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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: Iranian Journal of Immunology
- نوع مقاله: Journal Article
- کلمات کلیدی: Immunization,ELISA,Diphtheria-Tetanus-Pertussis Vaccine
- چکیده:
- چکیده انگلیسی: Background: Immunization against diphtheria, tetanus and pertussis has been applied in Iran since 1950. WHO suggests periodical evaluation of effectiveness of the triple diphtheria-tetanus-whole cell pertussis (DTwP) vaccine, worldwide.
Objectives: To determine the immunogenicity of locally manufactured DTwP vaccine administered to preschool children in a number of health centers of Tehran in 2006.
Methods: In this prospective study, 350 children aged 4-6 years were injected with DTwP vaccine manu-factured by Razi Institute of Iran. Blood samples were collected before and 2-4 weeks after the vaccination. The immunogenicity of the vaccine was assayed by measurement of specific antibodies using enzyme-linked immunosorbent assay (ELISA) technique.
Results: Of the 337 children who were vaccinated, 99.4% and 100% had protective anti-diphtheria and anti-tetanus antibody titers, respectively. The vaccine response and seroconversion for pertussis was achieved in 70.3% of the subjects. The geometric mean titers (GMT) of the antibodies produced against diphtheria, tetanus and pertussis by DTwP vaccine were 7.76, 9.37 IU/ml and 30.20 EU/ml after booster vaccine dose, respectively.
Conclusions: Comparison of the results obtained from this study with those of previous studies performed in other countries reveals that immunogenicity of diphtheria and tetanus components is similar to other vaccines, but the immunogenicity of pertussis vaccine was less efficient. The lower immunogenicity of DTwP against pertussis may be related to the bacterial strain used or the formulation protocol adopted for the vaccine preparation.- انتشار مقاله: 17-05-1395
- نویسندگان: Saeed Zarei,Mahmood Jeddi-Tehrani,Mohammad Mehdi Akhondi,Hojjat Zeraati,Tahere Kheirkhah,Morteza Ghazanfari,Fazel Shokri
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Immunology
- نوع مقاله: Journal Article
- کلمات کلیدی: Flow cytometry,ALL,Immunophenotyping,Pro-B,Pre-B
- چکیده:
- چکیده انگلیسی: Background: Immunophenotypic characterization of the leukemic cells has been widely used as a tool for diagnosis, classification, stratification and prognosis of leukaemia.
Objective: To investigate the immunophenotypic subtype profiles of Iranian patients with acute lymphoblastic leukemia (ALL) and its association to disease outcome.
Methods: In this study, a total of 60 Iranian patients with ALL were immunophenotyped by flow cytometry using a panel of monoclonal antibodies specific for CD2, CD3, CD5, CD10, CD13, CD14, CD19, CD20, CD33, CD34, CD45, HLA-DR and TdT molecules.
Results: The samples were initially categorized into T-ALL (n=9), B-ALL (n=50) and mixed lineage (n=1) based on the expression patterns of CD3 and CD19 molecules. B-ALL patients could further be classified into four subtypes, including Pro-B (n=7, 11.7%), Pre-B I (n=28, 46.7%), Pre-B II (n=13, 21.7%) and immature/mature B cells (n=2, 3.3%) on the basis of expression of CD10, CD19, CD20, HLA-DR and TdT. Clinical manifestations and laboratory findings of the patients did not reveal association with immunophenotypic sub-types of ALL, with the exception of mediastinal mass and WBC count at the time of diag-nosis which were found to be significantly higher in patients with T-ALL compared with B-ALL (p=0.001 and 0.014), respectively.
Conclusion: Our results indicate that overall the immunophenotypic profile of Iranian ALL patients is similar to previous reports and it might be used for monitoring of minimal residual disease and prognosis.- انتشار مقاله: 17-05-1395
- نویسندگان: Hossein Asgarian Omran,Mahdi Shabani,Tahereh Shahrestani,Abdolfattah Sarafnejad,Jalal Khoshnoodi,Parvaneh Vossough,Mohammad Faranoush,Ramzan A. Sharifian,Mahmood Jeddi-Tehrani,Hodjatallah Rabbani,Fazel Shokri
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Immunology
- نوع مقاله: Journal Article
- کلمات کلیدی: RT-PCR,Antigen,Wilm’s Tumor Gene 1,Acute Myeloblastic Leukemia,Tumorassociated
- چکیده:
- چکیده انگلیسی: Background: The Wilm’s tumor gene 1 (WT1) encodes a zinc finger transcription factor that is inactivated in a subset of Wilm’s tumors. It plays a crucial role in growth, proliferation and development of some embryonic and adult organs. WT1 is expressed as a tumor associated antigen (TAA) in various types of solid and hematopoietic malignancies and can be employed as a useful marker for targeted immunotherapy and monitoring of minimal residual disease (MRD).
Objective: To investigate the profile of WT1 gene expression in Iranian patients with acute myeloblastic leukemia.
Methods: RT-PCR method was used to determine the WT1 gene expression in bone marrow (BM) and/or peripheral blood (PB) samples from 11 patients with AML and PB samples of 36 normal subjects. Isolated cells from all patients were immunophenotyped by flow cytometry.
Results: The leukemic cells from 10 patients (91%) were found moderately or strongly positive for WT1 expression whereas only 3 out of 36 normal subjects expressed WT1 at very low levels. A highly significant correlation was observed for WT1 expression between paired BM and PB samples of the AML patients.
Conclusion: Our results indicate that WT1 is expressed in the majority of Iranian AML patients and may be employed for screening and monitoring of minimal residual disease in these patients.- انتشار مقاله: 16-05-1395
- نویسندگان: Hossein Asgarian,Mahdi Shabani,Parvaneh Vosoogh,Ramazan Ali Sharifian,Soheila Gharagozlou,Jalal Khoshnoodi,Tahereh Shahrestani,Mahin Kordmahin,Abdolfattah Sarrafnejad,Mahmood Jeddi Tehrani,Hodjatallah Rabbani,Fazel Shokri
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Immunology
- نوع مقاله: Journal Article
- کلمات کلیدی: ELISA,Immunoblotting,Monoclonal antibody,Human IgA2,Conformational Epitope,Linear Epitope
- چکیده:
- چکیده انگلیسی: Background: There are two subclasses of human IgA (IgA1 and IgA2) that differ in antigenic properties and in chemical composition. The constant domains of α1 and α2 heavy chains have >95% sequence homology though major structural differences exist in the hinge region. Quantitation of IgA subclass levels depends on the availability of monoclonal antibodies (MAbs) specific for conserved conformational or linear epitopes restricted to each subclass.
Objective: To produce, select and characterize monoclonal antibodies specific for human IgA2.
Methods: Splenocytes from BALB/C mice immunized with a human IgA2 myeloma protein were fused with SP2/0 myeloma cells. Fused cells were grown in hypoxanthine, aminopterine and thymidine (HAT) selective medium and cloned by limiting dilution assay. Antibody (Ab) secreting cells were screened by enzyme-linked immunosorbent assay (ELISA) and the specificity of secreted MAbs was further analyzed, using a panel of purified myeloma proteins and some animal sera by ELISA and immunoblotting. The affinity constant (Kaff) was also determined by ELISA.
Results: Four murine hybridoma clones designated 2F20G5, 2F20B5, 3F20E3 and 6F20H11 were obtained that secreted MAbs specific for the human IgA2. 2F20G5 and 6F20H11 MAbs react with linear epitope(s) while 2F20B5 and 3F20E3 react with conformational epitope(s) located to human IgA2 subclass. 2F20G5 MAb displays a weak cross-reactivity with monkey and rabbit sera and a strong cross-reactivity with cat and dog sera while the other three MAbs showed no cross-reactivity with the animal sera tested.
Conclusion: These MAbs, especially 6F20H11 with high affinity constant (6.03 ×109 M-1) are useful tools for quantitation of human IgA2 subclass levels in various diseases. Cross-reactivity of 2F20G5 MAb with some animalsera suggests phylogenic conservation ofthe epitope recognized by this MAb.- انتشار مقاله: 16-05-1395
- نویسندگان: Fatemeh Hajighasemi,Soheila Gharagozlou,Nasrin Moheghi,Roya Ghods,Jalal Khoshnoodi,Fazel Shokri
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Immunology
- نوع مقاله: Journal Article
- کلمات کلیدی: ELISA,Human IgG,Monoclonal antibody,Affinity Constant
- چکیده:
- چکیده انگلیسی: Background: The affinity of an antibody to its antigen is a crucial parameter in its biological activity and performance of an immunoassay such as ELISA. Affinity of most IgG specific MAbs are often determined by methods which require labeling of either antigen or antibody, and are sometimes difficult to control, do not always lead to the expected signal and often result in immunological modification of the molecules. Moreover, direct solid phase binding assays pose some problems such as diffusion effects and difficulties in reaching equilibrium due to heterogeneous binding and co-operativity.
Objective: To employ a rapid and simple ELISA-based method for measuring affinity constants of two pan-h-IgG specific MAbs (3F2D8 and 5F19G11) established in our laboratory.
Methods: The method is based on the effect of antibody affinity on the sigmoidal dose response curve. In this method, the binding of anti-human IgG (anti-h-IgG) MAbs with their corresponding antigen was measured using serial concentrations of both antigen and antibody. The amount of antibody bound to the antigen on the plate is represented as a sigmoidal curve of OD versus the logarithm of antibody concentration added to each well.
Results: Based on the data obtained from this study, the affinity constants of 3F2D8 and 5F19G11 MAbs were 0.74 x 10 8 Mol –1 and 0.96 x 10 7 Mol –1, respectively.
Conclusion: 3F2D8 MAb with reasonably high affinity is suggested as a candidate for quantitative measurement of IgG by ELISA, whereas 5F19G11 MAb could be considered as a suitable tool for immunoaffinity chromatography.- انتشار مقاله: 15-05-1395
- نویسندگان: Fatemeh Hajighasemi,Ali Akbar Saboor-Yaraghi,Fazel Shokri
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Immunology
- نوع مقاله: Journal Article
- کلمات کلیدی: Hepatitis B,neonate,Ant i-HBs antibody,Vaccinat ion,Immunogenicit y,Seroprotection
- چکیده:
- چکیده انگلیسی:
Objective: To compare immunogenicity of a recombinant hepatitis B (HB) vaccine in two groups of neonates born in two cities of Iran with different geographic and ethnic backgrounds. Materials and
Methods: Ten micrograms of a recombinant HB vaccine was administered under field condition to Iranian healthy neonates at 0, 1.5 and 9 months intervals. The subjects consisted of two groups of 290 and 231 neonates selected from two cities located at north-west (Urmia) and south-east (Kerman) of Iran, respectively. The level of anti-HBs antibody was quantitated in serum 2-4 weeks after administration of the last vaccine dose, by sandwich ELISA.
Results: A higher seroprotection rate (anti-HBs> 10 IU/L) (98.3% vs. 96.1%) and significantly increased serum anti- HBs antibody titer (11869 vs. 6104 IU/L) (P<0.001) were induced in vaccinated neonates from Urmia city, compared to those born in Kerman.
Conclusion: These findings suggest contribution of ethnic and/or environmental factors in the antibody response to recombinant HB vaccine in human.- انتشار مقاله: 15-05-1395
- نویسندگان: Abdollah Jafarzadeh,Jalal Khoshnoodi,Shayesteh Ghorbani,Saleh Mohaghegh Hazrati,Babak Faraj Mazaheri,Fazel Shokri
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Immunology
- نوع مقاله: Journal Article
- کلمات کلیدی: ELISA,Human IgG,Monoclonal antibody,Affinity Constant
- چکیده:
- چکیده انگلیسی: Background: The affinity of an antibody to its antigen is a crucial parameter in its biological activity and performance of an immunoassay such as ELISA. Affinity of most IgG specific MAbs are often determined by methods which require labeling of either antigen or antibody, and are sometimes difficult to control, do not always lead to the expected signal and often result in immunological modification of the molecules. Moreover, direct solid phase binding assays pose some problems such as diffusion effects and difficulties in reaching equilibrium due to heterogeneous binding and co-operativity.
Objective: To employ a rapid and simple ELISA-based method for measuring affinity constants of two pan-h-IgG specific MAbs (3F2D8 and 5F19G11) established in our laboratory.
Methods: The method is based on the effect of antibody affinity on the sigmoidal dose response curve. In this method, the binding of anti-human IgG (anti-h-IgG) MAbs with their corresponding antigen was measured using serial concentrations of both antigen and antibody. The amount of antibody bound to the antigen on the plate is represented as a sigmoidal curve of OD versus the logarithm of antibody concentration added to each well.
Results: Based on the data obtained from this study, the affinity constants of 3F2D8 and 5F19G11 MAbs were 0.74 x 10 8 Mol –1 and 0.96 x 10 7 Mol –1, respectively.
Conclusion: 3F2D8 MAb with reasonably high affinity is suggested as a candidate for quantitative measurement of IgG by ELISA, whereas 5F19G11 MAb could be considered as a suitable tool for immunoaffinity chromatography.- انتشار مقاله: 15-05-1395
- نویسندگان: Fatemeh Hajighasemi,Ali Akbar Saboor-Yaraghi,Fazel Shokri
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Immunology
- نوع مقاله: Journal Article
- کلمات کلیدی: Hepatitis B,neonate,Ant i-HBs antibody,Vaccinat ion,Immunogenicit y,Seroprotection
- چکیده:
- چکیده انگلیسی:
Objective: To compare immunogenicity of a recombinant hepatitis B (HB) vaccine in two groups of neonates born in two cities of Iran with different geographic and ethnic backgrounds. Materials and
Methods: Ten micrograms of a recombinant HB vaccine was administered under field condition to Iranian healthy neonates at 0, 1.5 and 9 months intervals. The subjects consisted of two groups of 290 and 231 neonates selected from two cities located at north-west (Urmia) and south-east (Kerman) of Iran, respectively. The level of anti-HBs antibody was quantitated in serum 2-4 weeks after administration of the last vaccine dose, by sandwich ELISA.
Results: A higher seroprotection rate (anti-HBs> 10 IU/L) (98.3% vs. 96.1%) and significantly increased serum anti- HBs antibody titer (11869 vs. 6104 IU/L) (P<0.001) were induced in vaccinated neonates from Urmia city, compared to those born in Kerman.
Conclusion: These findings suggest contribution of ethnic and/or environmental factors in the antibody response to recombinant HB vaccine in human.- انتشار مقاله: 15-05-1395
- نویسندگان: Abdollah Jafarzadeh,Jalal Khoshnoodi,Shayesteh Ghorbani,Saleh Mohaghegh Hazrati,Babak Faraj Mazaheri,Fazel Shokri
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Immunology
- نوع مقاله: Journal Article
- کلمات کلیدی: ELISA,Human IgG,Monoclonal antibody,Affinity Constant
- چکیده:
- چکیده انگلیسی: Background: The affinity of an antibody to its antigen is a crucial parameter in its biological activity and performance of an immunoassay such as ELISA. Affinity of most IgG specific MAbs are often determined by methods which require labeling of either antigen or antibody, and are sometimes difficult to control, do not always lead to the expected signal and often result in immunological modification of the molecules. Moreover, direct solid phase binding assays pose some problems such as diffusion effects and difficulties in reaching equilibrium due to heterogeneous binding and co-operativity.
Objective: To employ a rapid and simple ELISA-based method for measuring affinity constants of two pan-h-IgG specific MAbs (3F2D8 and 5F19G11) established in our laboratory.
Methods: The method is based on the effect of antibody affinity on the sigmoidal dose response curve. In this method, the binding of anti-human IgG (anti-h-IgG) MAbs with their corresponding antigen was measured using serial concentrations of both antigen and antibody. The amount of antibody bound to the antigen on the plate is represented as a sigmoidal curve of OD versus the logarithm of antibody concentration added to each well.
Results: Based on the data obtained from this study, the affinity constants of 3F2D8 and 5F19G11 MAbs were 0.74 x 10 8 Mol –1 and 0.96 x 10 7 Mol –1, respectively.
Conclusion: 3F2D8 MAb with reasonably high affinity is suggested as a candidate for quantitative measurement of IgG by ELISA, whereas 5F19G11 MAb could be considered as a suitable tool for immunoaffinity chromatography.- انتشار مقاله: 15-05-1395
- نویسندگان: Fatemeh Hajighasemi,Ali Akbar Saboor-Yaraghi,Fazel Shokri
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Immunology
- نوع مقاله: Journal Article
- کلمات کلیدی: Hepatitis B,neonate,Ant i-HBs antibody,Vaccinat ion,Immunogenicit y,Seroprotection
- چکیده:
- چکیده انگلیسی:
Objective: To compare immunogenicity of a recombinant hepatitis B (HB) vaccine in two groups of neonates born in two cities of Iran with different geographic and ethnic backgrounds. Materials and
Methods: Ten micrograms of a recombinant HB vaccine was administered under field condition to Iranian healthy neonates at 0, 1.5 and 9 months intervals. The subjects consisted of two groups of 290 and 231 neonates selected from two cities located at north-west (Urmia) and south-east (Kerman) of Iran, respectively. The level of anti-HBs antibody was quantitated in serum 2-4 weeks after administration of the last vaccine dose, by sandwich ELISA.
Results: A higher seroprotection rate (anti-HBs> 10 IU/L) (98.3% vs. 96.1%) and significantly increased serum anti- HBs antibody titer (11869 vs. 6104 IU/L) (P<0.001) were induced in vaccinated neonates from Urmia city, compared to those born in Kerman.
Conclusion: These findings suggest contribution of ethnic and/or environmental factors in the antibody response to recombinant HB vaccine in human.- انتشار مقاله: 15-05-1395
- نویسندگان: Abdollah Jafarzadeh,Jalal Khoshnoodi,Shayesteh Ghorbani,Saleh Mohaghegh Hazrati,Babak Faraj Mazaheri,Fazel Shokri
- مشاهده