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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: Iranian Journal of Biotechnology
- نوع مقاله: Journal Article
- کلمات کلیدی: siRNA,Multidrug Resistance,protein 1,HL60
- چکیده:
- چکیده انگلیسی: Multidrug resistance (MDR) is a complex phenomenon in which many different genes regulating drug transport, cellular repair, detoxification and drug metabolism are involved. Nevertheless, in most drug resistant cell lines and cancer patients up-regulation of ABC-transporter genes such as MDR associated Protein (MRP1) gene could be at the basis of the drug resistance phenotype. We aimed to decrease MRP1 expression at the mRNA level to modulate drug resistance phenotype in the methotrexate-resistant HL60 cell line. We designed a small interfering RNA (siRNA) molecule against MRP1 and applied it to HL60 cell line in a 0 to 72 hours time range. siRNA could specifically inhibit gene expression by 80% of the initial mRNA level with in 36 to 48 hours. The siRNA-treated cells demonstrated 100-fold reduction in methotrexate (MTX) resistance compared to untreated cells. The data indicate that this approach may be applicable to the study of MRP1 expression and development of future strategies to reverse the MRP1 dependent drug-resistance phenotype in tumors back to a drug-sensitive one.
- انتشار مقاله: 10-06-1394
- نویسندگان: Masoud Golalipour,Frouzandeh Mahjoubi,Mohammad Hossein Sanati
- مشاهده
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: Breast cancer,Multidrug Resistance,LRP,MVP,MRP1
- چکیده:
- چکیده انگلیسی: Background: Breast cancer is the most common malignancy in women. Multidrug resistance (MDR) is still a great
obstacle of breast cancer chemotherapy. We have previously shown that multidrug resistance-associated protein 1 (MRP1)
is associated with response to neoadjuvant chemotherapy. The lung resistance-related protein (LRP) is identified as
a prognostic marker and response to treatment factor which has been studied mainly in hematological malignancy and
leukemia. In this study, we aimed to analyze LRP expression and possible correlation between the expression level of
this gene with MRP1 as a candidate marker for chemotherapy resistance. Materials and Methods: We collected 54
breast tumors and adjacent normal tissues from Iranian breast cancer patients and Real time RT-PCR was employed to
measure the gene expression level in our samples. Results: MRP1 and LRP expression level were significantly lower
in tumor tissues of the patients responding to chemotherapy compared to non-responding patients. No relation between
the expression level of either of these genes and clinicopathology markers was found. Conclusion: Our results suggest
that LRP gene expression is correlated to MRP1 in human breast cancer cells and may affect the clinical response to
treatment.- انتشار مقاله: 13-10-1396
- نویسندگان: Mohsen Taheri,Jamshid Motalebzadeh,Frouzandeh Mahjoubi
- مشاهده
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: Colorectal cancer,Biomarker,Gene expression,Prognostic marker,Lymph node metastasis
- چکیده:
- چکیده انگلیسی: Background: Colorectal cancer (CRC) is one of the most prevalent cancers worldwide. Despite recent progress in
diagnosis and treatment, it remains a major health problem and further studies are needed. We here investigated expression
profiles of the FBXO39, ETS-1 and BMI-1 genes in CRCs to validate any possible diagnostic/prognostic significance.
Material and Methods: Thirty six patients with locally advanced CRC admitted to Hazrate-Rasoul Hospital-Tehran
were enrolled. Initially the expression pattern of FBXO39, ETS-1 and BMI-1 genes were determined using RT-PCR
in CRC tumor and adjacent normal tissues then real-time RT-PCR was employed to quantify BMI-1 gene expression.
Results: FBXO39 expression was restricted to tumor tissues. Interestingly, expression of this gene was detected in all
stage-0 tumor samples. There was a significant relation between FBXO39 gene expression and lymph node involvement.
The ETS-1 gene was expressed in 66% of all tumor tissues with p-value=0.03 for increase as compared to the adjacent
normal samples. In addition, there was a significant relation between ETS-1 gene expression and tumor size and lymph
node involvement. RT-PCR demonstrated BMI-1 gene expression in both tumor and normal tissues and quantification
by real-time RT-PCR showed no association between BMI-1 levels and CRC clinicopathological features. Conclusion:
Expression of FBXO39 and ETS-1 with lymph node involvement may be considered as an alarm for the occurrence
of CRC metastasis, and therfore have prognostic value while BMI-1 appears without importance.- انتشار مقاله: 13-10-1396
- نویسندگان: Jamshid Motalebzadeh,Samira Shabani,Saeedeh Rezayati,Narges Shakournia,Rezvan Mirzaei,Bahar Mahjoubi,Kamal Hoseini,Frouzandeh Mahjoubi
- مشاهده