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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: Breast cancer,Colorectal cancer,Autophagy,Camel Milk
- چکیده:
- چکیده انگلیسی: Background/ Objective: Camel milk is traditionally known for its human health benefits and believed to be a remedy
for various human ailments including cancer. The study was aimed to evaluate the inhibitory effects of commercially
available camel milk on cancer cells and its underlying mechanism(s). Materials and Methods: Two cell lines:
colorectal cancer HCT 116 and breast cancer MCF-7 were cultured with different doses of camel milk. The effects of
camel milk on cell death were determined by MTT assay, viability by trypan blue exclusion assay and migration by in
vitro scratch assay. The mechanism was elucidated by western blotting and confocal microscopy was used to confirm
autophagy. Results: Camel milk significantly reduced proliferation, viability as well as migration of both the cells.
The accumulation of LC3-II protein along with reduction in expression of p62 and Atg 5-12, the autophagy proteins
implied induction of autophagy. The (GFP)-LC3 puncta detected by confocal microscopy confirmed the autophagosome
formation in response to camel milk treatment. Conclusion: Camel milk exerted antiproliferative effects on human
colorectal HCT 116 and breast MCF-7 cancer cells by inducing autophagy.- انتشار مقاله: 31-01-1397
- نویسندگان: Roopesh Krishnankutty,Ahmad Iskandarani,Lubna Therachiyil,Shahab Uddin,Fouad Azizi,Michael Kulinski,Ajaz Ahmad Bhat,Ramzi M Mohammad
- مشاهده