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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: Research Journal of Pharmacognosy
- نوع مقاله: Journal Article
- کلمات کلیدی: oxidative stress,diabetes,Carvacrol,Alloxan,Nephropathy
- چکیده:
- چکیده انگلیسی: Background and Objectives: Diabetes, a common metabolic disorder, is prevalent in many countries. Nephropathy is a main debate’s side effect. Role of oxidative stress is well known in induction of diabetic nephropathy while carvacrol is a potent anti-oxidant that might attenuate oxidative stress. The aim of this study was to explore the effect of carvacrol in decreasing nephropathy-induced oxidative damage in diabetic rats. Methods: Thirty five Wistar rats (200-250 g) were divided to 7 groups. The rats received alloxan (i.p., 200 mg/kg) for induction of diabetes. After one week, fasting blood sugar (FBS) was assessed and the rats with FBS>250 mg/dL were considered as diabetic. Three weeks after alloxan injection, the blood urea (BUN) and creatinine (Cr) were determined for confirmation of inducing nephropathy. Then, the animals were treated with carvacrol for one week. Finally, they were anesthetized and blood was collected from animal’s heart for calculation of BUN and Cr. Furthermore, the kidneys were for oxidative stress markers such as glutathione capacity, protein carbonyl, lipid peroxidation and catalase activity. Results: Our results showed that glutathione level and catalase activity significantly increased after treatment with carvacrol. Same results were found in rats that received vitamin E. Also, lipid peroxidation, protein carbonyl content, BUN and Cr levels significantly decreased after treatment with carvacrol in comparison with diabetic rats. Conclusion: Our results showed that carvacrol improved nephropathy-induced oxidative damage similar to vitamin E. Therefore, it may be suggested that carvacrol can be suggested as a useful supplement in decreasing diabetic complaints along with anti-diabetic drugs.
- انتشار مقاله: 15-07-1396
- نویسندگان: Hamid Reza Jamshidi,Zahra Zeinabady,Ehsan Zamani,Mohammad Shokrzadeh,Fatemeh Shaki*
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Plant Physiology
- نوع مقاله: Journal Article
- کلمات کلیدی: fatty acids,Safflower,Salinity,phytohormones,Penconazole
- چکیده:
- چکیده انگلیسی: Salinity of soil is a major abiotic stress limiting the crop production and growth of safflower. To mitigate stress, the effects of penconazole (PEN) on the growth of safflower were studied to understand the underlying mechanisms of salt tolerance. PEN, a triazole derivative, which has both fungicidal and plant growth regulator properties, protects plants from several types of abiotic stresses. The purpose of this work is to assess the effect of sodium chloride (0, 100, and 200 mM) and PEN (15 mg/L) on some biochemical responses of safflower. Results revealed that salicylic acid (SA) content increased under salinity however, indol-3-acetic acid (IAA) and gibberellic acid (GA) contents decreased. Further, in terms of fatty acids, palmitic and oleic acids contents decreased, while stearic, linoleic and linolenic acids content increased under salinity. Exogenous PEN had a positive effect on SA and GA contents, as well as palmitic and stearic acids content, but decreased IAA, linoleic acid and linolenic acid contents in safflower plants. Safflower is a viable alternative for use in rotations where saline irrigation water limits productivity of non-tolerant crops. Our data provide new insights to mechanisms that help regulate salinity resistance in safflower. PEN may be considered as a foliar application to ameliorate salinity effects, due to its low price and availability.
- انتشار مقاله: 10-04-1398
- نویسندگان: Fatemeh Shaki,Vahid Niknam,Hasan Ebrahimzadeh Maboud
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Pharmaceutical Research
- نوع مقاله: Journal Article
- کلمات کلیدی: Mitochondria,Antioxidant,Nephrotoxicity,Protection,Depleted uranium,Beta-glucan
- چکیده:
- چکیده انگلیسی: Considerable evidence suggests that mitochondrial dysfunction contributes to the toxicity of uranyl acetate (UA), a soluble salt of depleted uranium (DU). We examined the ability of the two antioxidants, beta-glucan and butylated hydroxyl toluene (BHT), to prevent UA-induced mitochondrial dysfunction using rat-isolated kidney mitochondria. Beta-glucan (150 nM) and BHT (20 nM) attenuated UA-induced mitochondrial reactive oxygen species (ROS) formation, lipid peroxidation and glutathione oxidation. Beta-glucan and BHT also prevented the loss of mitochondrial membrane potential (MMP) and mitochondrial swelling following the UA treatment in isolated mitochondria. Our results show that beta-glucan and BHT prevented UA-induced mitochondrial outer membrane damage as well as release of cytochrome c from mitochondria. UA also decreased the ATP production in isolated mitochondria significantly inhibited with beta-glucan and BHT pre-treatment. Our results showed that beta-glucan may be mitochondria-targeted antioxidant and suggested this compound as a possible drug candidate for prophylaxis and treatment against DU-induced nephrotoxicity.
- انتشار مقاله: 19-06-1391
- نویسندگان: Fatemeh Shaki,Jalal Pourahmad
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Basic Medical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: oxidative stress,Urtica dioica,diabetes,Nephropathy,Pioglitazone,Stereptozocin
- چکیده:
- چکیده انگلیسی: Objective(s): Hyperglycemia promotes oxidative stress that plays a crucial role in the pathogenesis of Diabetic nephropathy (DN). In this study, we investigated the synergism effects of hydroalcoholic extract of Urtica dioica and pioglitazone (PIO) on the prevention of DN in streptozotocin induced-diabetic mice.
Materials and Methods: Forty-two mice were divided into six groups as follows: non-diabetic control group, DMSO group (as solvent), diabetic group and four treatment groups which received U. dioica, pioglitazone, U. dioica plus pioglitazone and vitE. Diabetes was induced by a single dose of streptozotocin (STZ) (200 mg/kg body wt, IP) diluted in citrate buffer (pH= 4.6). After 4 weeks treatment, all animals were anaesthetized and blood was collected for serum urea and creatinine levels assessment in plasma and kidney tissue were excised for evaluation of oxidative stress markers.
Results: Treatment with U. dioica significantly inhibited increase in serum urea and creatinine in plasma that were observed in diabetic mice. Furthermore, the elevated level of oxidative stress markers (glutathione oxidation, lipid peroxidation (LPO), protein carbonyl) in renal supernatant of diabetic mice was inhibited by U. dioica treatment. Interestingly, U. dioica promoted beneficial effects of PIO in reducing STZ-induced hyperglycemia, renal damage and oxidative stress markers.
Conclusion: Our findings showed that PIO plus U. dioica have synergism protective effects against STZ-induced nephropathy that can be a candidate as a therapeutic approach in order to treatment of DN.- انتشار مقاله: 12-02-1396
- نویسندگان: Mohammad Shokrzadeh,Sara Sadat-hosseini,Marjan Fallah,Fatemeh Shaki
- مشاهده
- جایگاه : پژوهشی
- مجله: International Journal of Pediatrics
- نوع مقاله: Journal Article
- کلمات کلیدی: Dental caries,Nitric oxide,Total Antioxidant Capacity,pH,Total protein
- چکیده:
- چکیده انگلیسی: Background: Dental caries is one of the most common oral diseases in pre-school children. Several factors can affect caries process. Aim of this study was comparison of some of the chemical properties of saliva such as total antioxidant capacity, total protein, pH, nitric oxide level in caries free (CF), and caries active (CA) children.
Materials and Methods
This cross-sectional based study was designed with random selection of 80 healthy population including 40 CF and 40 CA children (3-5 years old) from several public kindergartens in Sari, Iranin 2019. Caries status was assessed using DMFT (Decayed/Missing/Filled Teeth) index according to WHO criteria. Un-stimulated saliva samples were collected from children in the morning. Then, several caries-related factors including total antioxidant capacity, nitric oxide, total protein concentration and pH were assessed in saliva samples. Data were analyzed using SPSS software version 16.0.
Results: Significant higher total antioxidant capacity and total protein concentration were observed in the saliva of CA than in the CF children. On the other hand, nitric oxide level was markedly lower in CA samples. In addition, a decrease in pH of saliva was observed in CA children.
Conclusion
Based on the results, increase in the total antioxidant capacity and total protein as well as decrease in nitric oxide levels in the saliva of CA children can be considered as valuable evidence of dental caries occurrence among children.- انتشار مقاله: 13-06-1398
- نویسندگان: Fatemeh Shaki,Milad Arab-Nozari,Faezeh Maleki,Jamshid Yazdani Charati,Azam Nahvi
- مشاهده
- جایگاه : پژوهشی
- مجله: Addiction and Health
- نوع مقاله: Journal Article
- کلمات کلیدی: Tramadol,Melatonin,oxidative stress,Mitochondria,Apoptosis
- چکیده:
- چکیده انگلیسی: Background: The aim of the present study was to investigate the protective properties of melatonin (MT)
against oxidative stress, mitochondrial dysfunction, and apoptosis induced by tramadol-reproductive toxicity
in male rats.
Methods: The rats were divided into the 7 groups of control, melatonin (1.5 mg/kg), tramadol (50 mg/kg), and
melatonin (1, 1.5 and 2.5 mg/kg) administered 30 minutes before tramadol and vitamin C group (100 mg/kg).
All injections were performed intraperitoneally. After administration for 3 consecutive weeks, the animals were
killed and testis tissues were used for assessment of oxidative stress markers including lipid peroxidation
(LPO), glutathione (GSH) content and protein carbonyl (PrC), and sperm analysis. Mitochondria were isolated
from rat’s testis using differential centrifugation technique and were studied in terms of mitochondrial
viability, mitochondrial membrane potential (MMP), and mitochondrial swelling. The other part of the tissue
sample was placed in RNA protector solution for assessment of Bax and Bcl-2 gene expression through realtime polymerase chain reaction (real-time PCR) assay.
Findings: Tramadol caused a significant decline in epidermal sperm count, motility, and morphology, as well
as a significant decrease in GSH level and mitochondrial function, and a significant evaluation of LPO, PrC,
MMP, and mitochondrial swelling. In addition, tramadol induced a significant decrease in Bcl-2 gene
expression, and increase in Bax gene expression. However, pretreatment of rats with MT improved sperm
analysis, and testicular antioxidative status, and mitochondrial function. Furthermore, MT pretreatment
regulated testicular Bcl-2 and Bax expressions.
Conclusion: Considering the protective effects of MT against reproductive toxicity induced by tramadol, this
compound can be used as a possible agent for the prevention and treatment of tramadol-induced reproductive
toxicity.
- انتشار مقاله: 11-04-1399
- نویسندگان: Motahareh Koohsari,Nematollah Ahangar,Ebrahim Mohammadi,Fatemeh Shaki
- مشاهده