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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: Colon cancer,Zebularine,trichostatin,CIP/KIP family
- چکیده:
- چکیده انگلیسی: Background: A pattern of epigenetic modifications and changes, DNA methylation and histone modification, is central to many human cancers. A variety of tumor suppressor genes (TSGs) have been demonstrated to be silenced because of histone deacetylation and DNA hypermethylation in several cancers. Recent in vitro studies have shown that two known mechanisms of epigenetic alteration consisting of methylation and histone deacetylation seem to be the best candidate mechanisms for inactivation of CIP/KIP family (p21Cip1/Waf1/Sdi1, and p27Kip1) in numerous cancers. Numerous investigations have indicated that DNA demethylating and histone deacetylase inhibitors (HDACIs) can restore the CIP/KIP family gene expression. Previously, we evaluated the effect of trichostatin A (TSA) and 5-aza-2′-deoxycytidine (5-AZA-CdR) on hepatocellular carcinoma (HCC). The present study was designed to investigate the effect of zebularine in comparison to and in combination with trichostatin A on p21Cip1/Waf1/Sdi1, p27Kip1, p57Kip2, DNMT1, DNMT3a and DNMT3b, Class I HDACs (HDACs 1, 2, 3) and Class II HDACs (HDACs 4, 5, 6) gene expression, cell growth inhibition and apoptosis induction in colon cancer LS 174T cell line. Materials and Methods: The colon cancer LS 174T cell line was cultured and treated with zebularine and TSA. To determine cell viability, apoptosis, and the relative expression level of the genes, MTT assay, cell apoptosis assay, and qRT-PCR were done respectively. Results: Both compounds significantly inhibited cell growth, and induced apoptosis. Furthermore, both compounds increased p21Cip1/Waf1/Sdi1, p27Kip1, and p57Kip2 significantly. Additionally, zebularine and TSA decreased DNMTs and HDACs gene expression respectively. Conclusion: The zebularine and trichostatin A can reactivate the CIP/KIP family through inhibition of DNMTs and HDACs genes activity.
- انتشار مقاله: 20-01-1399
- نویسندگان: Masumeh Sanaei,Fraidoon Kavoosi
- مشاهده
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: Apoptosis,Proliferation,Valproic acid,Hepatocellular carcinoma,Genistein
- چکیده:
- چکیده انگلیسی: Background: DNA demethylating agents and histone deacetylase inhibitors can affect reactivation of gene
expression and apoptosis induction by DNA acetylation and demethylation. The aim of the present study was to analyze
the effects of DNA demethylating agent genistein (GE) and histone deacetylase inhibitor valproic acid VPA), alone
and combined, on hepatocellular carcinoma Hep G2 cell line. Methods: The cells were treated with various doses of
genistein and valproic acid (alone and combined) and the MTT assay and flow cytometry were used to determine cell
viability and apoptosis. Results: Genistein and valproic acid inhibited the growth of HepG 2 cells significantly. Result
of flow cytometry demonstrated that genistein and valproic acid (alone and combined) induce apoptosis significantly in
a time‑dependent manner. Conclusions: Genistein and valproic acid can significantly inhibit proliferation and induce
apoptosis in HepG2 cell line. The apoptotic effects of GE in combination with VPA were more significant that of each
compound alone.- انتشار مقاله: 12-06-1397
- نویسندگان: Masumeh Sanaei,Fraidoon Kavoosi
- مشاهده
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: Viability,Apoptosis,Estradiol,Hepatocellular carcinoma
- چکیده:
- چکیده انگلیسی: Background: Phytoestrogens are a group of natural compounds with estrogen-like activity and similar structure
to estradiol that structurally mimic the mammalian estrogen 17-β estradiol (E2). They have a biphasic effect and exert
pleiotropic effects which induce or inhibit estrogen action by activation/inhibition of the estrogen receptors (ERs).
These compounds can induce apoptosis at high concentrations. The previous finding indicated that E2 inhibited cell
growth and induced apoptosis in hepatocellular carcinoma (HCC) PLC/PRF/5 cell line. The aim of the present study
was to investigate the apoptotic and proliferative effects of E2 on hepatocellular carcinoma HepG 2 and LCL-PI 11
cells. Methods: The Hep G2 and LCL-PI 11 cells were cultured and treated with E2 for different time periods and then
MTT [3-(4, 5-dimethyl-2-thiazolyl) -2, 5-diphenyl -2H- tetrazolium bromide] assay and flow cytometry assay were
done to determine cell viability and cell apoptosis respectively. Results: E2 had inhibitory and apoptotic effects on Hep
G2 cell line, whereas it indicated a biphasic effect on LCL-PI 11 cell line. The half-maximum inhibitory concentration
(IC50) value was 3 μM. The inhibitory effect of E2 on Hep G2 cells was observed with all concentrations of E2 (P
<0.087), whereas E2 showed a biphasic effect on LCL-PI 11. This compound induced significant apoptosis in Hep G2
cell line at the all treatment times versus control groups, whereas, in the LCL-PI 11 cell, significant apoptotic cells were
observed after 72 and 96h (P <0.001). Conclusion: E2 can inhibit cell growth and induce apoptosis in hepatocellular
carcinoma HepG 2 and LCL-PI 11 cell lines.- انتشار مقاله: 25-02-1397
- نویسندگان: Masumeh Sanaei,Fraidoon Kavoosi,Faeze Dehghani
- مشاهده
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: Apoptosis,Proliferation,Valproic acid,Hepatocellular carcinoma
- چکیده:
- چکیده انگلیسی: The nucleosome is the fundamental building block of eukaryotic chromatin formed by DNA and histone proteins.
Chromatin modifications such as acetylation, methylation, and phosphorylation are necessary for protection,
replication, and gene transcription. Histone deacetylases (HDACs) are a group of enzymes that remove acetyl groups
to re-establish positive charges on histones and aberrant deacetylation may lead to tumorigenesis in different tissues.
Histone deacetylase inhibitors (HDACIs) are a class of chemotherapeutic agent that can reactivate gene expression
and inhibit the growth of tumor cells by histone deacetylase inhibition. HDACI valproic acid (VPA) has shown potent
anticancer effects in vitro and in vivo. Previously, we reported that VAP can inhibit the growth and induce apoptosis
of human colon carcinoma HT 29 and hepatocellular carcinoma HepG 2 cells. The aim of the present study was to
access the effect of VPA on proliferation and apoptosis of the human hepatocellular carcinoma (HCC) PLC/PRF5 cell
line. Materials and Methods: PLC/PRF5 cells were treated with VPA and then MTT and flow cytometry assays were
used to determine the effects on viability and apoptosis, respectively. Results: VPA inhibited cell growth and induced
apoptosis in PLC/PRF5 cells significantly. Discussion: Our results clearly demonstrated that VPA has inhibitory and
apoptotic effects. Conclusion: VPA can significantly inhibit the growth of HCC cells and play a significant role in
apoptosis induction.- انتشار مقاله: 03-09-1396
- نویسندگان: Masumeh Sanaei,Fraidoon Kavoosi,Abazar Roustazadeh,Hossein Shahsavani
- مشاهده
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: Apoptosis,Proliferation,Hepatocellular carcinoma,Genistein,Tamoxifen
- چکیده:
- چکیده انگلیسی:
Introduction: The flavonoids comprise a diverse group of polyphenolic compounds with antioxidant activity that is present in edible plants like soybeans and soy products. In vivo studies have concentrated on the effects of flavonoids on cancer and genistein (GE), a soy-derived isoflavone, has been reported to reduce prostate, colon, hepatic and breast adenocarcinoma risk. Tamoxifen (TAM) is an important drug for cancer treatment worldwide, which can induce apoptosis in various cancers, including examples in the liver, breast and ovaries. The aim of the present study was to evaluate the effects of GE and TAM, alone and in combination, on proliferation and apoptosis in the human hepatocellular carcinoma (HCC) HepG2 cell line. Materials and Methods: HepG 2 cells were treated with GE, TAM and GE/TAM and then MTT and flow cytometry assays were conducted to determine effects on viability and apoptosis, respectively. Results: GE and TAM inhibited cell proliferation and induced apoptosis in the HepG 2 cell lines. Discussion: Our findings clearly indicated that GE and TAM may exert inhibitory and apoptotic effects in liver cancer cells. Conclusion: GE and TAM can significantly inhibit growth of HCC cells and play a significant role in apoptosis.- انتشار مقاله: 26-12-1395
- نویسندگان: Masumeh Sanaei,Fraidoon Kavoosi,Shekoufeh Atashpour,Soheila Haghighat
- مشاهده
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: Apoptosis,Proliferation,Hepatocellular carcinoma,Genistein,Tamoxifen
- چکیده:
- چکیده انگلیسی:
Introduction: The flavonoids comprise a diverse group of polyphenolic compounds with antioxidant activity that is present in edible plants like soybeans and soy products. In vivo studies have concentrated on the effects of flavonoids on cancer and genistein (GE), a soy-derived isoflavone, has been reported to reduce prostate, colon, hepatic and breast adenocarcinoma risk. Tamoxifen (TAM) is an important drug for cancer treatment worldwide, which can induce apoptosis in various cancers, including examples in the liver, breast and ovaries. The aim of the present study was to evaluate the effects of GE and TAM, alone and in combination, on proliferation and apoptosis in the human hepatocellular carcinoma (HCC) HepG2 cell line. Materials and Methods: HepG 2 cells were treated with GE, TAM and GE/TAM and then MTT and flow cytometry assays were conducted to determine effects on viability and apoptosis, respectively. Results: GE and TAM inhibited cell proliferation and induced apoptosis in the HepG 2 cell lines. Discussion: Our findings clearly indicated that GE and TAM may exert inhibitory and apoptotic effects in liver cancer cells. Conclusion: GE and TAM can significantly inhibit growth of HCC cells and play a significant role in apoptosis.- انتشار مقاله: 26-12-1395
- نویسندگان: Masumeh Sanaei,Fraidoon Kavoosi,Shekoufeh Atashpour,Soheila Haghighat
- مشاهده
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: Apoptosis,Proliferation,Hepatocellular carcinoma,Genistein,Tamoxifen
- چکیده:
- چکیده انگلیسی:
Introduction: The flavonoids comprise a diverse group of polyphenolic compounds with antioxidant activity that is present in edible plants like soybeans and soy products. In vivo studies have concentrated on the effects of flavonoids on cancer and genistein (GE), a soy-derived isoflavone, has been reported to reduce prostate, colon, hepatic and breast adenocarcinoma risk. Tamoxifen (TAM) is an important drug for cancer treatment worldwide, which can induce apoptosis in various cancers, including examples in the liver, breast and ovaries. The aim of the present study was to evaluate the effects of GE and TAM, alone and in combination, on proliferation and apoptosis in the human hepatocellular carcinoma (HCC) HepG2 cell line. Materials and Methods: HepG 2 cells were treated with GE, TAM and GE/TAM and then MTT and flow cytometry assays were conducted to determine effects on viability and apoptosis, respectively. Results: GE and TAM inhibited cell proliferation and induced apoptosis in the HepG 2 cell lines. Discussion: Our findings clearly indicated that GE and TAM may exert inhibitory and apoptotic effects in liver cancer cells. Conclusion: GE and TAM can significantly inhibit growth of HCC cells and play a significant role in apoptosis.- انتشار مقاله: 26-12-1395
- نویسندگان: Masumeh Sanaei,Fraidoon Kavoosi,Shekoufeh Atashpour,Soheila Haghighat
- مشاهده