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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: Iranian Journal of Immunology
- نوع مقاله: Journal Article
- کلمات کلیدی: Breast cancer,4-1BBL,B Cells,Tumor Draining Lymph Nodes
- چکیده:
- چکیده انگلیسی: Background: B cells can increase the expression of granzyme B in CD8+ T cells through 4-1BBL/4-1BB interaction and promote anti-tumor immunity. Objective: To investigate the expression of 4-1BBL on B cells in the breast tumor draining lymph nodes (TDLNs) and its association with disease parameters. Methods: Using Ficoll-Hypaque gradient centrifugation, mononuclear cells were isolated from axillary lymph nodes of 42 patients. Cells received 4 hours of PMA/Ionomycin stimulation, in vitro. Both unstimulated and stimulated cells were stained with anti‒CD19 and anti‒4-1BBL antibodies and subjected to flow cytometry. Results: 4-1BBL expression was detected on 2.8 ± 1.7% of unstimulated B cells, while 27.4 ± 11.9% of B cells expressed this co-stimulatory molecule following stimulation. In steady state, the percentage of 4-1BBL+ B cells was not associated with cancer characteristics. However, in patients with invasive ductal carcinoma, the percentage of 4-1BBL expressing B cells in stimulated condition had a decreasing trend in grade III, compared to grade II+I. In addition, significantly higher frequency of 4-1BBL+ B cells was seen in the TDLNs of ER+ or PR+ compared with ER‒ or PR‒ patients (p=0.021 and p=0.015, respectively). No significant associations were observed between the frequency of 4-1BBL+ B cells and the number of involved LNs, Her2 expression or disease stage. Conclusions: The frequency of 4-1BBL+ B cells significantly increased following a short time activation, and showed relative and significant associations with tumor grade and estrogen receptor status, respectively. More investigations are required to evaluate the potential of 4-1BBL+ B cells for use in immunotherapy.
- انتشار مقاله: 19-10-1397
- نویسندگان: Mohsen Arabpour,Atri Ghods,Mahmoud Shariat,Abodl-Rasoul Talei,Fereshteh Mehdipour,Abbas Ghaderi
- مشاهده
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: Breast cancer,Cell line establishment,Triple-negative
- چکیده:
- چکیده انگلیسی: Breast cancer is the most common malignancy and the leading cause of cancer-related death among women
worldwide. The underlying mechanisms for breast cancer development, especially in young women, are not completely
understood. Although there are several experimental models to understand the biology of breast cancer such as
immortalized cell lines, many of these cell lines have been in culture for decades and most of them have been derived
from Caucasians or African-Americans. So, it is required to establish a new cell line derived from primary tumors and
Asian women. In this study Pari-Institute for Cancer Research (Pari-ICR) was derived from the primary breast tumor
of a 36-years old patient with invasive ductal carcinoma. We characterized the cell line by examining morphology,
expression of different markers, and functional profile. Immunocytochemistry showed that this cell line does not express
estrogen and progesterone receptors as well as human epidermal growth factor receptor 2 (HER2). Pari-ICR cell line
expresses high levels of Vimentin, Ezrin, and S100 but does not express EpCAM, Cytokeratin19, Pan-cytokeratin,
Nestin, and Desmin. Its doubling time of Pari-ICR was about 22h and was able to grow as colonies in soft agar. It
displayed a higher ability of migration and invasion in comparison with MCF-7 cell line. This breast cancer cell line
can serve as a model for understanding the molecular mechanisms of breast carcinogenesis. Moreover, it can be used
as an appropriate resource to find novel biomarkers or assess new drugs.- انتشار مقاله: 10-05-1397
- نویسندگان: Farzaneh Ghaderi,Fereshteh Mehdipour,Ahmad Hosseini,Abdolrasoul Talei,Abbas Ghaderi
- مشاهده