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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: Progress in Biological Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: Iran,Karyology,Muridae,Rodentia
- چکیده:
- چکیده انگلیسی: Family Muridae consists of eight genera and 28 species in Iran. This family shows notable variability of karyological features. In this study, karyological data derived from 11 species of 7 genera of this family of Iran are presented and compared with previously published accounts. The included species were: Mus musculus musculus from Gonbad (2n=40, FN=40) and Birjand (2n=40, FN=38), Rattus norvegicus (2n=42), Apodemus uralensis (2n=48), Apodemus avicennicus (2n=48), Apodemus hyrcanicus (2n=48), Apodemus witherbyi (2n=48) from Zanjan and Gorgan, Nesokia indica (2n=42), Meriones libycus (2n=44), Meriones persicus (2n=44), Meriones crassus from Kashmar (2n=54) and Tabas (2n=60), Tatera indica from Zabul (2n=68) and Hoveyzeh (2n=66) and Rhombomys opimus (2n=40).
- انتشار مقاله: 11-10-1348
- نویسندگان: Zeinolabedin Mohammadi,Jamshid Darvish,Farhangh Haddad,Fatemeh Ghorbani
- مشاهده
- جایگاه : پژوهشی
- مجله: Progress in Biological Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: Iran,Karyology,Muridae,Rodentia
- چکیده:
- چکیده انگلیسی: Family Muridae consists of eight genera and 28 species in Iran. This family shows notable variability of karyological features. In this study, karyological data derived from 11 species of 7 genera of this family of Iran are presented and compared with previously published accounts. The included species were: Mus musculus musculus from Gonbad (2n=40, FN=40) and Birjand (2n=40, FN=38), Rattus norvegicus (2n=42), Apodemus uralensis (2n=48), Apodemus avicennicus (2n=48), Apodemus hyrcanicus (2n=48), Apodemus witherbyi (2n=48) from Zanjan and Gorgan, Nesokia indica (2n=42), Meriones libycus (2n=44), Meriones persicus (2n=44), Meriones crassus from Kashmar (2n=54) and Tabas (2n=60), Tatera indica from Zabul (2n=68) and Hoveyzeh (2n=66) and Rhombomys opimus (2n=40).
- انتشار مقاله: 11-10-1348
- نویسندگان: Zeinolabedin Mohammadi,Jamshid Darvish,Farhangh Haddad,Fatemeh Ghorbani
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Basic Medical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: Gamma Irradiation,micronucleus,Lymphocytes,green tea,Natural radioprotective
- چکیده:
- چکیده انگلیسی: Objective(s)
The aim of this study was to investigate the radioprotective effect of green tea against genotoxicity induced by gamma irradiation in cultured blood lymphocytes from 5 human volunteers.
Materials and Methods
Peripheral blood samples were collected from volunteers before and 1, 3 and 5 hr after drinking a decoction 4 g green tea in 280 ml boiling water for 5 constitutive days with the same quantity. At each time point, the whole blood samples were exposed to 200 cGy of 60 Co gamma irradiation and then were cultured with mitogenic stimulation to determine the chromosomal aberration in micronucleus assay on cytokinesis-blocked binucleated cells.
Results
As expected, for each volunteer, the results showed a significant increase in the incidence of micronuclei after exposure to gamma irradiation as compared to non-irradiated control samples. Only lymphocytes blood sample collected 3 hr after drinking green tea exhibited a significant decrease in incidence of micronuclei compared to non-treated irradiated samples.
Conclusion
These results suggest the radioprotective ability of green tea against ionizing radiation in human lymphocytes, at specified time after consumptior.- انتشار مقاله: 26-06-1394
- نویسندگان: Hafezeh Davari,Farhang Haddad,Ali Moghimi,Mohammad Farhad Rahimi,Mohammad Reza Ghavamnasiri
- مشاهده
- جایگاه : پژوهشی
- مجله: Avicenna Journal of Phytomedicine
- نوع مقاله: Journal Article
- کلمات کلیدی: micronucleus,Origanum vulgare,Radioprotective Agents,Bone marrow cells,Whole-body Irradiation
- چکیده:
- چکیده انگلیسی:
Objective: Ionizing radiation produces free radicals which induce DNA damage and cell death. Origanum vulgare leaf extract (OVLE) is a natural compound and its capability of scavenging free radicals and its antioxidant activity have been demonstrated by many researchers. In this study, using micronucleus assay, radioprotective effect of OVLE against clastogenic and cytotoxic effect of gamma irradiation has been investigated in mice bone marrow cells.
Materials and Methods: OVLE was injected intraperitoneally to the BALB/c mice 1hr prior to gamma irradiation (3Gy) at the doses of 100 and 200 mg/kg. Twenty four hours after irradiation or treatment, animals were killed and smears were prepared from the bone marrow cells. The slides were stained with May Grunwald–Giemsa method and analyzed microscopically. The frequency of micronucleated polychromatic erythrocytes (MnPCEs), micronucleated normochromatic erythrocyte (MnNCEs) and cell proliferation ratio PCE/PCE+NCE (polychromatic erythrocyte/polychromatic erythrocyte + normochromatic erythrocyte) were calculated.
Results: The results showed that gamma irradiation (3Gy) increased the frequency of MnPCEs, MnNCEs and reduced the PCE/PCE+NCE ratio in mice bone marrow compared to the non-irradiated control group (p< 0.0001). Injection of OVLE significantly reduced the frequency of MnPCEs (p< 0.0001) and MnNCEs (p< 0.05) and increased the PCE/PCE+NCE ratio as compared to the irradiated control group (p< 0.05).
Conclusion: It seems that OVLE with its antioxidant properties and its capability of scavenging free radicals and reactive oxygen species can reduce the cytotoxic effects of gamma irradiation in mice bone marrow cells.- انتشار مقاله: 11-09-1394
- نویسندگان: Reza Ghasemnezhad Targhi,Vahid Changizi,Farhang Haddad,Mansour Homayoun,Shokohozaman Soleymanifard
- مشاهده
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: Gamma Irradiation,Vinblastine,micronucleus assay,L929 cell
- چکیده:
- چکیده انگلیسی: Objectives: Vast number of studies show the relationship between aneuploidy and cancer. Ionizing radiation in
addition to induce all kinds of damages to the cells and structure of chromosomes, is also able to induce aneuploidy
through direct damages to chromosome division apparatus. Also irradiation of the cells induces mutations in several
genes which might be involved in cell division fidelity and play a role in reversing the effect of aneugens. Therefore,
irradiation of cells and tissues might produce sensitivity to agents with aneugenic capability in irradiated cells. Methods:
To investigate the persistent genomic effect of ionizing irradiation on chromosomal instability, L929 cells were gamma
irradiated with the dose of 2 Gy. Cells were left to recover from the harmful effect of irradiation. They were treated with
low dose of vinblastine (0.5 ng.ml-1) 72h post-gamma irradiation. Finally, the induced chromosomal abnormalities
were scored using micronucleus assay in cytokinesis-blocked binucleated cells (MnBi). Results: Irradiation-recovered
L929 cells treated with vinblastine showed a statistically higher frequency of MnBi compared to non-irradiated and
vinblastine treated cells. Conclusion: The results indicate that gamma irradiation, in addition to direct induction of
chromosomal damages, is also able to create persisting genomic sensitivity in the cells to chromosomal instability,
which is detectable when exposed to the second stimulus.- انتشار مقاله: 28-11-1396
- نویسندگان: Zahra Mohammadi,Farhang Haddad,Maryam M Matin,Shokouhozaman Soleymanifard
- مشاهده