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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: Iranian Journal of Pathology
- نوع مقاله: Journal Article
- کلمات کلیدی: immunohistochemistry,Hirschsprungs’s disease,Allied disorders,Neuron-specific enolase
- چکیده:
- چکیده انگلیسی: Background and Objective: Diagnosis of Hirschsprung’s disease (HD) as the most common cause of neonatal intestinal obstruction is based on the presence of aganglionosis from seromuscular or full thickness biopsy. Due to the complication of full thickness or seromuscular rectal biopsy, mucosal-sub mucosal biopsy is more intended. However, interpretation of these biopsies stained with hematoxylin and eosin (H&E) and even using immunohistochemical (IHC) methods such as acetylcholine esterase is often problematic. Although neuron-specific enolase staining (NSE) is an available and easy method to perform for diagnosis of HD, however, our knowledge on its specificity is not adequate. Therefore, this study was aimed to determine the diagnostic value of NSE on the mucosal-sub mucosal rectal biopsy for the diagnosis of HD and the allied disorders deficit. Materials and Methods: This study was conducted on 65 mucosal-submucosal and 65 seromuscular rectal biopsies (standard) obtained from the patients suspected of HD and allied disorders referred to the Avicena and Shafa hospitals (Sari, Iran) from April 2003 to September 2004. Two biopsies were taken from each patient: the mucosal-submucosal biopsy was stained by NSE and H&E staining was used for seromuscular samples. The prepared slides were observed and evaluated at double blind condition and the results were compared. Results: Sensitivity, specificity, efficiency, positive and negative predictive values in the diagnosis of HD in NSE method were 100%, 84.2%, 89.1%, 81.8%, and 100% respectively (p<0.05). On evaluation of hypoganglionosis, there were one false-negative and nine false-positive. Conclusion: In NSE staining, finding ganglion cell definitely rules out HD, but lack of ganglion cell confirms 81.8% of H.D cases. Thus, NSE staining on mucosal- submucosal specimens is possibly adequate for establishing the presence or absence of ganglion cells.
- انتشار مقاله: 06-07-1393
- نویسندگان: Zhila Torabizadeh,Farshad Naghshvar,Ali Reza Khalilian
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Basic Medical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: Nephrotoxicity,MDMA,Feijoa sellowiana,Kidney glutathione
- چکیده:
- چکیده انگلیسی:
Objective(s): Because of the high total phenolic contents of Feijoa sellowiana
, its nephroprotective effect was determined in 3,4-methylene dioxymethamphetamine (MDMA) treated mice.
Materials and Methods:
Animals received 10, 20 and 40 mg/kg doses of aqueous or methanol extract of F. sellowiana
leaves, intra-peritoneally. After one hour, an acute administration of MDMA (20 mg/kg, IP) was injected. Nephroprotective effect was assayed by determination of serum creatinine, serum urea and kidney glutathione level. Histopathological study was also used.
Results
: Both extracts at 40 mg/kg resulted in a significant reversal in the raised serum creatinine levels (P <0.05). Not statistically significant was observed in effect of two extracts (P>0.05). A decrease in urea/ creatinine ratio was observed following aqueous extract treatment. Methanolic extract showed higher activity in increasing kidney glutathione (P
Conclusion:
F. sellowiana extract resulted in a markedly decrease in the nephrotoxicity of MDMA in mice.- انتشار مقاله: 05-11-1392
- نویسندگان: Mohammad Karami,Faezeh Karimian Nokabadi,Mohammad Ali Ebrahimzadeh,Farshad Naghshvar
- مشاهده
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: Breast cancer,Sentinel Lymph Node Biopsy,frozen section,false negative rate
- چکیده:
- چکیده انگلیسی: Background: Improvements in the process of staging and surgical treatment of axillary lymph nodes in recent years, have led to the use of intra operative frozen section pathology to examine the sentinel lymph node biopsy in breast cancer patients. Materials and Methods: we evaluated the results of the Sentinel biopsy in 102 patients with early stage breast cancer, which were negative clinical lymph nodes, and analyzing the true positive and false negative rate, diagnostic accuracy of frozen section lymph node biopsy. It also studied the factors affecting the sentinel and non-sentinel lymph nodes in patients treated by axillary lymph dissection. Results: In this study, we investigated 102 patients’ stage 1and 2 breast cancer with clinical negative axillary lymph node and candidates for sentinel lymph node biopsy, were placed under investigation. 15.7 % of the real positive results of sentinel and 62.7 % of the real negative and 2 % false positives and 20.9 % false negative results and% 78. 4 diagnostic accuracy, has been frozen section. Among the patients who were initially or delayed in the axillary dissection, 37% had more than two lymph nodes. While in general, 16.7% of patients had a need for axillary lymph node dissection based on z11 criteria. Lymph-vascular invasion was a major contributor to lentil involvement in Sentinel and non-Sentinel nodes. Conclusion: Frozen section pathology during the operation of sentinel lymph node biopsy has been initiated to prevent the need for a reoperation in early stage breast cancer patients. However, due to low tumor burden in patients who are candidates for this procedure, and the constraints in the initial sections and their false negative results, also the removal of frozen section will not have an effect on the rate of increasing reoperation and can be effective in reducing the time and cost of surgery.
- انتشار مقاله: 27-06-1398
- نویسندگان: Gholamali Godazande,Siavash Moradi,Farshad Naghshvar,Leyla Shojaee
- مشاهده