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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: Nanomedicine Journal
- نوع مقاله: Journal Article
- کلمات کلیدی: Antibacterial activity,Biosynthesis,selenium nanoparticles,Nanobiotechnology
- چکیده:
- چکیده انگلیسی: Objective(s): This study deals with mycosynthesis and characterization of selenium nanoparticles (SeNPs) using the Penicillium chrysogenum PTCC 5031 and evaluating their antibacterial activity.
Materials and Methods: The formation of SeNPs was confirmed with the color change from pale yellow to orange. Tyndall effect also confirmed the formation of colloidal systems through the samples. The SeNPs were characterized using different analytical techniques including photon correlation spectroscopy (PCS), Scanning Electron Microscope (SEM), Atomic Force Microscope (AFM), Energy Dispersive X-ray (EDX), X-ray diffraction (XRD) and Fourier Transform Infrared (FT-IR) analysis.
Results: Our findings revealed that SeNPs were fairly uniformed with good monodispersity and the lesser aggregation of particles in pH value of 7 with the average hydrodynamic size of 24.65 nm, polydispersity index (PdI) of 0.392 and zeta potential of -34 mV. The SeNPs revealed antibacterial activity against gram positive bacteria including Staphylococcus aureus, and Listeria monocytogenes with the zone of inhibition (ZOI) of 10 and 13 mm, respectively.
Conclusion: The results of this study provided a potential solution to the growing need for the development of cost-effective and eco-friendly ways of nanoparticle synthesis to overcome the microbial resistance and control the infectious diseases. However, further investigations are required to demonstrate the efficacy of SeNPs through in vivo models.- انتشار مقاله: 02-07-1399
- نویسندگان: Hossein Vahidi,Farzad Kobarfard,Zahra Kosar,Mohammad Ali Mahjoub,Muthupandian Saravanan,Hamed Barabadi
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Pharmaceutical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: Gas chromatography,Determination,Valproic acid,Human plasma
- چکیده:
- چکیده انگلیسی: A simple, reproducible and rapid gas chromatographic method for precise determination of valproic acid (VPA) in human plasma has been developed. Total time for sample preparation and GC analysis is less than 45 min. After plasma protein precipitation, VPA was extracted into chloroform with suitable recovery. By using Stabilwax®-DA capillary GC column, a symmetrical gas chromatographic peak was obtained without the need for derivatization. The calibration curve was proved to be linear (r2 = 0.998) in a wide concentration range (0.45-100 μg/ml). Inter-day and intra-day accuracy and precision of this method was investigated during the method validation and the method has good precision and accuracy. This method is highly reproducible with a limit of detection 150 ng/ml of VPA in human plasma and could be used in TDM and pharmacokinetic studies.
- انتشار مقاله: 20-05-1385
- نویسندگان: Reza Ahmadkhaniha,Noushin Rastkari,Farzad Kobarfard,Hossein Pakdaman,Omid Ahmadkhaniha,Abbas Kebriaeezadeh
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Pharmaceutical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: Cytotoxicity,Tautomerization,Cytochrome P450,Dacarbazine,Lysosomes Oxidative stress
- چکیده:
- چکیده انگلیسی: Dacarbazine (DTIC) is a synthetic chemical antitumor agent which is used to treat malignant melanoma and Hodgkin’s disease. DTIC is a prodrug which is converted to an active form undergoing demethylation by liver enzymes. The active form prevents the progress of disease via alkylation of DNA strand. In the structure of this drug, the imidazole ring, a triazen chain and carboxamide group exist. Based on the literature, the ring and carboxamide group do not have a key role in antitumor activity of the drug. On the other hand, imidazole ring has a unique tautomeriza-tion which may participate in the mechanism of action of DTIC and carboxamide group may determine the rich guanine pieces in DNA strand. In order to investigate the mechanistic role of imidazole group and its known tautomerization in DTIC cytotoxicity, derivative of DTIC with a pyridine ring (3-(3,3-dimethyl-1-triazenyl)pyridine, (compound I) instead of imidazole ring was synthesized. In the following, the cellular and molecular mechanism of cytotoxicity induced by DTIC and its pyridine derivative toward the isolated rat hepatocytes were studied and compared. Hepatocyte reactive oxygen species (ROS) generation was significantly increased by both DTIC and compound B before cytotoxicity ensued. In addition, DTIC and compound I induced lysosomal damage and hepatocyte protease activation. Endocytosis inhibitors, lysosomotropic agents or lysosomal protease inhibitors also prevented both DTIC and compound B induced hepatocytes cytotoxicity. Furthermore desferoxamine (a ferric chelator), antioxidants or ROS scavengers (catalase, mannitol or dimethylsulfoxide) prevented both DTIC and compound I cytotoxicity. It is concluded that H2O2 reacts with lysosomal Fe2+ to form hydroxyl radical which (Haber-Weiss reaction) causes lysosomal membrane disruption, proteases and other digestive enzymes release and finally the cell death.
- انتشار مقاله: 20-02-1385
- نویسندگان: Jalal Pourahma,Farzad Kobarfard,Marzieh Amirmostofian
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Biotechnology
- نوع مقاله: Journal Article
- کلمات کلیدی: Cytotoxicity,Liposomes,Eptifibatide,Materials Testing
- چکیده:
- چکیده انگلیسی: Background: Eptifibatide (Integrilin®) is a hepta-peptide drug which specifically prevents the aggregation of activated platelets. The peptide drugs are encapsulated into nanolipisomes in order to decreasing their side effects and improving their half-life and bioavailability.
Objectives: In this study, the in vitro cytotoxicity and hemocompatibility of RGD-modified nano-liposomes (RGD-MNL) encapsulated a highly potent antiplatelet drug (eptifibatide) was investigated.
Material and Methods: RGD-MNL encapsulated eptifibatide was prepared using lipid film hydration and freeze/thawing method. The morphology and size distribution (about 90 nm) of RGD-MNL were characterized using transmission electron microscopy (TEM). The in-vitro cytotoxicity of nano-liposomes was examined using the MTT, LDH release and reactive oxygen species (ROS) generation assays. The effect of RGD-MNL on red blood cells (RBC) was investigated using hemolysis and LDH release assays.
Results: The results revealed that RGD-MNL had no significant cytotoxic effect on HeLa and HUVEC cell lines, and also no ROS generation increase in the cells. In addition, the adverse effect of RGD-MNL on LDH release and membrane integrity of RBC was not observed.
Conclusions: In conclusion, the recommended RGD-MNL formulations have not any significant cytotoxicity on normal cells or RBC and have potential for protecting and enhancing the activity of antiplatelet drugs.- انتشار مقاله: 29-07-1396
- نویسندگان: Hassan Bardania,Seyed Abbas Shojaosadati,Farzad Kobarfard,Dina Morshedi,Farhang Aliakbari,Mohammad Taher Tahoori,Elahe Roshani
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Biotechnology
- نوع مقاله: Journal Article
- کلمات کلیدی: Eptifibatide,RGD-modified nano-liposomes (RMNL),RMNL encapsulated eptifibatide
- چکیده:
- چکیده انگلیسی: Background: Eptifibatide (Integrilin) is an intravenous (IV) peptide drug that selectively inhibits ligand binding to the platelet GP IIb/IIIa receptor. It is an efficient peptide drug, however has a short half-life. Therefore, antithrombotic agents like eptifibatide are required to become improved with a protected and targeted delivery system such as using nano-liposomes to the site of thrombus. Objectives: The goal in the present report was to optimize encapsulation efficiency of the eptifibatide into Arg-Gly-Asp (RGD)-modified nano-liposomes (RMNL). As well, it was intended to evaluate the effect of sodium lauryl sulfate (SLS) on drug release. Materials and Methods: The effect of five independent variables including number of freeze/thawing cycles, concentration of eptifibatide, 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC), cholesterol, and dipalmitoyl-GRGDSPA peptide on drug entrapment efficiency (DEE) was investigated using response surface methodology (RSM). The effect of different concentrations of SLS on encapsulation and drug release from RMNL was also investigated. The size and morphology of RMNL were characterized using transmission electron microscopy (TEM). Results: The maximum DEE (38%) was obtained with 7 freeze/thawing cycles, 3.65 mmoL eptifibatide, 7 mM DSPC, 3 mM cholesterol, and 1 mM dipalmitoyl- GRGDSPA peptide. SLS has significantly increased the drug release from RMNL, although its effect on encapsulation efficiency was not significant. Conclusions: The optimization of the formulations for valuable and expensive peptide drugs is essential to have the maximum encapsulation efficiency and the minimum experiments.
- انتشار مقاله: 26-08-1394
- نویسندگان: Hassan Bardania,Seyed Abbas Shojaosadati,Farzad Kobarfard,Farid Dorkoosh
- مشاهده