs@DA-Chr can bind to ctDNA
through non-intercalative mode. FT-IR spectroscopy confirmed the binding of SPIONs@DA-Chr and
ctDNA.
Conclusion: These results suggested that SPIONs@DA-Chr binds to DNA via groove binding mode.- انتشار مقاله: 04-06-1396
- نویسندگان: Gholamreza Dehghan,Maryam Mehdipour,Mohammad Ali Hosseinpour Feizi,Roghayeh Tarasi,Mehdi Khoobi,Siavoush Dastmalchi
- مشاهده
- جایگاه : پژوهشی
- مجله: Progress in Biological Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: oxidative stress,Antioxidant enzymes,Spatial Memory,Neurotoxicity
- چکیده:
- چکیده انگلیسی: Paraquat (PQ), is one of the most widely used herbicides all over the world. PQ could induce dopaminergic cell death. Since dopamine involves in memory processing, we investigated the recovery effect of vitamin C on spatial memory along with oxidative stress parameters during PQ induced neurotoxicity in male rats. Rats were divided into five groups (n= 7): control (saline 0.9%), PQ (2.67 and 5 mg/kg), vitamin C (80 mg/kg) plus PQ (2.67), and vitamin C plus PQ (5 mg/kg). The period of intraperitoneal injection (i.p.) was once a day and for 5 consecutive days. The Morris water maze test used for studying the spatial memory. The level of lipid peroxidation (MDA), and activity of antioxidant enzymes; superoxide dismutase (SOD) and catalase (CAT), were determined in the left hemisphere of rats. Results showed that i.p. injection of PQ in both doses, 2.67 mg/kg (P<0.05) and 5mg/kg (P<0.01) significantly decreased the spatial memory. The total SOD activity in PQ-treated groups (2.67 and 5mg/kg) was significantly lower than that of control group (p<0.01). The level of CAT increased, in Vitamin C plus PQ groups in a dose-dependently manner (p<0.05). MDA was significantly increased in PQ-treated group (p<0.01). In PQ-treated groups that were supplemented with vitamin C, SOD activity and lipid peroxidation level were restored to normalcy. Our data revealed that PQ could impair the spatial memory via induction of oxidative stress in the brain tissue. Vitamin C can prevent or diminish the oxidative stress markers in the PQ-treated rats.
- انتشار مقاله: 27-02-1395
- نویسندگان: Hassan Saei,Homeira Hatami,Omid Purbagheriyan,Seyed Mohammad Hosseini,Gholamreza Dehghan
- مشاهده
- جایگاه : پژوهشی
- مجله: Avicenna Journal of Phytomedicine
- نوع مقاله: Journal Article
- کلمات کلیدی: Genotype,Radical Scavenging,storage,Almond,Hull,Shell
- چکیده:
- چکیده انگلیسی: Objectives: Almond (Prunus amygdalus) hull and shell are agricultural by-products that are a source of phenolic compounds.The processing of almond produce shell and hull, accounts for more than 50% by dry weight of the almond fruits. Recently, more studies have focused on the influence of storage conditions and postharvest handling on the nutritional quality of fruits, especially the antioxidant phenolics. In this study, influence of long-term storage (five years) on the total phenolic and antioxidant capacity of almond hull and shell from different genotypes was evaluated.
Materials and Methods: The fruits of subjected genotypes were collected and their hull and shell were separated. They were dried and reduced to fine powder. This powder stored at room temperature for five years. The total phenolic content (TPC) and bioactivities (antioxidant potential: DPPH and ABTS radical scavenging and reducing power) of extracts were evaluated using spectrophotometric methods.
Results: It was found that TPC content and bioactivity levels in the stored almond hull and shell were different, compared to the hulls and shells which were evaluated in 2007. S1-4 genotype had the highest TPC and reducing power in its hull and shell.Low correlation coefficient was observed between phenolic content and the DPPH radical scavenging percentage in hull and shell extract.
Conclusions: For the first time, results of this investigation showed that storage can influence the antioxidant and antiradical potential of almond hull and shell.- انتشار مقاله: 19-10-1392
- نویسندگان: Khadijeh Sadat Moosavi Dolatabadi,Gholamreza Dehghan,Siavash Hosseini,Ali Jahanban Esfahlan
- مشاهده
- جایگاه : پژوهشی
- مجله: Advanced Herbal Medicine
- نوع مقاله: Journal Article
- کلمات کلیدی: GC/MS,Essential oils,Elaeagnus angustifolia,Esters,Ketones
- چکیده:
- چکیده انگلیسی: Background and aims: Elaeagnus angustifolia L. is a member of the Elaeagaceae family; different parts of it, especially fruits have been used for the treatment of several diseases in traditional medicine. The aim of this study was to isolate and determine essential oil composition of flowers of E. angustifolia collected from different ecological areas of East-Azarnayjan in Iran.
Methods: In this experimental study, the essential oils of the flower were isolated by hydrodistillation method and analyzed by GC and GC/MS.
Results: The number of compounds in the essential oil isolated from the population of Ahar, Marand and Hashtroud were 22, 17 and 14, respectively. The major component of all of the populations was ethylcinnamate; Ahar (47.59%), Marand (69.99%) and Hashtroud (85.49%). It was observed that the oil number of E. angustifolia decreases from 22 to 14 when the altitude increases from 1344-1750 m.
Conclusion:Chemical composition of the essential oils of E. angustifolia L. such as esters and aromatic acids contents were increased while the ketone content was decreased with increasing altitude.- انتشار مقاله: 06-12-1394
- نویسندگان: Faranak Elmi,Gholamreza Dehghan,Behnam Beigzadeh
- مشاهده
- جایگاه : پژوهشی
- مجله: Advanced Herbal Medicine
- نوع مقاله: Journal Article
- کلمات کلیدی: Achillea,1, 8-Cineole,essential oil,GC/MS,α-Terpinen
- چکیده:
- چکیده انگلیسی: Background and aims: The Achillea genus has a wide distributional range, and the differences in oil composition may be affected by different environmental factors such as plant genetic type, seasonality, and developmental stage. The aim of this research was to determine the chemical combination of the essential oils derived from flowering aerial parts of Achillea millefolium, A. biebersteinii and A. wilhelmsii (family Astreacea) collected from different locations of East-Azarbayjan in Iran. Methods: In this experimental study, Wild Achillea plants (A. millefolium, A. biebersteinii and A. wilhelmsii) were collected from three localities of East-Azarbayjan of Iran during the flowering period. The chemical combination of the isolated oils was examined by gas chromatography-mass spectrometry. Results: In A. millefolium, the major compounds were 1,8-cineole (28.0%), camphor (19.2%), borneol (98.8%) and β-pinene (6.3%). In A. biebersteinii the major compounds were α-terpinen (41.42%), 2-carene (13.96%), m-cymene (13.41%) and 1,8-cineole (8.91%). In A. wilhelmsii the major compounds were carvacrol (29.2%), linalool (10.3%), 1,8-cineole (11.0%), (E)-nerolidol (8.4%) and borneol (5.04%). Conclusion: Chemical compounds of essential oils of Achillea species were highly variable, which may be due to the differences in their chemical polymorphic structure and environmental conditions.
- انتشار مقاله: 13-06-1393
- نویسندگان: Gholamreza Dehghan,Faranak Elmi
- مشاهده
- جایگاه : پژوهشی
- مجله: Advanced Herbal Medicine
- نوع مقاله: Journal Article
- کلمات کلیدی: Achillea,1, 8-Cineole,essential oil,GC/MS,α-Terpinen
- چکیده:
- چکیده انگلیسی: Background and aims: The Achillea genus has a wide distributional range, and the differences in oil composition may be affected by different environmental factors such as plant genetic type, seasonality, and developmental stage. The aim of this research was to determine the chemical combination of the essential oils derived from flowering aerial parts of Achillea millefolium, A. biebersteinii and A. wilhelmsii (family Astreacea) collected from different locations of East-Azarbayjan in Iran. Methods: In this experimental study, Wild Achillea plants (A. millefolium, A. biebersteinii and A. wilhelmsii) were collected from three localities of East-Azarbayjan of Iran during the flowering period. The chemical combination of the isolated oils was examined by gas chromatography-mass spectrometry. Results: In A. millefolium, the major compounds were 1,8-cineole (28.0%), camphor (19.2%), borneol (98.8%) and β-pinene (6.3%). In A. biebersteinii the major compounds were α-terpinen (41.42%), 2-carene (13.96%), m-cymene (13.41%) and 1,8-cineole (8.91%). In A. wilhelmsii the major compounds were carvacrol (29.2%), linalool (10.3%), 1,8-cineole (11.0%), (E)-nerolidol (8.4%) and borneol (5.04%). Conclusion: Chemical compounds of essential oils of Achillea species were highly variable, which may be due to the differences in their chemical polymorphic structure and environmental conditions.
- انتشار مقاله: 13-06-1393
- نویسندگان: Gholamreza Dehghan,Faranak Elmi
- مشاهده